FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences789652
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACGCTCTTGTATTATCTGTACACTGACTTATGGTATCAAATAATAA219602.7809718711533686No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC207342.625713605486974No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC185202.3453369332313474No Hit
TACTGAATGCAAAGGACACCACATTTAGCTGGGACACATAGACCAAGTCT173962.2029957500265938No Hit
CCAAATCTGCTTTCTTGATAAAATCCTTTGAATGGCATGCAGACCAAGTC169012.140309908668629No Hit
TTAACTACAATTCCCAGCAAGCGGTCTCTGAGTCGAGACCAAGTCTCTGC168002.127519464270337No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT157861.99910846803402No Hit
GACTACAGATGTGCCACCATACCAACTGCTCTGGAACATGTTAGACCAAG142681.8068718878695933No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAAAGACCAAGTC131791.6689630368820696No Hit
AGTGGAACTGTTATTATCCCTCAAATGGCACAGCTGAGACCAAGTCTCTG131621.6668101898051295No Hit
TGGGGAAAAATATGCTTTGATCTACAGAAAAACATAGAAATCAGAGACCA123131.5592944740214676No Hit
TCTAAGCATAGCATTCAATTTTGGCCACTACAAATCATTCTCCTTAAGAC120211.5223161595234356No Hit
ATTATCCAGATGAATTTACAAAACTGTACATGTGCTCTTGAGTGAGACCA112441.4239183843009326No Hit
AGTGGAACTGTTATTATCCCATCAAATGGCACAGCTGAGACCAAGTCTCT107981.367437808047089No Hit
TGAATGTAGCACGCATTCACATAGGTTAACATTTACCACCCCTAGACCAA107961.3671845319203904No Hit
GTGGTGGTATACGATATGGGTTCTACATGAAAAAGAGCAAGGTACTAGTG104541.323874314254887No Hit
AGAGGAACGTTACACACCAGCAAATGATCAGCCTTGCAAAGACCAAGTCT101951.291075055847386No Hit
TGGGGAAAAATATGCTTTGAGCTACAGAAAAACATAGAAATCAGAGACCA101911.2905685035939882No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC93361.18229295943023No Hit
GAGATTGATGACCAAAAGAACTGCTACCGTATTCTCCTCAGATGACTCCA93001.1777339891496508No Hit
TCAAAGGGCTCCACTGGTTTTTCTTATCTCTTTCATTTGATTAGACCAAG90051.1403757604615703No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA88561.121506689022506No Hit
TTGGACTCAATTTCAACTCTAACTTTAAATGCAAACTAGGCCAGGCAGAC81861.0366591865783914No Hit
GCTGCCCCAAAGTGTATAGAAGCCAAAATGATAAGAAACTAAGAGACCAA79981.0128512306686996No Hit
GAAAGTGGTGCATTGATGGAAGGCCTGATGTAGGTCTCAGACCAAGTCTC79911.0119647642252536No Hit
CTTAAATGTTGAAGGTGGTTCTTCAGCAAATTGGAAAGGTAAAGTAAAGA79551.0074057939446743No Hit
TGTAGTTTCTAAGGGTCGAATTCTTTAAGTTCACTGGTATTTGAGACCAA77730.9843576664150789No Hit
GAAAGTGGTGCATTGATGAAGGCCTGATGTAGGTCTCAGACCAAGTCTCT76980.9748598116638721No Hit
TGAACTTATATATTTGACACAACCCAGCAACCCATTCAGCAGAATTAGAC75620.9576370350483504No Hit
GCTGCCCCAAAGTGTAAAGAAGCCAAAATGATAAGAAACTAAGAGACCAA72890.9230648437539575No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCAAC71060.8998900781610126No Hit
AGCACCAAACGTAAAGCAGCAACAGAGGAGGGGGAGACCAAGTCTCTGCT67640.8565798604955094No Hit
GTTGTTTCAGAACACCGAAAGCTCAAGAAGCATGTCATAGACCAAGTCTC66680.8444226064139646No Hit
GGGCAGTTGGCCACTTTTACTTATGGCTGGGTGCAGAGACCAAGTCTCTG66010.8359378561695531No Hit
TTAGCATGCCAATGTTTAAAATGTAAATGATTATTTAAAGGCAAAAGAAA64250.8136495570200544No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA63450.8035185119521004No Hit
GATTCCCGCGCCCAGAGTCCCACGACCCCCTCAGACCAAGTCTCTGCTAC60280.7633742458703328No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTATC60220.7626144174902362No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTATCTGGTC59800.7572956188295603No Hit
TGGGTCCAGTCAGTGAACGAGATACTTTCCTGAGTGCCAGACCAAGTCTC58870.7455182789380639No Hit
CCGGTACAAACCTTTCATTGTATTGTCGTTTTAGTTCAACCAGACCAAGT57110.7232299797885651No Hit
TTCTGATGAGCCAAGTCTCTGTGGGAGGAGGGCAGTACACAGACCAAGTC56960.7213304088383238No Hit
AGTTCCATTTAGGTTGCTGGTGGTGAAGAACATTCAGGCAAGACCAAGTC56590.716644800494395No Hit
AGTTCCATTTAGGTTGCTGGTGAAGAACATTCAGGCAAGACCAAGTCTCT56110.7105661734536226No Hit
