FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177084
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC1967011.107722888572654No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC165849.365047096293285No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT138877.842041065257166No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA96455.446567730568544No Hit
GGTAGGATGTGATATTCCTTCTAGTGGTACGGTAGCAGAAAGTCAGTCCC89965.080074992658852No Hit
ACCTTGTCACCAATACCTCACATACGGTAGCAGCATCTTGAATCTCATAC54563.0810236949696193No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA51172.8895891215468366No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG45792.585778500598586No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTATGC42362.392085112150166No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT36122.0397099681507083No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC30751.7364640509588671No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTATGCTGGC28981.6365114860744052No Hit
TCTCCCCCACCCCCTCTACACTGACGACATGGTCATTCTCTCCTGGCTCC28361.6014998531770235No Hit
TTAAATTTCACTCCACTTACCTACCAGTCAAATGTCCATGGATCATAGAC27491.5523706263694066No Hit
TGCAGATTTCATGTGCCTAGAAGTTACGGTAGCAAAGGCCAATCAAGTGA26861.5167942897156153No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC26121.4750062117413205No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA25091.4168417248311536No Hit
TGGTGATAGATGACTTGCTGCTACACTGACCCATGTGGAATAGTTCCCAG23911.350206681574846No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCGAGACCAAGT21651.2225836326263242No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC17921.0119491314856226No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTATG16550.9345847168575364No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTATGCT16520.9328906055883084No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC16230.916514196652436No Hit
GGATCCTGGGTGTTTGTATTTGCTACCGTATGGGAGGAGGGCAGTACACA12820.7239502157168349No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTATGCTG12730.7188678819091504No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTATGC12440.7024914729732782No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTATGCT12180.6878091753066341No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTATGCTGG11910.6725621738835807No Hit
TACTGAATGCAAAGGACACCACATTTAGCTGGGACACATAGACCAAGTCT11190.6319035034221048No Hit
GATTCCCGCGCCCAGAGTCCCACGACCCCCTCAGACCAAGTCTCTGCTAC10200.5759978315375754No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAT10040.5669625714350252No Hit
GCAGCTTCTGCCATCTCTCTGCTACCGTAAGAACGTGGGAGTAGACGGAA9690.5471979399606967No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG9490.5359038648325088No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTATGCTG8980.5071039732556301No Hit
GGGAAAAGAACAGGCTTCACCTATCATCGTTTTCCAAGTTATTTTTGAGA8230.464751191524926No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACCGAGAC8230.464751191524926No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT8080.45628063517878525No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTATGCTGG7900.44611596756341626No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG7670.43312778116600026No Hit
CCGGTACAAACCTTTCATTGTATTGTCGTTTTAGTTCAACCAGACCAAGT7470.4218337060378126No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTA7360.4156219647173093No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAAAGACCAAGTC6740.38061033181992726No Hit
TCTATTGTGATGGCCACGTTAGACCAAGTCTCTGCTACCGTATGCTGGCT6680.37722210928147093No Hit
GGGAAGCTCTTCATCCTCACGAGACCAAGTCTCTGCTACCGTATGCTGGC6470.3653633303968738No Hit
AACTTGGGCATGGAGCATGTATCAGTGTGTGTGCTGAGGTATGTCAAAGA6380.36028099658918933No Hit
GTCACTGGTTAAAACTAAGGTGGGAGCATTGGTTTAGACCAAGTCTCTGC6310.3563280702943236No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTATGCTGGCTTGATC5940.33543403130717625No Hit
GTGCTGGTAAATTCACTCCATCGAATCATCACCAATAGACCAAGTCTCTG5430.3066341397302975No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA5420.30606943597388814No Hit
TCAATGCAGAGGTTGAAGATGGTACACTGACGACATGGTTCTACATTGTT5080.2868695082559689No Hit
GCAGAGCCAAACCGACCTGAGCATCCCTCAAATGGCACAGCTGCTTAACA4950.27952835942264687No Hit
AATCTCCAAGGAAGTTGTACCGTCTTAGACCAAGTCTCTGCTACCGTATG4790.2704930993200967No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA4670.263716654243184No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT4250.23999909647398976No Hit
TGTCATAATGAATGCCCCATCGTACGGTAGCAGAACTGTCAAATGACCAA4200.23717557769194283No Hit
TGAATTCTCCTCAGATGACTCCATTTAAAGACCAAGTCTCTGCTACCGTA4040.22814031758939263No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT3880.21910505748684242No Hit
CCAGTGTTGTGTTTCCCAAAGTCACTGACGACATGGTTCTACACCAGTGC3330.18804635088432609No Hit
GAAGAGACCAAGCAAGTTTTGAGACACTGACGACATGGTCATTCTCTCCT3330.18804635088432609No Hit
AACAATCGACAGGCTAGAAGTTGAGACCAAGTCTCTGCTACCGTATGCTG3290.18578753585868854No Hit
AAGGGAAAAGGATGGATTAGGGTACACTGACGACATGGTTCTACTACCCT2770.15642294052540037No Hit
AACTGATCACCTTGAATCTGCTTGGAGAAGGAATATTTGATGGTCAACCA2730.15416412549976283No Hit
TTAACTACAATTCCCAGCAAGCGGTCTCTGAGTCGAGACCAAGTCTCTGC2630.14851708793566895No Hit
GACTACAGATGTGCCACCATACCCAGAGACCAAGTCTCTGCTACCGTATG2210.12479953016647466No Hit
ATTTAACACCTAGCCAAAAGGCAGACCAAGTCTCTGCTACCGTATGCTGG2140.12084660387160896No Hit
CACAAAGGTTTTTATAAGTTCTGTGGATCTAGACCAAGTCTCTGCTACCG2040.11519956630751509No Hit
TGATAAATGCACCTGTTTGCTGTCACATTCAGAGACCAAGTCTCTGCTAC2030.11463486255110569No Hit
CTCATTCTTTCCTTGATTTTCTTCCTACGGTAGCAAATACTGAGCATCAA1900.10729371371778365No Hit
CTTTCTAGTTGATACGGCCTTCACAAAGACCAAGTCTCACTGAAAGCCAG1900.10729371371778365No Hit
GTACAATTTCTACCGCTGGTTCCCAGACCAAGTCTCTGCTACCGTATGCT1860.1050348986921461No Hit
ACACCACTCTCTAAATCAAACACATTGGAGACCAAGTCTCTGCTACCGTA1820.10277608366650855No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)