FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences715109
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG423185.917699259833116No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA329224.6037736904443936No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC304944.264245031177065No Hit
TACTGGTGTGGGTTATAGAAGAAACATGTCGTCAGTGTATGTGAGGTATT290094.056584380842641No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT287704.023162902438649No Hit
GTGGCAGCAATTTCATCTGTATCCTTTAAATTCAGCGATGTCTTGTAGAA278833.8991258675250906No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT216313.024853553793897No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT208462.915080078701289No Hit
ATGTGTCCCAGCTAAATGTGGTGTCCTTTGCATTCAGTATGTAGAACCAT198852.7806949709764526No Hit
AATTCTGCTGAATGGGTTGCTGGGTTGTGTCAAATATATAAGTTCATGTA197482.7615370523934115No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT161492.2582571328287013No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG151692.121215087490159No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT129911.8166461336663362No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT126971.7755335200647735No Hit
GAGGGGGTCGTGGGACTCTGGGCGCGGGAATCTGTAGAACCATGTCGTCA118401.6556916498044354No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA115931.6211514608262516No Hit
GGATCCGTGGCGAGTTCGTCCACTGTTACAGTGGCCAGTGGTAGGGGAGA111581.5603215733545517No Hit
CCAACTGGCCAAGACCTACGGTAGCGATGGTGAGCAGCTTGTAGAACCAT103871.4525058417667798No Hit
GGAGGAAGTCTTCTACCAGGCATTTTTGCTGTTAATTTTTATGCAATGTA96781.353360117128997No Hit
GTTCAAATGAGTAGACACAGCTTGTAGAACCATAAGCATCCTGAAATAAA90231.261765688867012No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG85051.1893291791880678No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT84761.1852738533566212No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC78631.0995526556091448No Hit
CAGCTGTGCCATTTGAGGGATGCTCAGGTCGGTTTGGCTCTGCAGCAGGT75111.0503293903446886No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG67890.9493657610238438No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG67100.9383184941036962No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAGGGGGCTAGAA57220.800157738190961No Hit
ACAAGTTGCAGGACTTTTTGCTGTACGGTAGCAGAGCTTAGGTGGCACCA55670.7784827208159875No Hit
CATGAGGGTAGTGTTGACAAAGATTAAAACAGAAAATGCTTTATGTTTAG54570.763100450420845No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT53070.7421246271547414No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG50240.7025502405926929No Hit
GTTGTAAAATTTCTTCACTCTGAATGTCGTCAGTGTAGCGAGCTGTGGAG47740.6675905351491871No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA40540.5669065834718904No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC38990.5452315660969167No Hit
CACTGGGCAATTGGTAGGGTAAATCCACAGGGCGCCCGCGGAGGTGCAGA36680.5129287982671173No Hit
GAAACAAACTCCCACATACCACTCTTGGTCTGAAACAAAGCAAATCCCTT35610.4979660443372968No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA35060.4902749091397256No Hit
CTGAGTAAGTGGCAAGAAAATTACCTGCGTGCTGCATCAAAGTTGCTTTT32690.45713310837928206No Hit
GTCACCAACCCCATGTCTTTGCGTGTGGAGTATTTGGATGTAGAACCATG32600.45587455898331586No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA31310.4378353509744668No Hit
GAGTCCTCCTTCTGTGAGCAAACTGGCACCTGTGAGCAAGACTGGCACCT31240.4368564792220486No Hit
CTGTGACAGTTCCCAGCGGGTCCGGATATGAATGATAGACTGGACAATGG31190.4361572851131786No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC30750.4300043769551215No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT28970.4051130666793454No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTGATGTCTACAATATCACC27710.38749337513581844No Hit