GGGCAGTTGGCCACTTTACTTATGGCTGGGTGCAGAGACCAAGTCTCTGC55910.7080334121866342No Hit
GTTGTTTCAGAACACCGAAAAGCTCAAGAAGCATGTCATAGACCAAGTCT55580.703854356096103No Hit
TCAAAGGGCTCCACAGTTTTTCTTATCTCTTTCATTTGATTAGACCAAGT51750.6553519778332734No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTATCT51520.6524393023762366No Hit
TTTTAAGATGGTGTTTAAAAGAAGTAACCCTCAATTTGGAAGCCTCTTAG45570.5770896546833288No Hit
GGTGCCAGTTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACCG44230.5601201541945059No Hit
AGACATCGCTGAATTTAAAGGATACAGATGAAATTGCTGCCACAGACCAA44150.5591070496877105No Hit
CATCTCCATGAGTAGGAAGAAGGCCTGATGTAGGTCTCAGACCAAGTCTC43750.5540415271537336No Hit
GGGGTCAGAGGCAAGCAGATATCCGGTGTAGACCAAGTCTCTGCTACCGT43570.5517620420134439No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC43030.544923586592575No Hit
GCTTTCACTTCTCTGGCTGAACACACAGCTCAGAATACTAGTAGACCAAG42490.538085131171706No Hit
TCTCCCCCACCCCCTCTACTCACTCAAAGACATCTGCAGACCAAGTCTCT41180.5214955448729314No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT41040.5197226119860394No Hit
TTACTGCAGCTCACAAATGCTTTTTTACATCTGCAAGAAATTAACTAGTC40970.5188361455425935No Hit
AGTCTTCTCCTAAACCATAGTCTGCCTTTTGATAGCTCTAATAATTAATT39590.5013600928003729No Hit
CCAAAATATGTCTGGATTGGAGAGAAATCTGTATTAACAGTCTGAGACCA39580.5012334547370234No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTATCTG38900.49262206642926254No Hit
AAAAGCAGTGGTCCAATGTATCTGCTACCTTAATCTTTGTCAACACTACC37120.4700804911530649No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTAAAATG36600.4634953118588948No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTAAAATGAAATACTTTGAGAA36590.46336867379554536No Hit
CCAGTGCAACAGGTGGCTTGGGATCAATAAAAGCTGAGGTGATGGCAGAT36460.4617223789720028No Hit
CCAGAGACCAGACTCCAGAGCAAGTCTCTGCTACCGTACATCAATCATTC35750.4527310764741937No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA35620.4510847816506512No Hit
TTAAATTTCACTCCACTTACCTACCAGTCAAATGTCCATGGATCATAGAC33930.4296829489445984No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC32370.40992741106208813No Hit
TGGCTTATAAAATATTAATGTGCTTCTGTTTCAAAAGACCAAGTCTCTGC31700.40144266081767666No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTATCTGGT31610.4003029182475319No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC30770.3896653209261801No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACAAAATGAAATA29540.37408883913420093No Hit
CATCTCCATGAGTAGGATGAAGGCCTGATGTAGGTCTCAGACCAAGTCTC29310.3711761636771641No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTA25870.327612669884962No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA25700.32545982280802177No Hit
AGTTCCATTTAGGTTGCTGGTGGAGAACATTCAGGCAAGACCAAGTCTCT25150.3184947293238034No Hit
CAAAGTGGGAAGGGGAGTGAACATCGAATCATGGAATCCCAGACCAAGTC24980.31634188224686316No Hit
CAAAGTGGGAAGGGGAGTGAATATCGAATCATGGAATCCCAGACCAAGTC24070.3048178184820655No Hit
GCCTGCTGAAAATGCCAGAACCCCTCAGATGTTAGACCAAGTCTCTGCTA23900.30266497140512527No Hit
CTACAGTTATTTATTACCCCAGAAGCAAAGAAATAATACCCATGACAGGA23520.29785272499784715No Hit
GTCTCAGAACAAACCTGAGATGCGAACCATGGTCAGGTTGCAAAACCCCT22440.2841758141561093No Hit
AACTGATCACCTTGAATCTGCTTGGAGAAGGAATATTTGATGGTCAACCA22140.2803766722556265No Hit
CACTTAATGAGACCAACAGTAACACCTTTATCACTCACAAGACCAAGTCT21860.27683080648184266No Hit
TCTCCCCCACCCCTTCTACTCACTCAAAGACATCTGCAGACCAAGTCTCT21410.2711320936311185No Hit
AGAAGACCAGGAAAGAACGGGAGCTTCTTATGGCCAAGAGACCAAGTCTC20880.264420276273599No Hit
GCCTGCTGAAAATGACTGAACCCCTCAGATGTTAGACCAAGTCTCTGCTA20350.25770845891607946No Hit
CAGCAATGTGGCTTTAAGTAGAACATATCATCTGGACCCTTTTCAGACCA20000.25327612669884964No Hit
CCAAATCTGCTTTCTTGATAAAATCCTCTGAATGGCATGCAGACCAAGTC19610.24833724222822207No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTATCTGGTCTCAATC19390.24555120483453471No Hit
