FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100597

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100597
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1082864
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC217832.011609952865734No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA197111.8202655181075371No Hit
AGTCTTCAGAATGCCAGAGATAATGACAGAAATTCCACTGTTGAAGACCA178791.6510845313908302No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC174291.6095280663130367No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAAAGACCAAGTC172421.592259046380709No Hit
CTCACGTAATTTTGTACTTTTTCTTCCTATTGAGCCTGTAGACCAGCAAC172331.591427917079153No Hit
TACTGAATGCAAAGGACACCACATTTAGCTGGGACACATAGACCAAGTCT171451.5833013194639403No Hit
ACATGTACATAGGGTTTATATATCATCAGCTATAAGTATCTTCTCTTGTC160461.4818111969739505No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT150551.3902946261026317No Hit
CCAAATCTGCTTTCTTGATAAAATCCTTTGAATGGCATGCAGACCAAGTC149641.3818909853869No Hit
TCTAAGCATAGCATTCAATTTTGGCCACTACAAATCATTCTCCTTAAGAC145801.346429468520516No Hit
TTAACTACAATTCCCAGCAAGCGGTCTCTGAGTCGAGACCAAGTCTCTGC145301.3418120835118723No Hit
AGTGGAACTGTTATTATCCCTCAAATGGCACAGCTGAGACCAAGTCTCTG131051.2102166107655254No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCACCACTGACTCTGATCCAG129251.193594024734408No Hit
CACTTAATGAGACCAACAGTAACACCACTGACTCTGATCCAGAAGACCAA123501.140494097135005No Hit
GGGGACGCTCTTGTATTATCTGTACACTGACTTATGGTATCAAATAATAA121581.122763338701813No Hit
TGGGGAAAAATATGCTTTGATCTACAGAAAAACATAGAAATCAGAGACCA117841.0882252988371577No Hit
ATCCTGTTCCAAGTCAAGACCACGGCAGAACTGTCAAATGACCAAGACCA107170.9896903027527002No Hit
TGGGTCCAGTCAGTGAACGAGATACTTTCCTGAGTGCCAGACCAAGTCTC105670.975838147726769No Hit
AGTGGAACTGTTATTATCCCATCAAATGGCACAGCTGAGACCAAGTCTCT103870.9592155616956515No Hit
TGGGGAAAAATATGCTTTGAGCTACAGAAAAACATAGAAATCAGAGACCA99570.9195060506213153No Hit
GAAAGTGGTGCATTGATGAAGGCCTGATGTAGGTCTCAGACCAAGTCTCT98150.906392677196767No Hit
TCAAAGGGCTCCACTGGTTTTTCTTATCTCTTTCATTTGATTAGACCAAG96180.8882001802627107No Hit
GCTGCCCCAAAGTGTATAGAAGCCAAAATGATAAGAAACTAAGAGACCAA93390.8624351719144786No Hit
GGCTTTATGCAAGCCTTCAACATACACTGACTTATGGTATCAAATAATAA93360.86215812881396No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC93250.8611423041120584No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA91960.8492294507897575No Hit
GAAAGTGGTGCATTGATGGAAGGCCTGATGTAGGTCTCAGACCAAGTCTC89860.8298364337534537No Hit
TGCAGATTTCATGTGCCTAGAAGTTACGGTAGCAAAGGCCAATCAAGTGA89540.8268813073479218No Hit
GCTGCCCCAAAGTGTAAAGAAGCCAAAATGATAAGAAACTAAGAGACCAA87300.8061954225091977No Hit
GGGGTCAGAGGCAAGCAGATATCCGGTGTAGACCAAGTCTCTGCTACCGT86520.7989923018957136No Hit
GGGAAAAGAACAGGCTTCACCTATCATCGTTTTCCAAGTTATTTTTGAGA86150.7955754369893172No Hit
AGTTCCATTTAGGTTGCTGGTGAAGAACATTCAGGCAAGACCAAGTCTCT84910.7841243221678806No Hit
TGTGTCATGTAATCAAATAGTAGATGTGCACTGACGACATTGATTAGACT84120.7768288538542236No Hit
TGTAGTTTCTAAGGGTCGAATTCTTTAAGTTCACTGGTATTTGAGACCAA82240.7594674862217232No Hit
ATTATCCAGATGAATTTACAAAACTGTACATGTGCTCTTGAGTGAGACCA81500.7526337564089304No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCACACTGACTTATGGTATCAAATA79740.7363805611785044No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA77730.7178186734437566No Hit
