FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences847990
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG327103.8573568084529297No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG305213.5992169718982536No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA280313.3055814337433223No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC237712.8032170190686214No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT224462.6469651764761375No Hit
AGGGGTGGTAAATGTTAACCTATGTGTACGGTAGCCAAAATGCTCATCAT212902.5106428141841297No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT193992.287644901472895No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT171772.0256135095932737No Hit
ATGTGTCCCAGCTAAATGTGGTGTCCTTTGCATTCAGTATGTAGAACCAT171482.02219365794408No Hit
AGGGCTTTAAAATTACCACCACCATGTCGTCAGTGTTGTTAAACTGATGA170972.016179436078256No Hit
GCCTCTAGAAATCATGACTAGGTTTGTACGGTAGCCAAGACCTCTTCTTT170092.005801955211736No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT164921.9448342551209328No Hit
GCAAGGTCTACCACTCTATCAACCTATGGGCTACAGAAACCGTGCTGTAG163971.9336312928218493No Hit
GGTTGAACTAAAACGACAATACAATGAAAGGTTTGTACCGGTGTAGAACC159221.8776164813264309No Hit
CAATGATGAACACTGCGGGCTTCCAGACAATGTTAACACGCTGAATGCCA155481.8335121876437221No Hit
TATGAGAACACGCAGAGGGAATATTTTTGGTTAATTCAACATCAGTGTAG143321.6901142702154506No Hit
CAGCTGTGCCATTTGAGGGATGCTCAGGTCGGTTTGGCTCTGCAGCAGGT123101.451668062123374No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAGGGGGCTAGAA118241.3943560655196405No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT97731.15249000577837No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA88911.048479345275298No Hit
TGCACGTACCACATTTAATTACCTTTTCTCTTGGTATTAGTGCTTGATTC85871.0126298659182302No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTGTCTGTCTAAGAACACAGAGGAG75330.8883359473578698No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC65540.7728864727178386No Hit
GTGGATTAACTGACGAAGGATTTTGATTTCACGAATGGCTGTGATTGGGA63880.7533107701741766No Hit
TCTAAAGTATGCAGGTTTTGTTCAAGAAGTGCGGTACCTGCTGAATACAA63630.7503626222007335No Hit
ACTTTCAAGTTTCCCCACCCTTTACACAGTTTCCCCGCGGAAAACGCCGC63360.747178622389415No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG63220.7455276595242868No Hit
CTGAGTAAGTGGCAAGAAAATTACCTGCGTGCTGCATCAAAGTTGCTTTT62150.7329095861979504No Hit
GGTCTTTGTGCCCAGACTTCAGCTCCCGTTCTTTCCTGGTCTTCTCCTTG61960.7306689937381338No Hit
CTGTGACAGTTCCCAGCGGGTCCGGATATGAATGATAGACTGGACAATGG61830.7291359567919433No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT61400.7240651422776212No Hit
TGGGTAATGAGAACTCTGTCAGACTCTTGTAGGCCCCCGATGTAGAACCA59910.7064941803559004No Hit
GTAGTCAGTGCATTCTACAACAGCGCGTATGTTAGCAAGTTCATCAACCA58480.6896307739478059No Hit
TAGGAGCCAGGAGAGAATGACCTGTCCCGGTAACTGGAACGGAAGGCAAT58310.6876260333258647No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT58230.6866826259743629No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG52590.6201724076934869No Hit
CAGTCAAGAGTCATCGAATACCATGTCGTCAGTGTACATTGAAACAACAG52570.6199365558556115No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTACAGATAATACAAG50520.5957617424733782No Hit
TCCACCAATCTCTCAAACCGATCAGCCAATACTGTTTCTGCAAGTGTGTC49570.5845587801742945No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG49310.5814927062819137No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG49270.5810210026061629No Hit
