FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1088907
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC221982.0385579301078973No Hit
GTTGTTTCAGAACACCGAAAAGCAAACTAAGTATCAAGTGAGCAAGACCA196601.805480174156287No Hit
TACTGAATGCAAAGGACACCACATTTAGCTGGGACACATAGACCAAGTCT189021.7358690870753886No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC181961.6710334307704882No Hit
TTAACTACAATTCCCAGCAAGCGGTCTCTGAGTCGAGACCAAGTCTCTGC177621.6311769508323484No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT166711.5309847397436145No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCACCACTGACTCTGATCCAG164621.5117911814323906No Hit
CCAAATCTGCTTTCTTGATAAAATCCTTTGAATGGCATGCAGACCAAGTC164131.5072912562780845No Hit
TGATAAATGCACCTGTTTGCTGTACACTGACGACATGTTTCTTCTATAAC160261.4717510310797892No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA157611.447414701163644No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTA154971.4231702064547294No Hit
CCTCAGGGCCTCAGAGAAAGGGTGGGGAAACTTGAAAGTAGACCAAGTCT151921.3951604682493546No Hit
GCTTCCAGTACTTCAACTTTGCCACACTGACACAATGGCAACAGTTCTGA148341.3622834640607508No Hit
CCGGTACAAACCTTTCATTGTATTGTCGTTTTAGTTCAACCAGACCAAGT146621.346487808417064No Hit
CTGTGATACTGAGAAAAGACAGTAGTACACTGACTTATGGTATCAAATAA141951.30360076664031No Hit
GGGGACGCTCTTGTATTATCTGTACACTGACTTATGGTATCAAATAATAA140851.2934988938449288No Hit
AGTGGAACTGTTATTATCCCTCAAATGGCACAGCTGAGACCAAGTCTCTG138021.2675095301986303No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCAAC130471.1981739487394238No Hit
TTAAATTTCACTCCACTTACCTACCAGTCAAATGTCCATGGATCATAGAC129221.186694547835582No Hit
TGGGGAAAAATATGCTTTGATCTACAGAAAAACATAGAAATCAGAGACCA127701.17273559633651No Hit
CACCTCGAACACCCAGGCCACTGACGACATGTTTCTTCTATAACCCACAC116431.0692373177874694No Hit
TGGGGAAAAATATGCTTTGAGCTACAGAAAAACATAGAAATCAGAGACCA113281.0403092275097874No Hit
AGTGGAACTGTTATTATCCCATCAAATGGCACAGCTGAGACCAAGTCTCT112261.0309420363722521No Hit
AGCACCAAACGTAAAGCAGCAACAGAGGAGGGGGAGACCAAGTCTCTGCT110331.0132178413767199No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG109581.0063302008344146No Hit
TCAAAGGGCTCCACTGGTTTTTCTTATCTCTTTCATTTGATTAGACCAAG108450.9959528224173415No Hit
CCCCTATTGCATATTTCTTCATGTGACACTGACGACATGTTTCTTCTATA107790.9898916987401128No Hit
GTAGACAACATAAAGCCTCATAATTACTCTACACTGACACAATGGCAACA104840.9628003126070453No Hit
GAAAGTGGTGCATTGATGAAGGCCTGATGTAGGTCTCAGACCAAGTCTCT103920.9543514735418176No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA98790.9072400122324495No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC98430.9039339447721432No Hit
TGAACTTATATATTTGACACAACCCAGCAACCCATTCAGCAGAATTAGAC96550.8866689258127645No Hit
CCTCTCTCCTTCTTCCCCAAGTACACTGACGACAGGGGCCAGGAGGAGAC96110.8826281766946121No Hit
GAAAGTGGTGCATTGATGGAAGGCCTGATGTAGGTCTCAGACCAAGTCTC95030.8727099743136926No Hit
TGTAGTTTCTAAGGGTCGAATTCTTTAAGTTCACTGGTATTTGAGACCAA93910.86242443110385No Hit
GCTTTCACTTCTCTGGCTGAAATGTGAACCAATATTAGCAAGACCAAGTC91890.8438737192432412No Hit
ATTATCCAGATGAATTTACAAAACTGTACATGTGCTCTTGAGTGAGACCA91660.8417615094769342No Hit
TCTAAGCATAGCATTCAATTTTGGCCACTACAAATCATTCTCCTTAAGAC87980.807966153216023No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTGTACCCTTTCGGTAAG86640.7956602354471043No Hit
GCTTTCACTTCTCTGACTGAAATGTGAACCAATATTAGCAAGACCAAGTC78540.7212737175902074No Hit
TCAAAGGGCTCCACAGTTTTTCTTATCTCTTTCATTTGATTAGACCAAGT77720.7137432305972871No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTACATACCT75600.6942741666643708No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT75080.6894987358883725No Hit
GGTGCCAGTTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACCG73990.6794886983002222No Hit