ATCCTGTTCCAAGTCAAGACCACAGCAGAACTGTCAAATGACCAAGACCA75370.6960246162029581No Hit
GGGATAAAAGCACAAAGGTCTGCTACCGTAACCTTCAGAGCTAGGGAAAA72600.6704443032550718No Hit
TGCAATTCAGTACAATTAGGTGGGACACTGACGACATGGTTCAGCTTTCG71470.6600090131355368No Hit
TGTCTTTTCTTCCCTAGTATGTACACTGACGACATTGATTAGACTGTTGA70930.6550222373262017No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTAACG70730.6531752833227441No Hit
TCAAAGGGCTCCACAGTTTTTCTTATCTCTTTCATTTGATTAGACCAAGT70530.6513283293192866No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTAACGCGGA69060.637753217393874No Hit
GGGCAGTTGGCCACTTTTACTTATGGCTGGGTGCAGAGACCAAGTCTCTG68890.6361833064909351No Hit
GGTGCCAGTTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACCG67260.6211306313627566No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTAACGC66210.6114341228446046No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAAA65420.6041386545309476No Hit
CTCATTCTTTCCTTGATTTTCTTCCTCCTACGGTAGCAAATACTGAGCAT65190.6020146574269715No Hit
GGGCAGTTGGCCACTTTACTTATGGCTGGGTGCAGAGACCAAGTCTCTGC64640.5969355339174632No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA62440.5766190398794309No Hit
TTGAAACTACAAATGAAAGAGCTCATACGGTAGCAGAAGCCTTTTTAGCC62210.5744950427754547No Hit
CTCCCAAAATTCAAAATCCTCACACTGACGACATGTTTCTTCTATAACCC60650.5600888015484863No Hit
GTGCACATTCAAAAAAGAAATTATCCAGATTTCCTTTCAGAACACTCAGA59700.5513157700320631No Hit
TGAATGTAGCACGCATTCACATAGGTTAACATTTACCACCCCTAGACCAA59680.5511310746317174No Hit
TGAACTTATATATTTGACACAACCCAGCAACCCATTCAGCAGAATTAGAC59090.5456825603215177No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAAC58170.5371865719056133No Hit
GTATAATGATGAGCATTTTGGCTACCGTACACATAGGTTAACATTTACCA56470.5214874628762245No Hit
AGTTCCATTTAGGTTGCTGGTGGAGAACATTCAGGCAAGACCAAGTCTCT55450.5120679974585913No Hit
AGACATCGCTGAATTTAAAGGATACAGATGAAATTGCTGCCACAGACCAA55160.5093899141535779No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTAAAATGAAATACTTTGAGAA54470.5030179228416496No Hit
TCTCCCCCACCCCTTCTACTCACTCAAAGACATCTGCAGACCAAGTCTCT52630.4860259460098406No Hit
AATGGTTTTACCAAGGAAGAATCCCCACCACAATACCAAGACCAAGTCTC52520.4850101213079389No Hit
GGGGTCAGAGGAAGCAGATATCCGGTGTAGACCAAGTCTCTGCTACCGTA52370.4836249058053458No Hit
TTACCCAGTACCATCAATGCTGTTAATGAGAGAAGTTTCTGAGAGGTAGA52170.4817779518018883No Hit
GTTGTTTCAGAACACCGAAAGCTCAAGAAGCATGTCATAGACCAAGTCTC52060.4807621270999867No Hit
GCCTGCTGAAAATGCCAGAACCCCTCAGATGTTAGACCAAGTCTCTGCTA51150.4723584863842551No Hit
AAGGGAAAAGGATGGATTAGGGTTACGGTAGCACAGCAAGTGGAAAATCT51110.47198909558356356No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT50080.4624772824657575No Hit
TTCTGATGAGCCAAGTCTCTGTGGGAGGAGGGCAGTACACAGACCAAGTC49660.45859867905849677No Hit
CTGTGGTGCCACCTAAGCTCTGCTACCGTACAAGTGGAAAGGTGGAGTCA49250.45481242335140887No Hit
GCCTGCTGAAAATGACTGAACCCCTCAGATGTTAGACCAAGTCTCTGCTA48290.44594704413481284No Hit
GTTGTTTCAGAACACCGAAAAGCTCAAGAAGCATGTCATAGACCAAGTCT47330.4370816649182169No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAA44410.4101161364677374No Hit
AATGGTTTTACCAAGGAAGGATCCCCACCACAATACCAAGACCAAGTCTC42610.39349355043661993No Hit
TGTCCTGCTTGCTTACCTCGCAGTTTATTTGAGGAAAAGAGAAGCAAGAG42590.39330885503627416No Hit
