FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1088907
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG220582.025701001095594No Hit
TGCTCACTTGATACTTAGTTTGCTTTTCGGTGTTCTGAAACAACTGTAGA193431.7763684134641435No Hit
ATGTGTCCCAGCTAAATGTGGTGTCCTTTGCATTCAGTATGTAGAACCAT185931.7074920080410907No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA184521.6945432438215569No Hit
CGACTCAGAGACCGCTTGCTGGGAATTGTAGTTAATGTAGAACCATGTCG177111.6264933552635807No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC164271.5085769491793146No Hit
GCATGCCATTCAAAGGATTTTATCAAGAAAGCAGATTTGGTGTAGAACCA162941.4963628666176267No Hit
TCTGGATCAGAGTCAGTGGTGCCTTTCTTCATCCATATTACTTTATACTC160311.4722102071159429No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT155451.4275782964018047No Hit
TACTGGTGTGGGTTATAGAAGAAACATGTCGTCAGTGTACAGCAAACAGG154971.4231702064547294No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT153871.4130683336593484No Hit
ACTTTCAAGTTTCCCCACCCTTTCTCTGAGGCCCTGAGGTGTAGAACCAT148801.3665078835933648No Hit
CAGAACTGTTGCCATTGTGTCAGTGTGGCAAAGTTGAAGTACTGGAAGCT145901.339875673496451No Hit
GGTTGAACTAAAACGACAATACAATGAAAGGTTTGTACCGGTGTAGAACC145371.335008407513222No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTACTACTGTCTTTTC140831.2933152234304675No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTACAGATAATACAAG137921.266591178126323No Hit
CAGCTGTGCCATTTGAGGGATAATAACAGTTCCACTTGTAGAACCATGTC136921.2574076574032493No Hit
ATGATCCATGGACATTTGACTGGTAGGTAAGTGGAGTGAAATTTAATGTA126921.1655724501725124No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG126221.1591439856663608No Hit
CTGATTTCTATGTTTTTCTGTAGATCAAAGCATATTTTTCCCCATGTAGA124851.1465625622757498No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTGGGGAAGAAGGAGAGAGGTGTA120511.1067060823376103No Hit
TACTGGTGTGGGTTATAGAAGAAACATGTCGTCAGTGGCCTGGGTGTTCG113291.040401062717018No Hit
CAGCTGTGCCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGT111211.0212993396130248No Hit
CTGATTTCTATGTTTTTCTGTAGCTCAAAGCATATTTTTCCCCATGTAGA111141.0206564931624096No Hit
CCCCCTCCTCTGTTGCTGCTTTACGTTTGGTGCTTGTAGAACCATGTCGT109941.0096362682947213No Hit
AATCAAATGAAAGAGATAAGAAAAACCAGTGGAGCCCTTTGATGTAGAAC106950.9821775413327309No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG105490.9687696010770432No Hit
TACTGGTGTGGGTTATAGAAGAAACATGTCGTCAGTGTCACATGAAGAAA103270.9483821850718197No Hit
CAGAACTGTTGCCATTGTGTCAGTGTAGAGTAATTATGAGGCTTTATGTT103140.9471883273778202No Hit
GAGACCTACATCAGGCCTTCATCAATGCACCACTTTCTGTAGAACCATGT102980.9457189640621283No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT97840.8985156675455295No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG97600.8963116225719918No Hit
AATTCTGCTGAATGGGTTGCTGGGTTGTGTCAAATATATAAGTTCATGTA94310.8660978393930794No Hit
GAGACCTACATCAGGCCTTCCATCAATGCACCACTTTCTGTAGAACCATG93580.8593938692652356No Hit
CAAATACCAGTGAACTTAAAGAATTCGACCCTTAGAAACTACATGTAGAA92630.8506695245783158No Hit
TGCTAATATTGGTTCACATTTCAGCCAGAGAAGTGAAAGCTGTAGAACCA90500.8311086254381688No Hit
CACTCAAGAGCACATGTACAGTTTTGTAAATTCATCTGGATAATTGTAGA90480.8309249550237072No Hit
TAAGGAGAATGATTTGTAGTGGCCAAAATTGAATGCTATGCTTAGATGTA86280.7923541679867977No Hit
ACATGTCTTACCGAAAGGGTACACTTGGTCTGTTGAAATTGAGAGAGATA84390.7749973138201885No Hit
TGCCTGAATGTTCTTCACCAGCAACCTAAATGGAACTTGTAGAACCATGT77780.7142942418406714No Hit
TGCTAATATTGGTTCACATTTCAGTCAGAGAAGTGAAAGCTGTAGAACCA77420.7109881743803649No Hit
AATCAAATGAAAGAGATAAGAAAAACTGTGGAGCCCTTTGATGTAGAACC76870.7059372379826744No Hit
