FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100579

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100579
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences847990
Sequences flagged as poor quality0
Sequence length150
%GC44

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC332663.922923619382304No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC284763.358058467670609No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC264233.1159565560914633No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT240522.8363542022901216No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG227372.681281618887015No Hit
GTATAATGATGAGCATTTTGGCTACCGTACACATAGGTTAACATTTACCA225262.6563992499911553No Hit
TGACAATGAGATTCATCAGTTTAACAACACTGACGACATGGTGGTGGTAA179302.1144117265533793No Hit
GGTCCAGCAGCCCTTTCCTGAGCAAGCGGTCTCTGAGTCGGAGTCCACTC175402.068420618167667No Hit
TACTGAATGCAAAGGACACCACATTTAGCTGGGACACATAGACCAAGTCT174912.062642248139719No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT173792.049434545218694No Hit
TCAGATATAAAAGAAGAGGTCTTGGCTACCGTACAAACCTAGTCATGATT172532.034575879432541No Hit
GCACGGTTTCTGTAGCCCATAGGTTGATAGAGTGGTAGACCTTGCAGACC165611.9529711435276358No Hit
CCGGTACAAACCTTTCATTGTATTGTCGTTTTAGTTCAACCAGACCAAGT161211.9010837391950377No Hit
CTGATGTTGAATTAACCAAAAATATTCCCTCTGCGTGTTCTCATAAGACC145631.7173551574900647No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA142721.6830387150791875No Hit
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATAGAAGCCAT125581.4809136900199293No Hit
CCTATCTCATCGTCTTATATTGGCTTCACTGTCTTTCAACAGGCCTTGCT124191.4645219872875859No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTT122151.4404650998242903No Hit
TTAACTACAATTCCCAGCAAGCCGCGGAGCCAACCTGGGCCTCTGCCGGC100941.1903442257573793No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA98991.1673486715645232No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTATTG89391.0541397893843087No Hit
TCGAATCAAGCACTAATACCAAGAGAAAAGGTAATTAAATGTGGTACGTG89291.0529605301949316No Hit
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT80940.9544923878819325No Hit
GGAAATTGAAGAAATCTGCCTAGTAGATGAGAACCAGTTCACCTTAACCA76960.9075578721447187No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACAAAATGAAATA76400.9009540206842063No Hit
TATGTCATGATTCATCTTACTAGCCTCAAACATATCTTCTTTGCCAGGAC66760.7872734348282409No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTATTGTCGA66000.778311064988974No Hit
ACAATAGTGGCCAAAAATGCTCATTGATAATAACAGTTTACATTTGTTTT64720.7632165473649454No Hit
GGGATCGATGAAGGACCGGATATCGGGAAGTTCAAAGCGTTCGAATGAGG64460.7601504734725645No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCAGAGTATTGCCAATCATGTTC64180.7568485477423083No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC63920.7537824738499275No Hit
GAATTGTGCAGAGTGTGGTGTACCATGAAGAATCCCCACCACAATACCAA63420.7478861779030412No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA62360.7353860304956427No Hit
TCGGGGGCCTACAAGAGTCTGACAGAGTTCTCATTACCCAAGACCAAGTC60560.7141593650868525No Hit
GAAGAGACCAAGCAAGTTTTGAGACTCAGTCTGACAGAGTTCTCATTACC59760.7047252915718346No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA58870.6942298847863771No Hit
AATTTGCATCTGTTTGTCCACATTAGGCTTAGGTTACCACAAGGATCTCC53060.6257149258835599No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCAAC52940.6242998148563073No Hit
TGTCATGATTCTGTTGTTTCAATGTACACTGACGACATGGTATTCGATGA52850.6232384815858677No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTATT52540.6195827780987984No Hit