TCAAATCATCCATTGCTTGGTACGGTAGCAGAAAGTCAGTCCCATGGAGA42200.38970729472953203No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTAACGCGG41920.38712155912469154No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC41570.38388938961864094No Hit
CTCCTTTCTGGACGCTTTTGCTACCGTATGAGGCATAACTGCACCCTTAG40040.3697601914921911No Hit
TCTCCCCCACCCCCTCTACTCACTCAAAGACATCTGCAGACCAAGTCTCT39580.36551219728423884No Hit
GCTTAGATTCCTCTTTGGAGTCAGTGTACCTCTCACAATATTAAATGCAC38310.3537840393622837No Hit
GTGCTGGTAAATTCACTCCATCGAATCATCACCAATAGACCAAGTCTCTG37110.3427023153415387No Hit
CAGTAGAGGCTAAGGATTCAGGTACACTGACGACATGGTGGTTGATCTTA35800.3306047666188921No Hit
AAACTATAAGATAAGGAATCCAGCAAAGGCCAATCAAGTGAAGAAAGACC35780.33042007121854633No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTAACGCG35170.324786861508001No Hit
CCAATTTTGGTAAGCTGCCCATCCAAGCAGAAGCCTTTTTAGCCCTAGAC34890.3222011259031605No Hit
CCAAATCTGCTTTCTTGATAAAATCCTCTGAATGGCATGCAGACCAAGTC34600.3195230425981471No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC34470.31832252249589976No Hit
GAAGTATTACAAATGGAAGATGATCTGGTGCACTTTTCTGAGGACCATAA32880.30363923816841265No Hit
CTACTAATGCCCACAACGAGATACTTTCCTGAGTGCCAGACCAAGTCTCT32470.29985298246132475No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTAACGCGGACTAATC32430.29948359166063326No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT32010.29560498825337256No Hit
CTCCTCCACAGCTCGCTACCGTACATCAGATCCTTACCTGTCAGGAGACC31950.2950509020523353No Hit
CAAAGTGGGAAGGGGAGTGAACATCGAATCATGGAATCCCAGACCAAGTC31090.28710899983746807No Hit
GACTACAGATGTGCCACCATACCAACTGCTCTGGAACATGTTAGACCAAG30270.2795364884232923No Hit
CAAAGTGGGAAGGGGAGTGAATATCGAATCATGGAATCCCAGACCAAGTC28810.26605372419805257No Hit
TGTAGTTTCTAAGGGACAAATTCTTTAAGTTCACTGGTATTTGAGACCAA28590.26402207479424933No Hit
CATCTCCATGAGTAGGAAGAAGGCCTGATGTAGGTCTCAGACCAAGTCTC28050.25903529898491406No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTAACGC28010.2586659081842226No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG27780.2565419110802465No Hit
CTACTAATGCCCACAAAGAGATACTTTCCTGAGTGCCAGACCAAGTCTCT27760.2563572156799007No Hit
CACCTCGAACACCCAGGCACACTGACGACATGTTTCTTCTATAACCCACA27540.2543255662760974No Hit
AGCACCAAACGTAAAGCAGCAACAGAGGAGGGGGAGACCAAGTCTCTGCT27310.25220156917212133No Hit
AATAATGAGTTTGGTCATCTGAACTTTACACTGACGACAGGTTTCTTAGA26730.2468454025620946No Hit
GGAAACACTGATGGGCCTGAAAGTCACACCAGAACGAAAAGAGCAGCCGT26050.24056575895033908No Hit
GCCTACCAAAATCAGAGAAGCCATCATCAGTCAGCTAACATGTAGACCAA24980.23068455503184152No Hit
AATCTCCAAGGAAGTTGTACCGTCTTAGACCAAGTCTCTGCTACCGTAAC24720.22828351482734674No Hit
GAGAAAGCCCCCCTACTGCTACCGTAAGAGTCACAGTCACAGGTAGGTTA22800.21055275639415477No Hit
GCCTACCAAAATCAGAGAAACCATCATCAGTCAGCTAACATGTAGACCAA22490.20768997768879563No Hit
CAGCAATGTGGCTTTAAGTAGAACATATCATCTGGACCCTTTTCAGACCA22320.20612006678585676No Hit
AGTAAGGGTGGCATCAATCTTTACACTGACGACATGGTTCTACAAGTAAG21760.20094859557617575No Hit
CTGAAATGTGAACCAATATTAGCAAGACCAAGTCTCTGCTACCGTAACGC21580.19928633697306403No Hit
TGCAATTCAGTACAATTAGGTGGAAAGGAGGTCTCAATCTGAAGACCAAG21120.19503834276511178No Hit
TGGCTTATAAAATATTAATGTGCTTCTGTTTCAAAAGACCAAGTCTCTGC20940.19337608416200003No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC20660.19079034855715954No Hit
