FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100590

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100590
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences789652
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTACAGATAATACAAG216452.7410808811983No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG204562.5905082238758337No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA182992.3173499212311244No Hit
ATGTGTCCCAGCTAAATGTGGTGTCCTTTGCATTCAGTATGTAGAACCAT170642.160951912994585No Hit
GCATGCCATTCAAAGGATTTTATCAAGAAAGCAGATTTGGTGTAGAACCA167392.119794542406022No Hit
CGACTCAGAGACCGCTTGCTGGGAATTGTAGTTAATGTAGAACCATGTCG166682.1108032399082126No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC155311.9668157618799167No Hit
AACATGTTCCAGAGCAGTTGGTATGGTGGCACATCTGTAGTCTGTAGAAC140521.7795180661861174No Hit
TTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA129891.644901804845679No Hit
CAGCTGTGCCATTTGAGGGATAATAACAGTTCCACTTGTAGAACCATGTC129591.6411026629451961No Hit
CTGATTTCTATGTTTTTCTGTAGATCAAAGCATATTTTTCCCCATGTAGA121111.533713585224884No Hit
TAAGGAGAATGATTTGTAGTGGCCAAAATTGAATGCTATGCTTAGATGTA118161.4963553565368037No Hit
CACTCAAGAGCACATGTACAGTTTTGTAAATTCATCTGGATAATTGTAGA111521.4122676824727853No Hit
CAGCTGTGCCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGT106901.3537608972053512No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG106711.3513547740017122No Hit
AGGGGTGGTAAATGTTAACCTATGTGAATGCGTGCTACATTCATGTAGAA106141.3441364043907948No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCCATATCGTATACCACCAC103591.3118436982366917No Hit
TTGCAAGGCTGATCATTTGCTGGTGTGTAACGTTCCTCTTGTAGAACCAT100531.2730924508517676No Hit
CTGATTTCTATGTTTTTCTGTAGCTCAAAGCATATTTTTCCCCATGTAGA99981.2661273573675493No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG92391.1700090672853358No Hit
TTAAATGGAGTCATCTGAGGAGAATACGGTAGCAGTTCTTTTGGTCATCA91561.1594981080273337No Hit
AATCAAATGAAAGAGATAAGAAAAACCAGTGGAGCCCTTTGATGTAGAAC90781.1496203390860784No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT87071.1026376175834418No Hit
GCCTGGCCTAGTTTGCATTTAAAGTTAGAGTTGAAATTGAGTCCAATGTA80681.0217158951031595No Hit
GAGACCTACATCAGGCCTTCCATCAATGCACCACTTTCTGTAGAACCATG79121.001960357220649No Hit
CTTAGTTTCTTATCATTTTGGCTTCTATACACTTTGGGGCAGCTGTAGAA78500.9941087972929847No Hit
TTACTTTACCTTTCCAATTTGCTGAAGAACCACCTTCAACATTTAAGTGT78140.9895498270124055No Hit
CAAATACCAGTGAACTTAAAGAATTCGACCCTTAGAAACTACATGTAGAA76560.9695410130031964No Hit
GAGACCTACATCAGGCCTTCATCAATGCACCACTTTCTGTAGAACCATGT75660.9581435873017481No Hit
AATTCTGCTGAATGGGTTGCTGGGTTGTGTCAAATATATAAGTTCATGTA74090.9382614113558884No Hit
CTTAGTTTCTTATCATTTTGGCTTCTTTACACTTTGGGGCAGCTGTAGAA71460.9049556006949897No Hit
GGAATCTCTGTGCTACCTTCCAGAATACTGAAACATTTGAGTTCCAAGAT67110.8498680431379899No Hit
CCCCCTCCTCTGTTGCTGCTTTACGTTTGGTGCTTGTAGAACCATGTCGT67050.8491082147578934No Hit
ATGACATGCTTCTTGAGCTTTCGGTGTTCTGAAACAACTGTAGAACCATG65890.83441819940936No Hit
CTGCACCCAGCCATAAGTAAAAGTGGCCAACTGCCCTGTAGAACCATGTC64910.8220076692011166No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT62440.7907280675538084No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG59410.7523567343589328No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC59070.7480510402050523No Hit
GAGGGGGTCGTGGGACTCTGGGCGCGGGAATCTGTAGAACCATGTCGTCA59030.7475444879516546No Hit
GGCACTCAGGAAAGTATCTCGTTCACTGACTGGACCCATGTAGAACCATG58400.7395662899606409No Hit
GGTTGAACTAAAACGACAATACAATGAAAGGTTTGTACCGGTGTAGAACC56550.7161382482409973No Hit
GTGTACTGCCCTCCTCCCACAGAGACTTGGCTCATCAGAATGTAGAACCA56040.7096797070101767No Hit