CTACTAATGCCCACAAAGAGATACTTTCCTGAGTGCCAGACCAAGTCTCT19040.24111887261730483No Hit
TTTTAAGATGGTGTTTAAAAGAAGTAACCCAGAACTGTCAAATGACCAAG18880.23909266360371403No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTATCTG18440.23352058881633936No Hit
ATTATCCAGATGAATTTACAAAACTGTACCTGTGCTCTTGAGTGAGACCA18140.22972144691585658No Hit
GGGGTCAGAGGAAGCAGATATCCGGTGTAGACCAAGTCTCTGCTACCGTA18060.2287083424090612No Hit
CTACTAATGCCCACAACGAGATACTTTCCTGAGTGCCAGACCAAGTCTCT17560.22237643924158998No Hit
GTGCTGGTAAATTCACTCCATCGAATCATCACCAATAGACCAAGTCTCTG16990.21515806963067277No Hit
TGCATTCTGAAAAAGCCAGAATTTACAGCTCTCCAGACCAAGTCTCTGCT16970.2149047935039739No Hit
TGTAGTTTCTAAGGGACAAATTCTTTAAGTTCACTGGTATTTGAGACCAA16790.21262530836368426No Hit
GAAGTATTACAAATGGAAGATGATCTGGTGCACTTTTCTGAGGACCATAA16410.20781306195640611No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTATCT16150.20452047230932108No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACAAGGTA15110.19135011372098087No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTATCTGG15010.19008373308748663No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAT14950.18932390470739008No Hit
TGCCAAAATGACGAACACAAAGGGAAAGAGGAGAGGCACCCGATACGTGT14750.1867911434404016No Hit
CACTTAATGAGACCAACAGTAACACCACTGACTCTGATCCAGAAGACCAA13940.17653346030909817No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCCAACACCAG13550.17159457583847063No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCCAACACCAA13460.17045483326832578No Hit
GCCTACCAAAATCAGAGAAGCCATGTCGCAGTGTACAGGTTTCTTAGAGC13110.16602250105109592No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCTCCAACACCAG12990.1645028442909028No Hit
GCCACACCTCACGTTAGAATTTTCCAGTGAAGACCAAGTCTCTGCTACCG12750.16146353077051664No Hit
GGCTTCCACTTAATTCCAGTCTAAAAAGTTACCCAGAAGTGTAGACCAAG12510.15842421725013042No Hit
CTTGAGGCATTGTTATTTCAGCATTCTTTTCTCTCACACAGGGGATCAGA12000.15196567601930977No Hit
GCTTTCACTTCTCTGGCTGAGCACACAGCTCAGAATACTAGTAGACCAAG11530.1460136870418868No Hit
TCCTCCCCTCTTAGCTGTCTTAGTGAAAGGTATGATGAAGCTAGACCAAG11160.1413280786979581No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCAAAAT10940.13854204130427075No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGAAAATGAAATACTTTGA10490.13284332845354663No Hit
TGAATGTAGCACGCATTCACATAAGTTAACATTTACCACCCCTAGACCAA10350.13107039556665467No Hit
TCCCTCTCAGAAACATCTGCACACTGACGACATGGTTCTACATTCCTGCC10000.12663806334942482No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAAATGAAAT9990.1265114252860754No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG9970.12625814915937653No Hit
GATGTGCCACCATACCCAGAGACCAAGTCTCTGCTACCGTATCTGGTCTC9430.11941969373850761No Hit
GCGTCTTCACCTGGAAGGCAGTCTGGCTGAGACCAAGTCTCTGCTACCGT9290.11764676085161566No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG9140.11574718990137428No Hit
GTTTGAATCCTGTTTGAAGGAAAAGGCCAGACCAAGTCTCTGCTACCGTA8940.11321442863438577No Hit
CACTTAATGAGACCAACAGTGACACCTTTATCACTCACAAGACCAAGTCT8670.10979520092395131No Hit
TCGGGGGCCTACAAGAGTCTGACAGAGTTCTCATTACCCAAGACCAAGTC8580.10865545835380648No Hit
GTGCACATTCAAAAAAGAAATTATCCAGATTTCCTTTCAGAACACTCAGA8380.10612269708681799No Hit
CTGAAAGCTGTACCATACCTGTCAGGAGACCAAGTCTCTGCTACCGTATC8290.10498295451667317No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT8190.10371657388317893No Hit
CTGGCTGAGCACACAGCTCAGAATACTAGTAGACCAAGTCTCTGCTACCG8140.1030833835664318No Hit
CAGCAATGTGGCTTTAATTAGAACATATCATCTGGACCCTTTTCAGACCA8060.1020702790596364No Hit
TGAAGAAGGAGATGGTGGGTAATTAATCAGATAAACATTTTAGTAGACCA7960.10080389842614214No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)