TGCCTGAATGTTCTTCACCATATGTAGAACCATGTCGTCAGTGTATCCCA75290.691427275240218No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC75100.689682406302834No Hit
GAGTCCTCCTTCTGTGAGCAAACTGGCACCTGTAGAACCATGTCGTCAGT73970.6793050278857606No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT73910.6787540166423762No Hit
CTGCACCCAGCCATAAGTAAAAGTGGCCAACTGCCCTGTAGAACCATGTC72810.6686521438469952No Hit
CCCTGTCCCTCTCTCTTCCTGTGTGTTTAGGAGGATGTGTGTAGAACCAT72580.6665399340806882No Hit
ACACCGGATATCTGCTTGCCTCTGACCCCTGTAGAACCATGTCGTCAGTG71850.6598359639528445No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG71300.6547850275551539No Hit
CCCCCTCCTCTGTTGCTGTTACTTTCCTATGGAGCCTGTAGAACCATGTC69270.6361424804873143No Hit
CAGTCAAGAGTCATCGAATACCATGTCGTCAGTGTACTGGGCAATTGGTA67110.6163060757254751No Hit
TTGCAAGGCTGATCATTTGCTGGTGTGTAACGTTCCTCTTGTAGAACCAT66910.6144693715808605No Hit
CTGCACCCAGCCATAAGTAAAGTGGCCAACTGCCCTGTAGAACCATGTCG66660.612173491400092No Hit
ACTAGTATTCTGAGCTGTGTGCTACGGTAGCAGAGACTTGTTTTTCGGAG66410.6098776112193236No Hit
CAGAACTGTTGCCATTGTGTCAGTGTGCCTTACTGACTTGGTTATACATT64320.5906840529080996No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT62350.5725925170836444No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT58040.5330115427671968No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG53880.4948080965592103No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT52840.4852572350072136No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG51930.4769002311492166No Hit
AACATCTGAGGGGTTCTGGCATTTTCAGCAGGCTGTAGAACCATGTCGTC49140.451278208331841No Hit
TGGTCATTTGACAGTTCTGCTACCGTAGCACTGAAAAGAGAGTGGGTAGA45400.4169318408275454No Hit
AACATCTGAGGGGTTCAGTCATTTTCAGCAGGCTGTAGAACCATGTCGTC44730.41077888194308604No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA44190.40581978075262626No Hit
TGCACTGGTAAAGAAAACTGAGAATAAAAACTGAGAATAAAAACTGAATT43960.40370757098631926No Hit
ACACCGGATATCTGCTTCCTCTGACCCCTGTAGAACCATGTCGTCAGTGT43210.39681993044401404No Hit
AGGGGTGGTAAATGTTAACCTATGTGAATGCGTGCTACATTCATGTAGAA42680.391952664460785No Hit
GGAGAGCTGTAAATTCTGGCTTTTTCAGAATGCATGTAGAACCATGTCGT42440.3897486194872473No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT41770.38359566060278794No Hit
CCATGGGACTGACTTTCTGCTTTACGTTTGGTGCTTGTAGAACCATGTCG40510.3720244244917151No Hit
ATTGGTGATGATTCGATGGAGTGAATTTACCAGCACTGTAGAACCATGTC39670.3643102670843332No Hit
GCAGATGTCTTTGAGTGAGTAGAAGGGGTGGGGGAGATGTAGAACCATGT38990.3580654729926431No Hit
GCATGCCATTCAGAGGATTTTATCAAGAAAGCAGATTTGGTGTAGAACCA38450.3531063718021833No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA37020.3399739371681879No Hit
GAGACCTACATCAGGCCTTCTTCCTACTCATGGAGATGTGTAGAACCATG36560.33574951763557404No Hit
CCTGGAGTCGATTGATTAGAGCCACACTGACGACACCTACCTGATACCCC36140.3318924389318831No Hit
TGCCTGAATGTTCTCCACCAGCAACCTAAATGGAACTTGTAGAACCATGT36050.3310659220668064No Hit
TGCACGTACCACATTTAATTACCTTGTAGCAGAGACTTGGTCTTCTCTTG35450.3255558096329622No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTGACTTGGTCTGTCTAAGAACACA34380.31572944245927337No Hit
CAAATACCAGTGAACTTAAAGAATTTGTCCCTTAGAAACTACATGTAGAA33140.304341876762662No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT31710.2912094421286666No Hit
GCAGATGTCTTTGAGTGAGTAGAGGGGGTGGGGGAGATGTAGAACCATGT31460.2889135619478982No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC29450.2704546852945201No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTTCTGTCTAAGAACACAGAGGAGA29110.26733228824867505No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT28470.2614548349859079No Hit