GGGCAGTTGGCCACTTTTACTTATGGCTGGGTGCAGAGACCAAGTCTCTG73530.6752642787676083No Hit
CACATCCTCCTAAACACACAGGAAGAGAGAGGGACAGGGAGACCAAGTCT73530.6752642787676083No Hit
GGGGTCAGAGGCAAGCAGATATCCGGTGTAGACCAAGTCTCTGCTACCGT72250.663509372242074No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC71830.659652293538383No Hit
GGCTCCATAGGAAAGTAACAGCAACAGAGGAGGGGGAGACCAAGTCTCTG70130.6440403083091577No Hit
GACAAAAATCATCTCTCCGAAAAACAAGTCTCTGCTACCGTAGCACACAG68170.6260406076919333No Hit
AGAGGAACGTTACACACCAGCAAATGATCAGCCTTGCAAAGACCAAGTCT67900.6235610570967034No Hit
GGGCAGTTGGCCACTTTACTTATGGCTGGGTGCAGAGACCAAGTCTCTGC67720.6219080233665502No Hit
AGAAATGTATAACCAAGTCAGTAAGGCACACTGACACAATGGCAACAGTT65670.6030818058842491No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC64690.5940819555756369No Hit
AGTTCCATTTAGGTTGCTGGTGAAGAACATTCAGGCAAGACCAAGTCTCT64650.5937146147467139No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA62840.5770924422379505No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTACATA58350.5358584341913497No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA53860.49462442614474883No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTACA52770.4846143885565985No Hit
ATTTACCCTACCAATTGCCCAGTACACTGACGACATGGTATTCGATGACT50010.4592678713609151No Hit
GCCTGCTGAAAATGCCAGAACCCCTCAGATGTTAGACCAAGTCTCTGCTA49570.4552271222427627No Hit
TCTACCCACTCTCTTTTCAGTGCTACGGTAGCAGAACTGTCAAATGACCA46640.4283194065241568No Hit
GCCTGCTGAAAATGACTGAACCCCTCAGATGTTAGACCAAGTCTCTGCTA45080.4139931141961618No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAC44610.4096768594563172No Hit
GGGGTCAGAGGAAGCAGATATCCGGTGTAGACCAAGTCTCTGCTACCGTA43920.4033402301573964No Hit
TGAATGTAGCACGCATTCACATAGGTTAACATTTACCACCCCTAGACCAA43300.39764644730909066No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTACATACC42200.3875445745137096No Hit
AGCACCAAACGTAAAGCAGAAAGTCAGTCCCATGGAGACCAAGTCTCTGC40990.37643251443879044No Hit
GTGCTGGTAAATTCACTCCATCGAATCATCACCAATAGACCAAGTCTCTG40740.374136634258022No Hit
TCTCCCCCACCCCTTCTACTCACTCAAAGACATCTGCAGACCAAGTCTCT39110.3591674954794119No Hit
CCAAATCTGCTTTCTTGATAAAATCCTCTGAATGGCATGCAGACCAAGTC38890.3571471209203357No Hit
TCTGGGGTATCAGGTAGGTGTCGTCAGTGTGGCTCTAATCAATCGACTCC37000.3397902667537265No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTACAT36960.3394229259248035No Hit
CATCTCCATGAGTAGGAAGAAGGCCTGATGTAGGTCTCAGACCAAGTCTC36950.3393310907175727No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACAAGGTA36540.33556584722111255No Hit
AGTTCCATTTAGGTTGCTGGTGGAGAACATTCAGGCAAGACCAAGTCTCT36060.3311577572740372No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCAAAAT34670.3183926634689647No Hit
TGTAGTTTCTAAGGGACAAATTCTTTAAGTTCACTGGTATTTGAGACCAA33230.3051683936277386No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTACATACCTGATATC30500.28009738205374746No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTACATAC29700.2727505654752885No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGAAAAATGAAATAC29190.26806696990652096No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTACATA28850.2649445728606759No Hit
TCTCCCCCACCCCCTCTACTCACTCAAAGACATCTGCAGACCAAGTCTCT28360.26044464770636977No Hit
CTACTAATGCCCACAACGAGATACTTTCCTGAGTGCCAGACCAAGTCTCT28330.26016914208467756No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACAAAATGAAATA27490.2524549846772957No Hit
GTGGACAAGTTTGACATTATTGGGATACACTGACGACATGGTTCTACAGA26510.24345513436868346No Hit
GATAAATTCAGTTTTTATTCTCAGTTATTCAGTACACTGACGACATGGTT25880.23766951631314703No Hit
GTGGACAAGTTTGACATTATTGGGATACACTGACGACATGGTTCTACATA24830.22802681955391965No Hit