TATGAGAACACGCAGAGGGAACATTTTTGGTTAATTCAACATCAGTGCAA48100.5672236700904493No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT46550.5489451526551021No Hit
GCAGTCTGGGGCCCTTTGGCAGGCCCTGGTTGCCCTGAGGCGGGTGCTGC43690.5152183398389132No Hit
GTAGACGATGTAAAGCAAGCACATTGCCGTCACTTATGAAGGAAAGACTA43090.5081427847026498No Hit
GTGTACTGCCCTCCTCCCACAGAGACTTGGCTCATCAGAATGTAGAACCA41750.4923407115649949No Hit
ACTTTCAAGTTTCCCCACCCTTTACACAGTTTCCCCGTGGAAAACGCCGC41710.49186900788924404No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA41690.49163315605136854No Hit
AAGAGGCTTCCAAATTGAGGGTGGGCGCCTGGGAGGGGTGTTTCTGTTGC40550.478189601292468No Hit
AGGAAATAATGAGCGAACCCCACTTCCCCACAACGGCACCCCACATGAAA40340.47571315699477584No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG39170.46191582447906226No Hit
CTGAGGTCGCTCAGTTCCGGAGCTGCAACCCGGAGTCCCAGATGATCGGG38790.45743463955942876No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG37580.44316560336796423No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTGATGTCTACAATATCACC36150.42630219695986976No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC35920.4235899008243022No Hit
AACAGATATGCTATAGTACAGAAGGCAAAAGAAAAGTGATTACCTTGCTT35390.4173398271206028No Hit
AGGGTAAGGAGATGTGGGAGTCTGGGCTCTGGAGTCTGGTCTCTGGTGTA34740.40967464238965084No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTGCTTGATTTTCTTACTTGGTCCAA33450.39446219884668454No Hit
GGCACTTTTCTTCCCCGCTTACTCTAATCACTTACCCACTGAGAACAAGG32880.3877404214672343No Hit
CTGCCCCCTTACCCTCCACACCCCCAGGATTCTTACAGAAAACAAGTGGT32820.387032865953608No Hit
AACATGTTCCAGAGCAGTTGGTAGAGTAAATGCCGAGAATCAAACAGAGT32760.38632531043998164No Hit
GAAAACTTTTATTGATTTATTTTTTGGGGGGAAATTTTTTAGGATCTGAT32290.3807827922499086No Hit
TGCAAATGTAAGTGGTGCTTCCAAACAGCTCCGTTTTAGTAGCAGTTAAC31510.37158457057276617No Hit
CTACCAGTGCCAGGAGCTGGACACCTACCTGATACCCCAGATCCCCCACA29520.34811731270415924No Hit
TTGCAAGGCTGATCATTTGCTGGTGTGTAACGTTCCTCTTGTAGAACCAT28510.3362067948914492No Hit
AAGACATCACTGAAATCATAGAACCCTGTCGTCAGTGTATGTGAGGTATT27910.3291312397551858No Hit
CACAACCTTGCACTGCTTTATGAAGTGTTTCACCAAACATATTTCGAAGT27420.3233528697272373No Hit
CCATGGGACTGACTTTCTGCTACCGTACCAAGCAATGGATGATTTGATGT27190.3206405735916697No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC26980.3181641292939775No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT26800.31604146275309847No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA26040.3070790929138315No Hit
CAGAAATGACATCTACCTGTTACTCACAGAAACCACACACCAAAGGAACA25550.3013007228858831No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT24570.2897439828299862No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT23560.2778334650172761No Hit
CCTGTCATGGGTATTATTTCTTTGCTTTTTTTGTGTGGTGGGGGGCTTTA23550.2777155390983384No Hit
GTATGAGATTCAAGATGCTGCTACCGTAATGCCTTTAACTGTCAAACTGT23520.27736176134152524No Hit
TTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA21850.2576681328789254No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT21500.25354072571610514No Hit
GGACCCCCCGCGAATGCCCACGTGACGTGCAGTCCCCCTGGGGCTGTTCC21420.25259731836460336No Hit
TCCCCTGGAAACTGCACTGACCCTGTGCCCTGGTAACTGCTGGACTCCCC20490.24163020790339507No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA20290.23927168952464062No Hit
TATGAAGTAGCCTGCTATAATCGATCAAACTAACCCTAACTATCAAGATG19430.2291300604959964No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC19160.2259460606846779No Hit