GGCACTCAGGAAAGTATCTCGTTGTGGGCATTAGTAGTGTAGAACCATGT28430.26108749415698496No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTGTCTGTCTAAGAACACAGAGGAG27050.24841423555914324No Hit
GGCACTCAGGAAAGTATCTCTTTGTGGGCATTAGTAGTGTAGAACCATGT24800.22775131393222747No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT23390.21480254971269355No Hit
TTTGAAACAGAAGCACATTAATATTTTATAAGCCATGTAGAACCATGTCG22550.20708839230531165No Hit
GAGACCTACATCAGGCCTTCATCCTACTCATGGAGATGTGTAGAACCATG21890.20102726862808304No Hit
ACACAACAAAGAGCATACATAGGGTACGGTAGCAGAGACATGGTATATCA21020.19303760559900893No Hit
CACTCAAGAGCACAGGTACAGTTTTGTAAATTCATCTGGATAATTGTAGA20650.18963970293147164No Hit
TTAAATGGAGTCATCTGAGGAGAACCATGTCGTCAGTGTGCAGTTCTTTT20430.18761932837239545No Hit
AAGACGGTACAACTTCCTTGGAGATTTGTAGAACCATGTCGTCAGTGTAG19160.17595625705409187No Hit
ACTTTTTCCCTAGCTCTGAAGGTACGGTAGCAGACCTTTGTGCTTTTATC18350.16851760526840218No Hit
TGTGAGTGATAAAGGTGTTACTGTTGGTCTCATTAAGTGTGTAGAACCAT18230.16741558278163335No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC17800.16346666887071165No Hit
TGCTAAGAAACAGGCATCCATTCAAACTCTGTTGTAGAGCCATGTCGTCA17190.15786472122963668No Hit
AAGAGGCTTCCAAATTGAGGGTTACGGTAGCAAATACAACAGCAAATCAA17060.1566708635356371No Hit
GCAAAGAAAACCAATTTTTGATGCCACCCTTACTTGTAGAACCATGTCGT16900.15520150021994533No Hit
ACTTTTTCCCTAGCTCTGAAGGTTACGGTAGCAGACCTTTGTGCTTTTAT16710.15345663128256132No Hit
CTTAAGAAATAACTGTGATTTGGCTTCCCCTCAAATAAACTGTAGAACCA16560.15207910317410028No Hit
CAGGACTAGCTCTAAGAAACCTGTCGTCAGTGTAAACACACAACATTTTT16030.14721183719087122No Hit
ACTAGTATTCTGAGCTGTGTGTTCAGCCAGAGAAGTGAAAGCTGTAGAAC15970.14666082594748678No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT15440.14179355996425774No Hit
CCTGGTCCTCTGACTGCTCTACGGTAGCAGATACATTGGACCACTGCTTT15130.13894666854010487No Hit
AGATTAGGGGTTTTGCAACCTGAGCTGCACTGCCCAACAACACCAGTGTA15080.1384874925039512No Hit
TGCCTGAATGTTCTTCACCATATGTAGAACCATGTCGTCAGTGTAGATCT15010.13784464605333605No Hit
TTAAATGGAGTCATCTGAGGAGAATTCATGTAGAACCATGTCGTCAGTGT14920.1370181291882594No Hit
TTGCAAGGCTGATCATTTGCTGACGGTAGCGATGGTGAGCAGCTTGTAGA14650.1345385785930295No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT14630.13435490817856804No Hit
AACATGTTCCAGAGCAGTTGGTATGGTGGCACATCTGTAGTCTGTAGAAC14090.12939580698810826No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT13940.1280182788796472No Hit
TTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA13580.12471221141934068No Hit
AGCGGTAGTAGTCAGCACCTTTGGTTCTGGAGAGTCTGGTGTAGAACCAT12950.11892659336380426No Hit
ACCTAGAGTCATTTTTATATGCTGCTTCTCGGAGTTCTCATCCTGTAGAA12700.11663071318303583No Hit
GGGAACTATTCCACATGGGTCAGTGTAGGAAGATGCCAGTAATCTTGGGT12400.11387565696611374No Hit
GCCTTTTGGCTAGGTGTTAAATTGTAGAACCATGTCGTCAGTGTAGATCT12290.11286546968657563No Hit
TGTGGTTTAGCCCTCGAAGTTACGGTAGCAGATGTTTCTGAGAGGGCTGT12030.11047775429857645No Hit
TCACTGGAAAATTCTAACGTGAGGTGTGGCTGTAGAACCATGTCGTCAGT11960.1098349078479613No Hit
AGGGCTAAAAAGGCTTCTGCTACCGTAAGCATCTTGTTCTGTTTGTGGAT11930.10955940222626909No Hit
CAGCCAGACTGCCTTCCAGGTGAAGACGCTGTAGAACCATGTCGTCAGTG11510.10570232352257813No Hit
ATGTTACCTTAAGATCAACCACCATGTCGTCAGTGTATACTTTTTGACTG11250.10331460813457899No Hit
CTTAGTTTCTTATCATTTTGGCTTCTATACACTTTGGGGCAGCTGTAGAA11210.10294726730565604No Hit
AACCTACCTGTGACTGTGACTCTGCTACCGTAGCCTTAGTGAATGTAAAC11080.10175340961165646No Hit
GGAAGTTTAACTGAAGTCTCTTTACCATGTCGTCAGTGTAACGTGGCCAT11010.1011105631610413No Hit
CGCATGGATACAGAAATGGAGGACTTTGTAGAACCATGTCGTCAGTGTAG10940.10046771671042615No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)