FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1082864
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG205271.895621241448603No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT193751.7892366908494513No Hit
TCAACAGTGGAATTTCTGTCATTATCTCTGGCATTCTGAAGACTTGTAGA175571.6213485719351644No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA172281.5909661785782885No Hit
CCCCCTCCTCTGTTGCTGGTCTACAGGCTCAATAGGAAGAAAAAGTACAA169411.5644623886286735No Hit
TTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA169251.5629848254259078No Hit
ATGTGTCCCAGCTAAATGTGGTGTCCTTTGCATTCAGTATGTAGAACCAT168801.5588291789181281No Hit
CCATGGACAAGAGAAGATACTTATAGCTGATGATATATAAACCCTATGTA159811.4758085964627137No Hit
GCATGCCATTCAAAGGATTTTATCAAGAAAGCAGATTTGGTGTAGAACCA147961.3663765717578569No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC147841.3652683993557826No Hit
CGACTCAGAGACCGCTTGCTGGGAATTGTAGTTAATGTAGAACCATGTCG144101.3307303594911273No Hit
TAAGGAGAATGATTTGTAGTGGCCAAAATTGAATGCTATGCTTAGATGTA143641.326482365283175No Hit
CAGCTGTGCCATTTGAGGGATAATAACAGTTCCACTTGTAGAACCATGTC130091.2013512315489294No Hit
TCTGGATCAGAGTCAGTGGTGCCTTTCTTCATCCATATTACTTTATACTC125171.1559161630638752No Hit
TCTGGATCAGAGTCAGTGGTGTTACTGTTGGTCTCATTAAGTGTGTAGAA121681.1236868157035416No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTACAGATAATACAAG119321.1018927584627434No Hit
CTGATTTCTATGTTTTTCTGTAGATCAAAGCATATTTTTCCCCATGTAGA115471.0663388938961864No Hit
GGCACTCAGGAAAGTATCTCGTTCACTGACTGGACCCATGTAGAACCATG105220.9716825012189897No Hit
TGGTCATTTGACAGTTCTGCCGTGGTCTTGACTTGGAACAGGATTGTAGA104580.9657722484079256No Hit
CAGCTGTGCCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGT102680.9482261853750794No Hit
CTGATTTCTATGTTTTTCTGTAGCTCAAAGCATATTTTTCCCCATGTAGA97500.9003900766855302No Hit
GAGACCTACATCAGGCCTTCATCAATGCACCACTTTCTGTAGAACCATGT96620.8922634790703172No Hit
AATCAAATGAAAGAGATAAGAAAAACCAGTGGAGCCCTTTGATGTAGAAC95940.8859838354585617No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG92850.8574483961051433No Hit
CTTAGTTTCTTATCATTTTGGCTTCTATACACTTTGGGGCAGCTGTAGAA91510.8450738042819782No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTATGTTGAAGGCTTG91380.8438732841797308No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT90750.8380553790688398No Hit
GAGACCTACATCAGGCCTTCCATCAATGCACCACTTTCTGTAGAACCATG88980.8217098361382408No Hit
TTCTTCACTTGATTGGCCTTTGCTACCGTAACTTCTAGGCACATGAAATC86520.7989923018957136No Hit
ACACCGGATATCTGCTTGCCTCTGACCCCTGTAGAACCATGTCGTCAGTG86310.7970530001920831No Hit
CTTAGTTTCTTATCATTTTGGCTTCTTTACACTTTGGGGCAGCTGTAGAA85920.7934514398853411No Hit
CAAAAATAACTTGGAAAACGATGATAGGTGAAGCCTGTTCTTTTCCCTGT85500.7895728364780804No Hit
TGCCTGAATGTTCTTCACCAGCAACCTAAATGGAACTTGTAGAACCATGT83290.769163994739875No Hit
CAAATACCAGTGAACTTAAAGAATTCGACCCTTAGAAACTACATGTAGAA81210.749955673103917No Hit
CACTCAAGAGCACATGTACAGTTTTGTAAATTCATCTGGATAATTGTAGA80980.7478316759999409No Hit
AGCAATTTCAACAGTCTAATCAATGTCGTCAGTGCACATCTACTATTTGA80380.7422908139895684No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTGGACACTACAAAGG79210.7314861330693421No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT77250.7133859838354586No Hit
TGGTCATTTGACAGTTCTGCTGTGGTCTTGACTTGGAACAGGATTGTAGA73370.6775550761683832No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA70920.6549298896260287No Hit
AATCAAATGAAAGAGATAAGAAAAACTGTGGAGCCCTTTGATGTAGAACC69950.64597216270926No Hit
GCTTTCAAAACGAAAGCTGAACCATGTCGTCAGTGTCCCACCTAATTGTA69640.6431093840039007No Hit
AGCAATTTCAACAGTCTAATCAATGTCGTCAGTGTACATACTAGGGAAGA68480.6323970507838472No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC68260.630365401380044No Hit
ACTTTTTCCCTAGCTCTGAAGGTTACGGTAGCAGACCTTTGTGCTTTTAT67810.6262097548722647No Hit