GTATGAGATTCAAGATGCTGCTACCGTACCTTTGGTTCTGGAGAGTCTGG18700.22052146841354264No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT18460.21769124635903728No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT18370.21662991308859775No Hit
CGACTCAGAGACCGCTTGCTGGGAATTGTAGTTAATGTAGAACCATGTCG18370.21662991308859775No Hit
AATGCAGATGATTACCTCGCTCTGGCAAATGATTTCATTAAAGATTCAGA18310.2159223575749714No Hit
TAACATAAGAGATTCTGGGGCTTCAAGAGGTGTACAGGCATCAGGAGAGC18270.2154506538992205No Hit
TACTGGTGTGGGTTATAGAAGAAACATGAGAGATTTAGTTCAAAAGGAAG18120.2136817651151547No Hit
TGGATTTACACACTAACCTGGTAGAAATGCGACTAAAGACTGCATTGAAG18070.21309213552046605No Hit
AAAGAGAGTGAGTCGTTGCGAAGCCCTGTGTTTCAGCTCATTGTGATTAA17730.20908265427658346No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC17700.20872887651977026No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT17610.20766754324933076No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG17410.2053090248705763No Hit
GCAGAATTCCGAAGTTCAGCTGCATGCTGGTTTCCTTCACGACAGGTGTG16950.19988443259944103No Hit
ATTGGTGATGATTCGATGGAGTGAATTTACCAGCACTGTAGAACCATGTC16360.19292680338211535No Hit
CAATGATGAACACTGCGGGCTTCCAGACAATGTTAACATGCTGAATGCCA15980.18844561846248187No Hit
CTTAGTTTCTTATCATTTTGGCTTCTATACCTCCATGATCTCCAACAAAG15920.18773806294885553No Hit
CCAGTCCTCAGATAAATGGTGAAATAGACTTACTAGCAAGTGGCTCCTTG14780.17429450818995507No Hit
TGCAATTTGGTCTCTGCATGTACCACAGAATTGCTGCTTCCCTCAGCCTT14270.16828028632413117No Hit
ATTGGACCAGTCTACCCTGTAAAAATGTGATTCTTTGCTAGTACCTTTGT13880.16368117548555997No Hit
AACGTGGCCATCACAATAGATGTAGAACCATGTCGTCAGTGTAGATCTCG13690.1614405830257432No Hit
CTGTGACAGTTCCCAGCGGGTCCGAATATGAATGATAGACTGGACAATGG13600.1603792497553037No Hit
GCAGGTGACCAACCACATAACTCGAGACAGGGACAGCGGGAGGCTCAAAC13590.160261323836366No Hit
AAGACGGTACAACTTCCTTGGAGATTTGTAGAACCATGTCGTCAGTGTAG13540.15967169424167738No Hit
AAGTACATATATTTAGCAGATCAGTTAACAGACAAAAGTCAACTTTACAG13180.15542636115991934No Hit
GCAGGTGACCAACCACATAACTCGAGACAGGGACAGCGGGAGACTCAAAC12910.1522423613486008No Hit
TTCTTTGTAAGGCGTGTCTCAATATTTGACATTTTCATTTCTGTTGTAGT12890.15200650951072536No Hit
TATGAGAACACGCAGAGGGAACTTGGCCTCCTACTGCTGCTTTCAGAGAG12870.15177065767284992No Hit
CATTCCTTGTTGTGCTCAGTACTGACTTTGGTATGTGTGTTCTGCTGTCC12560.1481149541857805No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT12380.14599228764490146No Hit
GCACCTGAGCTCAATGAGATTCATGACCGGTCTGTTTACCTGCTGCCTCC12350.1456385098880883No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTCTTGGTGCCATTTATCGTTTTTG12270.14469510253658652No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG12170.1435158433472093No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGCATCATCAGAATCTGG11910.14044976945482848No Hit
GCATGCCATTCAAAGGATTTTATCAAGAAAGCAGATTTGGTGTAGAACCA11790.13903465842757579No Hit
TTACCCACAGCTCCAAACATCTATGGCTGGTGTGTAACGTTCCTCTCCTA11600.13679406596775906No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA11200.13207702921025014No Hit
TTAAATGGAGTCATCTGAGGAGAATTCATGTAGAACCATGTCGTCAGTGT10970.1293647330746825No Hit
CAGTACTTGTCCCTGAGGTAGTTTCTTTTCGAGAAGTTTTGGATGGAGGT10420.1228788075331077No Hit
ATTGTGTGTTCTTAAAGCAGGCATACTAATTTGAACAGAAACCTTCATTT10110.11922310404603828No Hit
GGCATTCCTTTTTGAACAGTACCCGTTCCCTTGATGTCTACAATTTCACC9600.1132088821802144No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT9220.10872769726058092No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTAGGCTGCAGCGACTTCGAACT9120.10754843807120368No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)