CTACTAATGCCCACAAAGAGATACTTTCCTGAGTGCCAGACCAAGTCTCT24740.227200302688843No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT23740.21801678196576935No Hit
TGGCTTATAAAATATTAATGTGCTTCTGTTTCAAAAGACCAAGTCTCTGC22900.21030262455838744No Hit
TGGTGATTTGCTGATATACCATGTCTCTGCTACCGTACCCTATGTATGCT22230.2041496656739281No Hit
CATCTCCATGAGTAGGATGAAGGCCTGATGTAGGTCTCAGACCAAGTCTC22040.2024047967365441No Hit
ATTATCCAGATGAATTTACAAAACTGTACCTGTGCTCTTGAGTGAGACCA20900.19193558311224007No Hit
TGCATTCTGAAAAAGCCAGAATTTACAGCTCTCCAGACCAAGTCTCTGCT20800.1910172310399327No Hit
GGGATAAAAGCACAAAGGTCTGCTACCGTACCTTCAGAGCTAGGGAAAAA19980.1834867440470123No Hit
AATCTCCAAGGAAGTTGTACCGTCTTAGACCAAGTCTCTGCTACCGTACA19480.17889498368547543No Hit
CACTTAATGAGACCAACAGTAACACCTTTATCACTCACAAGACCAAGTCT18680.17154816710701648No Hit
ACAGAGTTTGAATGGATATTGTGGGTACACTGACGACATGGCTCTACAAC18310.16815026443947922No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTACATAC18020.16548704342978787No Hit
GGGATAAAAGCACAAAGGTCTGCTACCGTAACCTTCAGAGCTAGGGAAAA17800.16346666887071165No Hit
GTAACTTGATTTGCTGTTGTATTTGCTACCGTAACCCTCAATTTGGAAGC17670.16227281117671208No Hit
GCCACAAAGTAAAAATGTTGTGTGTTTACACTGACGACAGGTTTCTTAGA16210.14886487092102446No Hit
GCTTTCACTTCTCTGGCTGAACACACAGCTCAGAATACTAGTAGACCAAG16150.14831385967764005No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTACATACC15610.14335475848718027No Hit
CTGGTGTTGTTGGGCAGTGCAGCTCAGGTTGCAAAACCCCTAATCTAGAC15350.1409670430991811No Hit
AAAAGCAGTGGTCCAATGTATCTGCTACCGTAGAGCAGTCAGAGGACCAG15250.14004869102687373No Hit
TGAATTCTCCTCAGATGACTCCATTTAAAGACCAAGTCTCTGCTACCGTA15170.13931400936902782No Hit
TATGGTGAAGAACATTCAGGCAAGACCAAGTCTCTGCTACCGTACATACC15150.13913033895456636No Hit
AGCTGCTCACCATCGCTACCGTCAGCAAATGATCAGCCTTGCAAAGACCA14970.1374773052244131No Hit
TTGGACAAGTTTGACATTATTGGGATACACTGACGACATGGTTCTACATA14890.1367426235665672No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG14880.13665078835933647No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG14310.13141618154718446No Hit
GACTACAGATGTGCCACCATACCAACTGCTCTGGAACATGTTAGACCAAG14310.13141618154718446No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAAAGACCAAGTC13770.12645708035672468No Hit
CCAGACTCTCCAGAACCAAAGGTGCTGACTACTACCGCTAGACCAAGTCT12990.1192939341927272No Hit
ATTTACCCTACCAATTGCCCAGTACACTGACGACATGGTTCTACAATTTA12980.11920209898549647No Hit
GGATGAGAACTCCGAGAAGCAGCATATAAAAATGACTCTAGGTAGACCAA12980.11920209898549647No Hit
GAGATTGATGACCAAAAGAACTGCACACTGACGACATGGTTCTCCTCAGA12780.11736539484088174No Hit
CCCAAGATTACTGGCATCTTCCTACACTGACCCATGTGGAATAGTTCCCA12630.11598786673242067No Hit
ATTTAACACCTAGCCAAAAGGCAGACCAAGTCTCTGCTACCGTACATACC12400.11387565696611374No Hit
TCCACAAACAGAACAAGATGCTTACGGTAGCAGAAGCCTTTTTAGCCCTA12240.11240629365042193No Hit
GCCACACCTCACGTTAGAATTTTCCAGTGAAGACCAAGTCTCTGCTACCG12230.11231445844319118No Hit
GCCCTCTCAGAAACATCTGCTACCGTAACTTCGAGGGCTAAACCACAAGA12170.11176344719980677No Hit
AGTAAGGGTGGCATCAAAAATTGGTTTTCTTTGCAGACCAAGTCTCTGCT12040.1105695895058072No Hit
GTTTATTTGAGGGGAAGCCAAATCACAGTTATTTCTTAAGAGACCAAGTC11720.10763086287442361No Hit
GTGGACAAGTTTGACATTATTGGGATACACTGACGACATGGTTCTACAGT11650.10698801642380845No Hit
GCGTCTTCACCTGGAAGGCAGTCTGGCTGAGACCAAGTCTCTGCTACCGT11570.10625333476596258No Hit
GCTGCCCCAAAGTGTATAGAAGCCAAAATGATAAGAAACTAAGAGACCAA11500.10561048831534742No Hit
TCATGCTGTTTACATTCACTAAGGCTACGGTAGCAGAGTCACAGTCACAG11450.10515131227919372No Hit
TCTATTGTGATGGCCACGTTACACTGACGACATGGTAAAGAGACTTCAGT11150.10239625606227162No Hit
AATATGTGCTTCTTACCAGTCAAAAAGTATACACTGACGACATGGTGGTT11060.10156973919719497No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)