FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100573

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100573
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478650
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC391518.179463073226783No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC337477.0504544030084615No Hit
CACTTAATGAGACCAACAGTAACACCACTGACTCTGATCCAGAAGACCAA291916.098610675859187No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT263825.511751801942964No Hit
TGAACTTATATATTTGACACAACCCAGCCATGACGACATGGTTCTACATG240195.018071659876737No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG169203.5349420244437484No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC147863.0891047738431006No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT132482.7677843936070197No Hit
TACTGAATGCAAAGGACACCACATTTAGCTGGGACACATAGACCAAGTCT118232.4700720777185836No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA105112.195967826177792No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA104192.1767471012221873No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTAAGG99882.08670218322365No Hit
GCCTACCAAAATCAGAGAAGCCATCCACTTGTAGAACCATGACCATCATC98822.064556565340019No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAAAGACCAAGTC83051.7350882690901495No Hit
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT69141.4444792645983495No Hit
TTCCCAATCACAGCCATTCGTCAGTGTACCCTCAATTTGGAAGCCTCTTA68151.423796093178732No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC66451.38827953619555No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTAAGGATTG66361.3863992478846756No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA65981.3784602527943173No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTATGG64961.357150318604408No Hit
AGAGGAACGTTACACACCAGCAAATGATCAGCCTTGCAAAGACCAAGTCT62821.3124412409902853No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC47290.9879870469027474No Hit
TGCAGATTTCATGTGCCTAGAAGTACACTGACTATTTTATTAAACTCTCA44160.9225947978690067No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC42320.884153347957798No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAAG42160.8808106131829103No Hit
GCCCTCTCAGAAACATCTGCTACCGTATTTACCCTACCAATTGCCCAGTG41740.87203593439883No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTAAGGA39070.8162540478428914No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTAAGG37080.7746787840802256No Hit
TGAACTTATATATTTGACGCAACCCATTCAGCAGAATTAGACCAAGTCTC34010.7105400605870678No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTT30160.6301055050663324No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTAAGGATT28540.5962603154705943No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTAAGGAT27190.5680559908074794No Hit
CTTAAATGTTGAAGGTGGTTCTTCAGCAAATTGGAAAGGTAAAGTAAAGA26990.5638775723388697No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTAAGGA26760.5590723910999686No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG24500.5118562624046797No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAA24170.5049618719314739No Hit
CTGGTGTTGTTGGGCAGTGTAGAGCGAGGTAATCATCTGCATTAGACCAA23740.4959782722239632No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTAAGGAT22330.4665204220202653No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG21570.4506424318395487No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT21510.4493889062989659No Hit
TTAACTACAATTCCCAGCAAGCGGTCTCTGAGTCGAGACCAAGTCTCTGC20360.4253630001044605No Hit
TGGGTCCAGTCAGTGAACGAGATACTTTCCTGAGTGCCAGACCAAGTCTC19940.4165883213203802No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTAAGGATT19750.4126188237752011No Hit
CAGTTAGCAGTTATAAATAGCCTGGGTTTTGATGTGTAACTTGTCAAGAC19410.4055155123785647No Hit
TGGCTTATAAAATATTAATGTGCTTCTGTTTCAAAAGACCAAGTCTCTGC17800.3718792437062572No Hit
TGTTTCAGGAAGGAATGTTCCCAATAGTAGACATAAAAGTCTTCGCACAG16860.35224067690379196No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTA16700.3488979421289042No Hit
TCTATTGTGATGGCCACGTTAGACCAAGTCTCTGCTACCGTAAGGATTGA14700.3071137574428079No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTAAGGATTGAGGATC14680.30669591559594694No Hit
AATCTCCAAGGAAGTTGTACCGTCTTAGACCAAGTCTCTGCTACCGTAAG14240.2975033949650058No Hit
GAGATTGATGACCAAAAGAACTGCTACCGTACTCATGGTGGGGGCAGCAG13900.2904000835683694No Hit
GTGCTGGTAAATTCACTCCATCGAATCATCACCAATAGACCAAGTCTCTG13290.27765590723911No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC12950.2705525958424736No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT12220.2553013684320485No Hit
TGAATTCTCCTCAGATGACTCCATTTAAAGACCAAGTCTCTGCTACCGTA12070.25216755458059126No Hit
AGCTGCTCACCATCGCTACCGTATGAGAAACACACTCAGCAAGTGAGACC11770.2458999268776768No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT11700.24443748041366345No Hit
TTGGTTGTTTGTCATCTTTCTGTACACTGACGACATGGTTCTACATCATG10860.22688812284550297No Hit
CACCCTTGAACAGTAAAAGCGAAGACCAAGTCTCTGCTACCGTAAGGATT10800.2256345973049201No Hit
CTCCCAATCACAGCCATTCGTACACTGACGACATGGTTCTTCACAAAGTG10210.2133082628225217No Hit
TCCCCTTTGCTTTGTCTTTTTCCAGCCTCCCCCACTGGCAGGATTGCCAT10040.20975660712420346No Hit
GCCCTCTCAGAAACATCTGCTTTGGTCTGTGACACTCATGGTGGGGGCAG9060.1892823566280163No Hit
AACAATCGACAGGCTAGAAGTTGAGACCAAGTCTCTGCTACCGTAAGGAT8660.18092551969079704No Hit
GAGTCACAGTCACAGGTAGGTTGTACGGTAGCAGAAAGTCAGTCCCATGG8400.1754935756816045No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC7720.16128695288833175No Hit
CCAAATCTGCTTTCTTGATAAAATCCTTTGAATGGCATGCAGACCAAGTC7340.15334795779797347No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCGAGACCAAGT7290.15230335318082106No Hit
GTGGGGTTCGCTCATTATTTCCTAGACCAAGTCTCTGCTACCGTAAGGAT6730.14060378146871408No Hit
CACAAAGGTTTTTATAAGTTCTGTGGATCTAGACCAAGTCTCTGCTACCG6700.13997701869842266No Hit
ATTTAACACCTAGCCAAAAGGCAGACCAAGTCTCTGCTACCGTAAGGATT6430.13433615376579963No Hit
TGATAAATGCACCTGTTTGCTGTCACATTCAGAGACCAAGTCTCTGCTAC6430.13433615376579963No Hit
GACTACAGATGTGCCACCATACCCAGAGACCAAGTCTCTGCTACCGTAAG6010.1255614749817194No Hit
CACTTAATGAGACCAACAGTAACACCACTGACTCTGATCCAGGAGACCAA5840.12200981928340124No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC5840.12200981928340124No Hit
TGGATCCTTTATTACGAATTGTGATCATTAAGACCAAGTCTCTGCTACCG5670.11845816358508304No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC4910.10258017340436644No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)