GGGGACGCTCTTGTATTATCTGTACACTGACTTATGGTATCAAATAATAA51350.6055495937452092No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC49820.5875069281477376No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC49750.5866814467151735No Hit
AGGCTGGGTTTGCACTGATGTTGAATTAACCAAAAATGTTCCCTCTGCGT49240.5806672248493496No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTATTGT47020.554487670845175No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC44380.5233552282456161No Hit
ATCTGGTGTTGAAAATTCTAATGACTTTGCATTTTTGAAGGTTTTTCCTT43460.5125060437033455No Hit
CCTCTCTCCTTCTTCCCCAAGTACACACACAAACCTGCCAATGCCAGTGG43450.5123881177844078No Hit
GGATACAGTAGCCAGAATGACGACTCAATCAGTGCTTCGGTTGTTGCTTT43410.511916414108657No Hit
TTCTGATGAGCCAAGTCTCTGTGGGAGGAGGGCAGTACACAGACCAAGTC42260.4983549334308187No Hit
GTAACTTGATTTGCTGTTGTATTTGCTTAGGAAGAGCAGCACTTCAGAAA41760.4924586374839326No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG41320.4872698970506728No Hit
TTTTGTGCGAAGGAAACCGATGGGGATCGGAACCGTAGCGGTTGAGCTGC39940.47099612023726695No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC39910.4706423424804537No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA37950.44752886236865996No Hit
CCAGTGCAACAGGTGGCTTGGGATCAATAAAAGCTGAGGTGATGGCAGAT37240.43915612212408167No Hit
CCAGAGACCAGACTCCAGAGCCCAGACTCCCACATCTCCTTACCCTAGAC35450.4180473826342292No Hit
ACGCCTTCTGTCTGAGCACCCAGAATTAGAACATATCATCTGGACCCTTT35170.41474545690397296No Hit
TATTAACTAAATAGGAAAATACCAGCTTCATAGACAAAGGTTCTCTTTGA34500.40684442033514545No Hit
CTACTCTTTAGCTTCCTACCTAAGAATAAAAATGGGATTGTTTGCACTAA33910.3998867911178198No Hit
GGTCTCTCATGGCACTGTACTCTTCTTGTCCAGCTGTATCCAGTATGTCC33090.3902168657649265No Hit
GTTAACTGCTACTAAAACGGAGCTGTTTGGAAGCACCACTTACATTTGCA32190.37960353306053135No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACCCAATATATGT30820.36344768216606327No Hit
TATTTAGTAGCCAGGACAGTAGAAGGACTGAAGAGTGAGAGGAGCTCCCA29520.34811731270415924No Hit
AGAGGAACGTTACACACCAGCAAATGATCAGCCTTGCAAAGACCAAGTCT29190.3442257573792144No Hit
ACCTTGTCACCAATACCTCACATACACTGACGACAGGGTTCTATGATTTC27870.3286595360794349No Hit
TGACACACTTGCAGAAACAGTATTGGCTGATCGGTTTGAGAGATTGGTGG27850.32842368424155943No Hit
CCCAAGATTACTGGCATCTTCCTGGGGCTGGCTCACCTGTCGGGAAGATT27700.3266547954574936No Hit
CTCACGTAATTTTGTACTTTTTCTTCCTATTGGTCTTTGTTTTTCTCTAG27470.323942499321926No Hit
TCAAATCATCCATTGCTTGGTACGGTAGCAGAAAGTCAGTCCCATGGAGA27410.32323494380829965No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTATTGTC27260.32146605502423375No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTATTGT26870.3168669441856626No Hit
TGAAGAAGGAGATGGTGTGGAATTGATGGAAGCCAAATCACAGTTATTTC26440.31179612967134046No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAT26260.30967346313046146No Hit
CTCAGTCACACATGCCAGAGATTGCTCAGGAAGCAATGGAGGTAAGGAGA26090.3076687225085202No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAAAATGTTAGTACTGTTTGAA24940.294107241830682No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTATTGTCG24850.2930459085602425No Hit
TGACAGTTTGACAGTTAAAGGCATTACGGTAGCAGCATCTTGAATCTCAT23990.28290427953159825No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTATTGTCG23780.2804278352339061No Hit
GGCCTGCCCAGGTTGTGCGAGGCCCGCGCGGCTCCCTGGCACGCGCACAC22670.2673380582318188No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAAAGACCAAGTC21980.2592011698251159No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT21900.2582577624736141No Hit
