FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715109
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC426805.968320913315313No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC334324.675091489549145No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG325834.556368329863No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT309294.325074918648766No Hit
ACCTTGTCACCAATACCTCACATACACTGACGACATGTTTCTTCTATAAC300504.202156594309399No Hit
AGACATCGCTGAATTTAAAGGATACAGATGAAATTGCTGCCACAGACCAA281033.9298904083153756No Hit
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT230383.221606776029948No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA211232.9538154323326933No Hit
TACTGAATGCAAAGGACACCACATTTAGCTGGGACACATAGACCAAGTCT202152.82684178216188No Hit
TGAACTTATATATTTGACACAACCCAGCAACCCATTCAGCAGAATTAGAC201732.8209685516473715No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA163292.2834281207480256No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC157712.2053980581981207No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA130981.831608887596157No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTA125181.7505023709672232No Hit
GATTCCCGCGCCCAGAGTCCCACGACCCCCTCAGACCAAGTCTCTGCTAC119611.6726121472390922No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTACGT115381.6134603256286806No Hit
CGACACATGACTGCAATGAAATTCAGTCCTGGAAGGAAAAGAAGAAGTAA112541.5737461002448576No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT104531.4617352040038651No Hit
TTGCATAAAAATTAACAGCAAAAATGCCTGGTAGAAGACTTCCTCCAGAC97741.3667846440193034No Hit
TCTTGCTTTTTTATTTCAGGATGCTTATGGTTCTACAAGCTGTGTCTACT90601.2669397252726506No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC85851.2005162849299897No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA85231.19184627798No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTACGTCAAG79351.1096210507768747No Hit
CCTATCTCATCGTCTTATATTGGCTTCACTGTCTTTCAACAGGCCTTGCT75841.0605376243341924No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC68170.9532812480335165No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTACG67690.9465689845883635No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTT59380.8303629236941501No Hit
CTGTGGTGCCACCTAAGCTCTGCTACCGTACAGCAAAAAGTCCTGCAACT58870.8232311437836749No Hit
TTCAACGTTGCCTCCTTTGCCCCTCCCACCCTTATTACTTGGAGATGATG56430.7891104712708134No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTACGTC52890.739607528362809No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC50830.7108007310773603No Hit
CTCCTCCACAGCTCGCTACACTGACGACATTCAGAGTGAAGAAATTTTAC48330.6758410256338544No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA41810.5846661138371912No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTACGT40840.5711017481251109No Hit
AACTGAGCGACCTCAGAGCCCTGGCAGCTCCAAGGCGCCTTTGCCAGCAG37860.5294297792364521No Hit
AAATAACCTAAGGGATTTGCTTTGTTTCAGACCAAGAGTGGTATGTGGGA36670.5127889594453433No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAC35170.49181313617923983No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCAGAGTATTGCCAATCATGTTC34780.4863594221300529No Hit
TCCAAATACTCCACACGCAAAGACATGGGGTTGGTGACAGACCAAGTCTC32720.4575526248446041No Hit
GGAAATTGAAGAAATCTGCCTAGTAGATGAGAACCAGTTCACCTTAACCA32420.4533574601913834No Hit
GGTGCCAGTCTTGCTCACAGGTGCCAGTTTGCTCACAGAAGGAGGACTCA31610.4420305156276875No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC31520.4407719662317213No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTACGTCA30870.43168244281640983No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTACGTCAA29470.41210500776804654No Hit