CAGCTGCCTACTTCCTCCATGGATACAGAAATGGAGGACTTTGTAGAACC27460.38399740459146786No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTAAGAACACAGAGGAGAATTTATT26630.37239078238422396No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT26610.37211110474067594No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT26600.3719712659189019No Hit
GCATGCCATTCAAAGGATTTTATCAAGAAAGCAGATTTGGTGTAGAACCA26060.3644199695431046No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT25850.3614833542858501No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT25510.3567288343455333No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC22690.31729428660525877No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTATGGGATTCCATT22310.3119804113778459No Hit
GGTGGCAGCAGGTAGTTTCTGCTACCGTAATGGAGATTTCTTGAAGTGAG22280.3115608949125238No Hit
GAGTCCTCCTTCTGTGAGCAAACCTCCTTCTGTGAGCAAGACTGGCACCT21920.306526697328659No Hit
TAAGGAGAATGATTTGTAGTGGCCAAAATTGAATGCTATGCTTAGATGTA21090.29492007512141505No Hit
CGACTCAGAGACCGCTTGCTGGGAATTGTAGTTAATGTAGAACCATGTCG20870.29184362104238654No Hit
ACTAGTATTCTGAGCTGTGTGTTCAGCCAGAGAAGTGAAAGCTGTAGAAC19760.27632151182547No Hit
AGGAAATAATGAGCGAACCCCACTGTAGAACCATGTAACCCATATCGTAT18560.25954085321258713No Hit
ATTGGTGATGATTCGATGGAGTGAATTTACCAGCACTGTAGAACCATGTC18330.25632456031178463No Hit
TTATTTTTTGGGGGGAAATTTTTTAGGATCTGATTCTTCTGAAGATACCG18060.252548912123886No Hit
AAGACGGTACAACTTCCTTGGAGATTTGTAGAACCATGTCGTCAGTGTAG16750.23423002647148897No Hit
AAGAGGCTTCCAAATTGAGGGTGGGCGCCTGGGAGGGGTGTTTCTGTTGC16600.2321324441448786No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG16280.22765760184810988No Hit
TTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA15640.2187079172545724No Hit
AACGTGGCCATCACAATAGATGTAGAACCATGTCGTCAGTGTAGATCTCG15340.21451275260135166No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG14950.2090590385521648No Hit
TTCTTTGTAAGGCGTGTCTCAATGTAGAACCATGTCGTCAGTGTGCTGGG14940.20891919973039078No Hit
TTAAATGGAGTCATCTGAGGAGAATTCATGTAGAACCATGTCGTCAGTGT13940.19493531755298843No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT13870.19395644580057025No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT13810.19311741286992612No Hit
CCTCCTGGCCCCTGTCCTACAAGACTTCAATGCCTGGCCGTATTGTAGAA13590.1900409587908976No Hit
GCAGATGTCTTTGAGTGAGTAGAACCATGTCGTCAGTGTACCTGTGCTAT12630.1766164319005914No Hit
GTAGTCAGTGCATTCTACAACAGCGCGCATGTTAGCAAGTTCATCAACCA11690.1634715826538332No Hit
GTTGATACAAAACAAGCCCACGAACTGACCAAGAGTTACGGGATTCCATT11630.16263254972318905No Hit
CCACACAGCAAAGCAGAAACGTTACGGTAGCAGAGACGTTAACTGGCAAT10520.14711044050627248No Hit
CAACTTCTAGCCTGTCGATTGTTTGTAGAACCATGTCGTCAGTGTAGATC9990.13969898295224922No Hit
GGTCTCTGAGTCCTGCTGCTGGGAAGTAGCTTCCGTCAGGGCACTGATGG9740.13620301240789867No Hit
TCTGGATCAGAGTCAGTGGTGTTACTGTTGGTCTCATTAAGTGTGTAGAA9460.132287525398226No Hit
TAGGAGCCAGGAGAGAATGACCTGTCCCGGTAACTGGAACGGAAGGCAAT9190.12851187721032736No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA8520.11914267615146781No Hit
CGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC8210.11480767267647309No Hit
CTGAATGTGACAGCAAACAGGTGCATTTATCATGTAGAACCATGTCGTCA7970.11145154095389655No Hit
GCCTTTTGGCTAGGTGTTAAATTGTAGAACCATGTCGTCAGTGTAGATCT7360.10292137282568112No Hit
AGATCCACAGAACTTATAAAAACCTTTGTGTGTAGAACCATGTCGTCAGT7300.10208233989503698No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTGATATCTACAATATCACC7160.10012459639020066No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)