TGCCTGAATGTTCTTCACCACCAGCAACCTAAATGGAACTTGTAGAACCA55510.7029678896526571No Hit
TGCCTGAATGTTCTTCACCAGCAACCTAAATGGAACTTGTAGAACCATGT55360.7010683187024158No Hit
CTGCACCCAGCCATAAGTAAAGTGGCCAACTGCCCTGTAGAACCATGTCG55000.6965093484218364No Hit
ATGACATGCTTCTTGAGCTTTTCGGTGTTCTGAAACAACTGTAGAACCAT54960.6960027961684387No Hit
AATCAAATGAAAGAGATAAGAAAAACTGTGGAGCCCTTTGATGTAGAACC52510.6649764706478297No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA51370.6505397314259953No Hit
GAGTCCTCCTTCTGTGAGCAAACTGGCACCTGTAGAACCATGTCGTCAGT44940.5691114566923151No Hit
AAGAGGCTTCCAAATTGAGGGTTACTTCTTTTAAACACCATCTTAAAATG44920.5688581805656162No Hit
GTGGCAGCAATTTCATCTGTATCCTTTAAATTCAGCGATGTCTTGTAGAA43750.5540415271537336No Hit
ACACCGGATATCTGCTTGCCTCTGACCCCTGTAGAACCATGTCGTCAGTG43350.5489760046197566No Hit
GAGACCTACATCAGGCCTTCTTCCTACTCATGGAGATGTGTAGAACCATG43260.5478362620496118No Hit
GCAGATGTCTTTGAGTGAGTAGAGGGGGTGGGGGAGATGTAGAACCATGT42860.5427707395156347No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG42310.5358056460314163No Hit
ACTAGTATTCTGAGCTGTGTGTTCAGCCAGAGAAGTGAAAGCTGTAGAAC41880.530360209307391No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT40180.5088317385379889No Hit
CAGACAGGGACAAATCATCAGCTGGAGACACCAGGTCTGCCTGAGAAGCC39140.49566137994964865No Hit
CAGACTGTTAATACAGATTTCTCTCCAATCCAGACATATTTTGGTGTAGA38920.4928753425559614No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT38580.4885696484020809No Hit
CTCACCATTACCATTCCAAATATTCTTCCATTTTTTAAATATGTCATTTT36760.4655215208724856No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTACTTGGTCTGTCTAAGAACACAG36610.46362194992224426No Hit
CATGAGGGTAGTGTTGACAAAGATTAAGGTAGCAGATACATTGGACCACT36570.4631153976688465No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTAGAACACAGAGGAGAATTTATTA36320.4599494460851109No Hit
AACAGATATGCTATAGTACAGAAGGCAAAAGAAAAGTGATTACCTTGCTT35380.44804546813026497No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT35210.4458926210533247No Hit
ATGATCCATGGACATTTGACTGGTAGGTAAGTGGAGTGAAATTTAATGTA33540.4247440644739708No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT31850.403342231767918No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG31820.40296231757786977No Hit
TTTGAAACAGAAGCACATTAATATTTTATAAGCCATGTAGAACCATGTCG31180.3948574815235066No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG30390.384853074518902No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTGTCTGTCTAAGAACACAGAGGAG29200.36978314498032044No Hit
GAGACCTACATCAGGCCTTCATCCTACTCATGGAGATGTGTAGAACCATG28990.3671237456499825No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT25560.3236868899211298No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT25520.3231803376677321No Hit
TGCCTGAATGTTCTCCACCAGCAACCTAAATGGAACTTGTAGAACCATGT24760.31355584485317584No Hit
GGGATTCCATGATTCGATGTTCACTCCCCTTCCCACTTTGTGTAGAACCA24750.31342920678982644No Hit
GGGATTCCATGATTCGATATTCACTCCCCTTCCCACTTTGTGTAGAACCA23930.30304488559517356No Hit
AACATCTGAGGGGTTCTGGCATTTTCAGCAGGCTGTAGAACCATGTCGTC23320.29531996373085867No Hit
CCTGTCATGGGTATTATTTCTTTGCTTCTGGGGTAATAAATAACTGTAGT23320.29531996373085867No Hit
AGATTAGGGGTTTTGCAACCTGACCATGGTTCGCATCTCAGGTTTGTTCT22160.2806299483823254No Hit
GGTTGACCATCAAATATTCCTTCTCCAAGCAGATTCAAGGTGATCAGTTT22010.278730377432084No Hit
GCAGATGTCTTTGAGTGAGTAGAAGGGGTGGGGGAGATGTAGAACCATGT21360.2704989033143714No Hit
TGTGAGTGATAAAGGTGTTACTGTTGGTCTCATTAAGTGTGTAGAACCAT21350.270372265251022No Hit
CTTGGCCATAAGAAGCTCCCGTTCTTTCCTGGTCTTCTTGTAGAACCATG20480.259354753739622No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT20270.2566953544092841No Hit