GAGAAAGCCCCCCTACTGCTACCGTACAGCTCTGAACATCTAGGGCAAGA20570.18995921925560366No Hit
CGGCCCCTTCCGCTTTACCGACATGGTTCTACAGATTCCCGCGCCCAGAG20360.18801991755197328No Hit
TGATAAATGCACCTGTTTGCTGTCACATTCAGAGACCAAGTCTCTGCTAC19680.1817402739402178No Hit
AAATAACCTAAGGGATTTGCTTTGTGACATGGTTCTACAAAATAACCTAA19280.1780463659333028No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTAACGCG19130.17666115043070968No Hit
GGGGACGCTCTTGTATTATCTGTCTTGGTCTACGTTTTTAGTTGAAGTAA19050.17592236882932666No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCAAA18660.17232080852258455No Hit
ATTATCCAGATGAATTTACAAAACTGTACCTGTGCTCTTGAGTGAGACCA18370.16964272521757118No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGCAGTGTTCATCATTGA18240.1684422051153238No Hit
GGCTTCCACTTAATTCCAGTCTAAAAAGTTACCCAGAAGTGTAGACCAAG17790.16428655860754443No Hit
AAGTCCTCCATTTCTGTATCCATGCGAGACCAAGTCTCTGCTACCGTAAC17600.16253195230425982No Hit
TGCATTCTGAAAAAGCCAGAATTTACAGCTCTCCAGACCAAGTCTCTGCT17460.16123908450183957No Hit
TGAATTCTCCTCAGATGACTCCATTTAAAGACCAAGTCTCTGCTACCGTA17280.15957682589872782No Hit
AAAAGCAGTGGTCCAATGTATCTGCTACCGTATCCCACAGACTGATATGG17180.15865334889699909No Hit
CATCTCCATGAGTAGGATGAAGGCCTGATGTAGGTCTCAGACCAAGTCTC16240.14997266508074883No Hit
AACAATCGACAGGCTAGAAGTTGAGACCAAGTCTCTGCTACCGTAACGCG15410.14230780596640022No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG15040.13889094106000383No Hit
ATCTCCTGGTTATTCTCAGTTTTCTTTACCAGTGCAAGACCAAGTCTCTG14840.13704398705654636No Hit
AGCAATCTGCTTGTGTGTGCGTGAGACCAAGTCTCTGCTACCGTAACGCG14050.12974851874288923No Hit
AGAGGAACGTTACACACCAGCAAATGATCAGCCTTGCAAAGACCAAGTCT13960.12891738944133335No Hit
GCTTTCACTTCTCTGGCTGAACACACAGCTCAGAATACTAGTAGACCAAG13560.12522348143441836No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCATCCAAAAGACCTATATCCAGG13500.12466939523338111No Hit
TTAAATTTCACTCCACTTACCTACCAGTCAAATGTCCATGGATCATAGAC13490.12457704753320822No Hit
ATTTAACACCTAGCCAAAAGGCAGACCAAGTCTCTGCTACCGTAACGCGG13470.12439235213286248No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCCGTAA13460.12430000443268961No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTCCAATCAA13190.121806616528022No Hit
CCTCAGGGCCTCAGAGAAAGGGTGGGGAAACTTGAAAGTAGACCAAGTCT13070.12069844412594749No Hit
TCTATTGTGATGGCCACGTTAGACCAAGTCTCTGCTACCGTAACGCGGAC12560.11598871141713088No Hit
CAGCAATGTGGCTTTAATTAGAACATATCATCTGGACCCTTTTCAGACCA12540.11580401601678511No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTAACGCGG12400.11451114821436487No Hit
CCAAAATATGTCTGGATTGGAGAGAAATCTGTATTAACAGTCTGAGACCA12260.11321828041194462No Hit
AGTAAGGGTGGCATCAAAAATTGGTTTTCTTTGCAGACCAAGTCTCTGCT12150.11220245571004299No Hit
TGTGCCTCCAAACTTACAGGTACACTGACGACATGGTTCTACATGTGCTC11620.10730802760088062No Hit
CACAAAGGTTTTTATAAGTTCTGTGGATCTAGACCAAGTCTCTGCTACCG11590.107030984500362No Hit
GATGTGCCACCATACCCAGAGACCAAGTCTCTGCTACCGTAACGCGGACT11440.10564576899776887No Hit
GCGTCTTCACCTGGAAGGCAGTCTGGCTGAGACCAAGTCTCTGCTACCGT11230.1037064672941385No Hit
CTTAAATGTTGAAGGTGGTTCTTCAGCAAATTGGAAAGGTAAAGTAAAGA11140.10287533799258264No Hit
GCCACACCTCACGTTAGAATTTTCCAGTGAAGACCAAGTCTCTGCTACCG10980.10139777478981662No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)