GGAAACACTGATGGGCCTGAAACAGAGTTCAGTGCCATTGACACTGATGA21510.25365865163504286No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTATTG20350.23997924503826695No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC20080.23679524522694845No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAAA20020.2360876897133221No Hit
TGAACTTATATATTTGACACAACCCAGCAGTTCGTAAGTAGTTCACAGAA19710.23243198622625266No Hit
CCAGACTCTCCAGAACCAAAGGTACGGTAGCAGCATCTTGAATCTCATAC19160.2259460606846779No Hit
TCGGGGGCCTACAAGAGTCCCACCAAGAGGGGGAAGAGGGAGAGGAGTTC18890.22276206087335934No Hit
TCCTCCCCTCTTAGCTGTCTTAAAGAATGGCAGACTGACAGTTGGTCAGA18850.22229035719760845No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG18780.22146487576504442No Hit
TTTTGTGTTTGCACAGTGCCTTTCAGAATGTCCCACCCATAGAGTAGCAG18780.22146487576504442No Hit
CCAGACTCTCCAGAACCAAAGGCAATCACACCACCTCAGCAACCATATAA18740.22099317208929353No Hit
CCATTTTCTTCACACCTGTCGTGAAGGAAACCAGCATGCAGCTGAACTTC18710.22063939433248034No Hit
TTAACTACAATTCCCAGCAAGCGGTCTCTGAGTCGAGACCAAGTCTCTGC18580.21910635738628995No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG18000.212266654087902No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG17980.21203080225002655No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTATTGTC17800.20990813570914754No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTATTGTCGAGACATC17580.20731376549251757No Hit
TGTGATTATTTCTATTTTAGCAACCAAAGGACACAATGAGATTAGATGAA17350.20460146935694998No Hit
GTGCTGGTAAATTCACTCCATCGAATCATCACCAATAGACCAAGTCTCTG16660.19646458095024705No Hit
CCTTCCGTTCCAGTTACCGGGACAGGTCATTCTCTCCTGGCTCCTATGAG16590.195639099517683No Hit
CAGCAGTTACCAGGGCACAGGGTCAGTGCAGTTTCCAGGGGACCAGGACC14830.1748841377846437No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT14710.173469026757391No Hit
TCAATGGCAAGTAAGTTATTTATGGTCGTTTTTAAGGATAAGCTGTTAAC14570.17181806389226287No Hit
TTTGCAGACCTTCTCAGGAGTCACTACTTCATTCCTGCTCTTAGTGTCTG14540.1714642861354497No Hit
TTCCCAATCACAGCCATTCGTGAAATCAAAATCCTTCGTCAGTTAATCCA14300.16863406408094433No Hit
TCTATTGTGATGGCCACGTTAGACCAAGTCTCTGCTACCGTATTGTCGAG13940.1643887309991863No Hit
AGCAAGCTGTGACGGGTAAGCTTTATAAGGAAAGTAAATCTTCACCCCAC13610.16049717567424143No Hit
GCTTTCAAGTGATAATTGCCTTCATTTTAGGCTTGGGAAGATACACATGC13560.15990754607955282No Hit
AATCTCCAAGGAAGTTGTACCGTCTTAGACCAAGTCTCTGCTACCGTATT13530.15955376832273965No Hit
AGTCTTCAGAATGCCAGAGATATACAGGATATGCGAATTAAGAAGAAACA13480.15896413872805104No Hit
CCACTGTAAGTTTATGTCATGGTTGTTTTTTATATTTCAGTCGCCCTTAC12900.15212443542966309No Hit
GTTAGAAGGCTGGCTCCCATGCTGTTCTAACACAGCTTCTAGTTCAGCCA12790.15082725032134814No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT12480.14717154683427872No Hit
TTCTGTAGTCCAGATTCTGATGATGCTACAAGTGGAACTGTTATTATCCC12410.14634606540171463No Hit
TTAACTACAATTCCCAGCAAGCCGCGGAGCCAACCTAGGCCTCTGCCGGC12330.14540265805021285No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT12330.14540265805021285No Hit
CCAAATCTGCTTTCTTGATAAAATCCTTTGAATGGCATGCAGACCAAGTC11680.13773747331926084No Hit
GGCAGTTCTCTTGAGTCAGCTGATGAAAACCCAAGAGCCAGCAGGTAGTC11600.13679406596775906No Hit
TGAATTCTCCTCAGATGACTCCATTTAAAGACCAAGTCTCTGCTACCGTA11150.1314873996155615No Hit
TCCCCTTTGCTTTGTCTTTTTCCAGCCTCCCCCACTGGCAGGATTGCCAT10770.12700621469592802No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACCTGATATATGT10550.12441184447929811No Hit
ATTTGTTGCAGGTTTTGGTTTTAATGGCTTGTGGCGGTTTGCAGGACCGT10460.1233505112088586No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA9390.11073243788252221No Hit
GAAGTTCATCCTGTTTTAAGTCACACTTGTGATTTGTTAAATTTTTTAAC9200.10849184542270547No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)