FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177084
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG1962311.081181812021415No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA163789.24871812247295No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC137287.752253167988074No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT94625.343226943145626No Hit
CCATGGGACTGACTTTCTGCTACCGTACCACTAGAAGGAATATCACATCC88324.987463576607712No Hit
GTATGAGATTCAAGATGCTGCTACCGTATGTGAGGTATTGGTGACAAGGT53603.026812134354318No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT50352.843283413521267No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG42372.3926498159065757No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA42342.390955704637347No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT35852.0244629667276546No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG29181.6478055612025932No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC28881.6308644485103114No Hit
TAGGAGCCAGGAGAGAATGACCATGTCGTCAGTGTAGAGGGGGTGGGGGA27491.5523706263694066No Hit
ATGATCCATGGACATTTGACTGGTAGGTAAGTGGAGTGAAATTTAATGTA27071.5286530686002122No Hit
TTCTTCACTTGATTGGCCTTTGCTACCGTAACTTCTAGGCACATGAAATC26051.4710532854464549No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG25771.455241580266992No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT25011.4123240947798785No Hit
GGGAACTATTCCACATGGGTCAGTGTAGCAGCAAGTCATCTATCACCATG23851.3468184590363894No Hit
CGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC21601.2197601138442773No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG17951.0136432427548507No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT16420.9272435680242145No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG16300.9204671229473018No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG16130.9108671590883423No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC12650.7143502518578754No Hit
GTGTACTGCCCTCCTCCCATACGGTAGCAAATACAAACACCCAGGATCCT12580.7103973255630096No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA12580.7103973255630096No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT12160.6866797677938153No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT11890.6714327663707619No Hit
ATGTGTCCCAGCTAAATGTGGTGTCCTTTGCATTCAGTATGTAGAACCAT11050.6239976508323734No Hit
GAGGGGGTCGTGGGACTCTGGGCGCGGGAATCTGTAGAACCATGTCGTCA10160.5737390165119378No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA10050.5675272751914345No Hit
TCCGTCTACTCCCACGTTCTTACGGTAGCAGAGAGATGGCAGAAGCTGCT9610.5426803099094215No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT9320.5263039009735493No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC8970.5065392694992207No Hit
CAAAAATAACTTGGAAAACGATGATAGGTGAAGCCTGTTCTTTTCCCTGT8180.4619276727428791No Hit
CGGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA8120.45853945020442277No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT8030.45345711639673825No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT8000.4517630051275101No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT7590.42861015111472517No Hit
GGTTGAACTAAAACGACAATACAATGAAAGGTTTGTACCGGTGTAGAACC7570.4274807436019064No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT7260.40997492715321543No Hit
AACGTGGCCATCACAATAGATGTAGAACCATGTCGTCAGTGTAGATCTCG6650.3755279980122428No Hit
TTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA6620.37383388674301465No Hit
CGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC6390.3608457003455987No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT6310.3563280702943236No Hit
TTGACATACCTCAGCACACACACTGATACATGCTCCATGCCCAAGTTTGT6290.3551986627815048No Hit
AAACCAATGCTCCCACCTTAGTTTTAACCAGTGACTGTAGAACCATGTCG5930.33486932755076687No Hit
TTCATGGTGTTTTATCCCTCTTGAACAATGTAGAACCATGTCGTCAGTGT5600.3162341035892571No Hit
ATTGGTGATGATTCGATGGAGTGAATTTACCAGCACTGTAGAACCATGTC5350.30211650967902237No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT5330.3009871021662036No Hit
AAGACGGTACAACTTCCTTGGAGATTTGTAGAACCATGTCGTCAGTGTAG4720.266540173025231No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA4710.26597546926882154No Hit
GGTCTCTGAGTCCTGCTGCTGGGAAGTAGCTTCCGTCAGGGCACTGATGG4460.2518578753585869No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT4240.23943439271758038No Hit
TGGTCATTTGACAGTTCTGCTACCGTACGATGGGGCATTCATTATGACAT4230.238869688961171No Hit
TTAAATGGAGTCATCTGAGGAGAATTCATGTAGAACCATGTCGTCAGTGT4010.22644620632016446No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG3880.21910505748684242No Hit
CAACTTCTAGCCTGTCGATTGTTTGTAGAACCATGTCGTCAGTGTAGATC3310.18691694337150733No Hit
TAGGAGCCAGGAGAGAATGACCATGTCGTCAGTGTCTCAAAACTTGCTTG3300.18635223961509792No Hit
TCCCCTGGAAACTGCACTGGTGTAGAACCATGTCGTCAGTGACTTTGGGA3190.18014049829459466No Hit
CCAAAGCAGAAAGGGTAGTAGAACCATGTCGTCAGTGTACCCTAATCCAT2940.16602290438435996No Hit
GGTTGACCATCAAATATTCCTTCTCCAAGCAGATTCAAGGTGATCAGTTT2690.15190531047412528No Hit
CGACTCAGAGACCGCTTGCTGGGAATTGTAGTTAATGTAGAACCATGTCG2590.1462582729100314No Hit
CCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG2520.1423053466151657No Hit
CTGGGTATGGTGGCACATCTGTAGTCTGTAGAACCATGTCGTCAGTGTAG2170.12254071514083713No Hit
GCCTTTTGGCTAGGTGTTAAATTGTAGAACCATGTCGTCAGTGTAGATCT2130.12028190011519957No Hit
AGATCCACAGAACTTATAAAAACCTTTGTGTGTAGAACCATGTCGTCAGT2050.11576427006392447No Hit
CTGAATGTGACAGCAAACAGGTGCATTTATCATGTAGAACCATGTCGTCA1970.11124664001264936No Hit
GGGAACCAGCGGTAGAAATTGTACTGTAGAACCATGTCGTCAGTGTAGAT1940.10955252874342121No Hit
ACCTATGTTTTATCCTGGCTTTCAGTGAGACTTGGTCTTTGTGAAGGCCG1850.10447019493573671No Hit
CCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT1810.10221137991009915No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)