AAGTCCTCCATTTCTGTATCCATGGAGGAAGTAGGCAGCTGAGACCAAGT27650.3866543422051743No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAAAATGAAATACTTTGAGA26920.3764461082156706No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTACGTC26860.37560707528502646No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT26780.3744883647108343No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG26420.3694541671269695No Hit
CCAAATCTGCTTTCTTGATAAAATCCTTTGAATGGCATGCAGACCAAGTC26220.36665739069148895No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG25860.36162319310762414No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTACGTCA23020.3219089677238015No Hit
TCAGCTCACTTCAAGAAATCTCCATTACGGTAGCAGAAACTACCTGCTGC22620.31631541485284065No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGTTTGCTCACAGAAGGAGGACTCA22150.30974299022946156No Hit
TCTAAGCATAGCATTCAATTTTGGCCACTACAAATCATTCTCCTTAAGAC21450.2999542727052799No Hit
TTAACTACAATTCCCAGCAAGCGGTCTCTGAGTCGAGACCAAGTCTCTGC20970.2932420092601268No Hit
ATATTAACTAAATAGGAAAATACCAGCTTCATAGACAAAGGTTCTCTTTG20040.28023699883514264No Hit
GCTTTCACTTCTCTGGCTGAACACACAGCTCAGAATACTAGTAGACCAAG19870.2778597388649842No Hit
GTGGTGGTATACGATATGGGTTACATGGTTCTACAGTGGGGTTCGCTCAT19160.2679311825190286No Hit
GTGCTGGTAAATTCACTCCATCGAATCATCACCAATAGACCAAGTCTCTG18820.26317666257871175No Hit
GTAACTTGATTTGCTGTTGTATTTGCTTAGGAAGAGCAGCACTTCAGAAA17360.24276019459970438No Hit
AATCTCCAAGGAAGTTGTACCGTCTTAGACCAAGTCTCTGCTACCGTACG16950.23702680290696942No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC16710.2336706711843929No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAAAGACCAAGTC15900.22234372662069696No Hit
TGAACTTATATATTTGACACAACCCAGCACACTGACGACATGGTTCTACA15520.2170298513932841No Hit
TCTATTGTGATGGCCACGTTAGACCAAGTCTCTGCTACCGTACGTCAAGC15430.2157713019973179No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT14940.20891919973039078No Hit
TGAATTCTCCTCAGATGACTCCATTTAAAGACCAAGTCTCTGCTACCGTA14150.19787193281024293No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACCCGATATATGT13960.19521499519653646No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT13810.19311741286992612No Hit
ATACGGCCAGGCATTGAAGTCTTGTAGGACAGGGGCCAGGAGGAGACCAA13750.19227837993928198No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTACGTCAAGCATATC13550.18948160350380153No Hit
CGGACCCCATATAGCACAGGTACACTGACGACATGGTTCTACTCACTCAA12770.17857417540542772No Hit
GAATTGTGCAGAGTGTGGTGTACCATGAAGAATCCCCACCACAATACCAA11990.1676667473070539No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT10960.1532633486643295No Hit
TCACAATTGCCAGTTAACGTCTCTGCTACCGTAACGTTTCTGCTTTGCTG10600.14822915108046467No Hit
AACAATCGACAGGCTAGAAGTTGAGACCAAGTCTCTGCTACCGTACGTCA10180.1423559205659557No Hit
GCAGAGCCAAACCGACCTGAGCATCCCTCAAATGGCACAGCTGCTTAACA9810.13718188416031682No Hit
TGGCTTATAAAATATTAATGTGCTTCTGTTTCAAAAGACCAAGTCTCTGC9650.13494446301193244No Hit
CACTTAATGAGACCAACAGTAACACCACTGACTCTGATCCAGAAGACCAA9560.13368591361596624No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACGCGATATATGT9380.13116881482403384No Hit
GAAGAGACCAAGCAAGTTTTGAGACTCAGTCTGACAGAGTTCTCATTACC9310.13018994307161566No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCGAGACCAAGT8410.11760444911195357No Hit
TGATAAATGCACCTGTTTGCTGTCACATTCAGAGACCAAGTCTCTGCTAC8140.11382880092405494No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACCCAATATATGT7850.10977347509260825No Hit
CACAAAGGTTTTTATAAGTTCTGTGGATCTAGACCAAGTCTCTGCTACCG7530.10529863279583951No Hit
ATTTAACACCTAGCCAAAAGGCAGACCAAGTCTCTGCTACCGTACGTCAA7380.10320105046922917No Hit
CTGTATGTAGTCGGTGCTGTGAAAGCATCATCATCCAAGGAAGACCAAGT7240.10124330696439285No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)