FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100574

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100574
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478650
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG386728.079389950903582No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA332246.941188760054319No Hit
TCTGGATCAGAGTCAGTGGTGTTACTGTTGGTCTCATTAAGTGTGTAGAA288626.029875692050559No Hit
AATTCTGCTGAATGGGTTGCGTCAAATATATAAGTTCATGTAGAACCATG266525.568160451269195No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC259945.430690483651937No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT165563.458894808315053No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG141142.948709913297817No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT130552.727462655384937No Hit
ATGTGTCCCAGCTAAATGTGGTGTCCTTTGCATTCAGTATGTAGAACCAT115812.4195132142484073No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT103452.161286952888332No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT102422.139768097774992No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA99332.0752115324349734No Hit
ACATGTTAGCTGACTGATGATGGTCATGGTTCTACAAGTGGATGGCTTCT94251.9690797033322887No Hit
TTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA81951.7121069675127962No Hit
AAGAGGCTTCCAAATTGAGGGTACACTGACGAATGGCTGTGATTGGGAAT67121.4022772380653923No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG66001.3788780946411783No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC65601.3705212577039592No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT65461.3675963647759324No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT64081.3387652773425258No Hit
TTGCAAGGCTGATCATTTGCTGGTGTGTAACGTTCCTCTTGTAGAACCAT61981.2948918834221248No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG46750.9767053170375012No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG41780.872871618092552No Hit
TGGGAGGTGAGAGTTTAATAAAATAGTCAGTGTACTTCTAGGCACATGAA41730.8718270134753996No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG41720.8716180925519691No Hit
CACTGGGCAATTGGTAGGGTAAATACGGTAGCAGATGTTTCTGAGAGGGC41320.8632612556147498No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT38980.8143737595320172No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA36690.7665308680664369No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAGGGGGCTAGAA28980.6054528361015356No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT28280.5908283714614019No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC27240.5691005954246318No Hit
TTACTTTACCTTTCCAATTTGCTGAAGAACCACCTTCAACATTTAAGTGT26750.5588634701765383No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT26530.5542672098610676No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA23900.49932100699885096No Hit
AATGCAGATGATTACCTCGCTCTACACTGCCCAACAACACCAGTGTAGAA23570.492426616525645No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT23380.4884571189804659No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC22020.4600438733939204No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT21020.43915178105087227No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT21010.4389428601274417No Hit
CGACTCAGAGACCGCTTGCTGGGAATTGTAGTTAATGTAGAACCATGTCG20230.4226470280998642No Hit
GGCACTCAGGAAAGTATCTCGTTCACTGACTGGACCCATGTAGAACCATG19880.41533479577979737No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT19400.40530659145513426No Hit
TGACAAGTTACACATCAAAACCCAGGCTATTTATAACTGCTAACTGTGTA19390.40509767053170376No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT16420.34304815627285073No Hit
AGCTTCATCATACCTTTCACTAAGACAAGAATTTAGAAGTGGACAGGAAA16030.33490024025906195No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT15540.32466311501096834No Hit
AACGTGGCCATCACAATAGATGTAGAACCATGTCGTCAGTGTAGATCTCG14630.3056513109787945No Hit
AAGACGGTACAACTTCCTTGGAGATTTGTAGAACCATGTCGTCAGTGTAG14100.294578502036979No Hit
GCTGCCCCCACCATGAGTACGGTAGCAGTTCTTTTGGTCATCAATCTCTG13640.2849681395591769No Hit
ATTGGTGATGATTCGATGGAGTGAATTTACCAGCACTGTAGAACCATGTC13160.27493993523451377No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA12920.2699258330721822No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT12190.25467460566175704No Hit
TTAAATGGAGTCATCTGAGGAGAATTCATGTAGAACCATGTCGTCAGTGT11850.24757129426512064No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG11590.24213935025592814No Hit
AAAAGCCCCTAAACCCCACTTCCCACTTTGTGAAGAACCATGTCGTCAGT11070.23127546223754308No Hit
CACTTGCTGAGTGTGTTTCTCATACGGTAGCGATGGTGAGCAGCTTGTAG10850.2266792019220725No Hit
TCGCTTTTACTGTTCAAGGGTGTGTAGAACCATGTCGTCAGTGTAGATCT10830.22626136007521153No Hit
AAGGGAATTGTTGAAGGACAGCATGATGTAGAACCATGTCGTCAGTGTAC10510.21957589052543613No Hit
CAGTACTTGTCCCTGAGGTAGTTTCTTTTCGAGAAGTTTTGGATGGAGGT9370.19575890525436124No Hit
GCTGCCCCCACCATGAGTGTCACAGACCAAAGCAGATGTTTCTGAGAGGG9060.1892823566280163No Hit
CAACTTCTAGCCTGTCGATTGTTTGTAGAACCATGTCGTCAGTGTAGATC8700.18176120338451895No Hit
CCATGGGACTGACTTTCTGCTACCGTACAACCTACCTGTGACTGTGACTC8260.17256868275357776No Hit
GCATGCCATTCAAAGGATTTTATCAAGAAAGCAGATTTGGTGTAGAACCA7230.15104982764023817No Hit
CGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC7150.1493784602527943No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG6930.14478219993732375No Hit
AGATCCACAGAACTTATAAAAACCTTTGTGTGTAGAACCATGTCGTCAGT6700.13997701869842266No Hit
AGGAAATAATGAGCGAACCCCACTGTAGAACCATGTCGTCAGTGTAGATC6460.1349629165360911No Hit
GCCTTTTGGCTAGGTGTTAAATTGTAGAACCATGTCGTCAGTGTAGATCT6330.13224694453149483No Hit
CTGAATGTGACAGCAAACAGGTGCATTTATCATGTAGAACCATGTCGTCA6290.13141126083777288No Hit
CTGGGTATGGTGGCACATCTGTAGTCTGTAGAACCATGTCGTCAGTGTAG5850.12221874020683172No Hit
CCTGGATCAGAGTCAGTGGTGTTACTGTTGGTCTCATTAAGTGTGTAGAA5810.1213830565131098No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG5770.12054737281938785No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)