AACATCTGAGGGGTTCAGTCATTTTCAGCAGGCTGTAGAACCATGTCGTC20010.2534027647621991No Hit
GAAAAGGGTCCAGATGATATGTTCTACTTAAAGCCACATTGCTGTGTAGA19910.25213638412870476No Hit
GCATGCCATTCAGAGGATTTTATCAAGAAAGCAGATTTGGTGTAGAACCA19630.24859051835492088No Hit
GGCACTCAGGAAAGTATCTCTTTGTGGGCATTAGTAGTGTAGAACCATGT18890.23921930166706348No Hit
TGGTCATTTGACAGTTCTGGGTTACTTCTTTTAAACACCATCTTAAAATG18500.2342804171964359No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT18210.23060791335930259No Hit
CACTCAAGAGCACAGGTACAGTTTTGTAAATTCATCTGGATAATTGTAGA18060.2287083424090612No Hit
ACACCGGATATCTGCTTCCTCTGACCCCTGTAGAACCATGTCGTCAGTGT17830.22579566695202447No Hit
GGCACTCAGGAAAGTATCTCGTTGTGGGCATTAGTAGTGTAGAACCATGT17530.2219965250515417No Hit
GGAGAGCTGTAAATTCTGGCTTTTTCAGAATGCATGTAGAACCATGTCGT16730.2118654799835877No Hit
CAAATACCAGTGAACTTAAAGAATTTGTCCCTTAGAAACTACATGTAGAA16660.21097901354014173No Hit
ATTGGTGATGATTCGATGGAGTGAATTTACCAGCACTGTAGAACCATGTC16650.21085237547679234No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA16060.20338072973917626No Hit
TATGGTCCTCAGAAAAGTGCACCAGATCATCTTCCATTTGTAATACTTCT16000.2026209013590797No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC14790.1872976956937993No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA14670.1857780389336062No Hit
TGCACGTACCACATTTAATTACCTTGTAGCAGAGACTTGGTCTTCTCTTG14640.1853981247435579No Hit
GGCCTTTTCCTTCAAACAGGATTCAAACTGTAGAACCATGTCGTCAGTGT13920.17628018418239932No Hit
TCTGGATCAGAGTCAGTGGTGTTACTGTTGGTCTCATTAAGTGTGTAGAA13830.1751404416122545No Hit
ACCTAGAGTCATTTTTATATGCTGCTTTACACTATTTGTAGAACCATGTC13330.16880853844478327No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTGATGTCTACAATATCACC12860.1628565494673603No Hit
CAGGACTAGCTCTAAGAAACCTGTACACTGCGACATGGCTTCTCTGATTT12840.16260327334066146No Hit
ACTAGTATTCTGAGCTGTGTGCTCAGCCAGTGTAGAACCATGTCGTCAGT12760.16159016883386604No Hit
TCACTGGAAAATTCTAACGTGAGGTGTGGCTGTAGAACCATGTCGTCAGT12580.15931068369357643No Hit
ACACTTCTGGGTAACTTTTTAGACTGGAATTAAGTGGAAGCCTGTAGAAC12460.15779102693338332No Hit
GATCCCCTGTGTGAGAGAAAAGAATGCTGAAATAACAATGCCTCAAGTGT11810.14955955281567068No Hit
ACTAGTATTCTGAGCTGTGTGCTCAGCCAGAGAAGTGAAAGCTGTAGAAC11560.14639360123193507No Hit
AGCTTCATCATACCTTTCACTAAGACAGCTAAGAGGGGAGGATGTAGAAC11020.13955514581106615No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTGACTTGGTCTGTCTAAGAACACA11020.13955514581106615No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTGCAGAT10470.13259005232684776No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTCTAAGAACACAGAGGAGAATTTA10380.13145030975670297No Hit
AGGGGTGGTAAATGTTAACTTATGTGAATGCGTGCTACATTCATGTAGAA10220.12942410074311214No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTGGTCTGTCTAAGAACACAGAGGA10000.12663806334942482No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT9910.12549832077927997No Hit
CTGGGTATGGTGGCACATCTGTAGAACCATGTCGTCAGTGTAGATCTCGG9340.11827995116836278No Hit
CAGCCAGACTGCCTTCCAGGTGAAGACGCTGTAGAACCATGTCGTCAGTG9170.11612710409142256No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT9070.11486072345792829No Hit
TGTGAGTGATAAAGGTGTCACTGTTGGTCTCATTAAGTGTGTAGAACCAT8540.10814890610040878No Hit
CTGAATGTGACAGCAAACAGGTGCATTTATCATGTAGAACCATGTCGTCA8520.10789562997370994No Hit
TGGGTAATGAGAACTCTGTCAGACTCTTGTAGGCCCCCGATGTAGAACCA8350.10574278289676971No Hit
CCTGACAGGTATGGTACAGCTTTCAGTGTAGAACCATGTCGTCAGTGTAG8300.10510959258002259No Hit
GAGTGTTCTGAAAGGAAATCTGGATAATTTCTTTTTTGAATGTGCACTGT8180.10358993581982949No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT8070.10219691712298581No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)