CTGCACCCAGCCATAAGTAAAAGTGGCCAACTGCCCTGTAGAACCATGTC67760.6257480163714002No Hit
GAGTCCTCCTTCTGTGAGCAAACTGGCACCTGTAGAACCATGTCGTCAGT67400.6224234991651768No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT65800.6076478671375168No Hit
CTGCACCCAGCCATAAGTAAAGTGGCCAACTGCCCTGTAGAACCATGTCG63650.5877931116003486No Hit
AGTGAACTTGATGCTCAGTATTTGCTACCGTAGGAGGAAGAAAATCAAGG63240.5840068558932608No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT61280.5659067066593774No Hit
AGGGCTAAAAAGGCTTCTGCTACCGTATGAGCTCTTTCATTTGTAGTTTC59800.5522392470337919No Hit
GAGTGTTCTGAAAGGAAATCTGGATAATTTCTTTTTTGAATGTGCACTGT58750.5425427385156399No Hit
TACTGGTGTGGGTTATAGAAGAAACATGTCGTCAGTGTGAGGATTTTGAA58710.5421733477149485No Hit
AGGGGTGGTAAATGTTAACCTATGTGAATGCGTGCTACATTCATGTAGAA58470.5399570029107995No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG57810.5338620546993897No Hit
AATTCTGCTGAATGGGTTGCTGGGTTGTGTCAAATATATAAGTTCATGTA57370.5297987558917833No Hit
TGCCTGAATGTTCTCCACCAGCAACCTAAATGGAACTTGTAGAACCATGT55070.508558784852022No Hit
GTGGCAGCAATTTCATCTGTATCCTTTAAATTCAGCGATGTCTTGTAGAA54760.505696006146663No Hit
AGGGGTGGTAAATGTTAACCTATGTGTACGGTAGCCAAAATGCTCATCAT53910.4978464516319686No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTAGAACACAGAGGAGAATTTATTA53800.4968306269300669No Hit
GCAGATGTCTTTGAGTGAGTAGAAGGGGTGGGGGAGATGTAGAACCATGT52250.4825167334032713No Hit
ACACCGGATATCTGCTTCCTCTGACCCCTGTAGAACCATGTCGTCAGTGT52190.4819626472022341No Hit
ACCTCTCAGAAACTTCTCTCATTAACAGCATTGATGGTACTGGGTAATGT51550.47605239439117003No Hit
ATGACATGCTTCTTGAGCTTTCGGTGTTCTGAAACAACTGTAGAACCATG51360.4742977880878855No Hit
TGGTATTGTGGTGGGGATTCTTCCTTGGTAAAACCATTTGTAGAACCATG51190.4727278771849466No Hit
AACATCTGAGGGGTTCTGGCATTTTCAGCAGGCTGTAGAACCATGTCGTC50250.4640471933686964No Hit
ACAGATTTTCCACTTGCTGTGCTACCGTAACCCTAATCCATCCTTTTCCC50190.4634931071676591No Hit
GTGTACTGCCCTCCTCCCACAGAGACTTGGCTCATCAGAATGTAGAACCA49130.4537042509493343No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT49130.4537042509493343No Hit
CTCTGACTCCACCTTTCCACTTGTACGGTAGCAGAGCTTAGGTGGCACCA48330.4463164349355043No Hit
AACATCTGAGGGGTTCAGTCATTTTCAGCAGGCTGTAGAACCATGTCGTC47670.4402214867240946No Hit
ATGACATGCTTCTTGAGCTTTTCGGTGTTCTGAAACAACTGTAGAACCAT46570.4300632397050784No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA44400.4100237887675645No Hit
GCAGATGTCTTTGAGTGAGTAGAGGGGGTGGGGGAGATGTAGAACCATGT43090.3979262400449179No Hit
GCCTCTTGCTTCTCTTTTCCTCAAATAAACTGCGAGGTAAGCAAGCAGGA42030.3881373838265932No Hit
CCATGGGACTGACTTTCTGCTACCGTACCAAGCAATGGATGATTTGATGT41890.38684451602417297No Hit
TGGTATTGTGGTGGGGATCCTTCCTTGGTAAAACCATTTGTAGAACCATG41720.38527460512123407No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT41600.38416643271915957No Hit
AAGGGTGCAGTTATGCCTCATACGGTAGCAAAAGCGTCCAGAAAGGAGTG39310.3630188093795712No Hit
GCAAAGAAAACCAATTTTTGATGCCACCCTTACTTGTAGAACCATGTCGT37120.3427946630417116No Hit
TGAAGTGCATTTAATATTGTGAGAGGTACACTGACTCCAAAGAGGAATCT36910.3408553613380812No Hit
ATTGGTGATGATTCGATGGAGTGAATTTACCAGCACTGTAGAACCATGTC36440.3365150194299561No Hit
ATGTTACCTTAAGATCAACCACCATGTCGTCAGTGTACCTGAATCCTTAG35940.33189763442131237No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC35030.32349399370558074No Hit
TTCTTCACTTGATTGGCCTTTGCTGGATTCCTTATCTTATAGTTTTGTAG34740.3208159104005674No Hit
GCATGCCATTCAGAGGATTTTATCAAGAAAGCAGATTTGGTGTAGAACCA34320.31693730699330663No Hit
AGGGCTAAAAAGGCTTCTGCTTGGATGGGCAGCTTACCAAAATTGGTGTA34120.31509035298984917No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG34070.31462861448898477No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT34030.31425922368829323No Hit
TATGGTCCTCAGAAAAGTGCACCAGATCATCTTCCATTTGTAATACTTCT32200.29735959455665717No Hit
GGCACTCAGGAAAGTATCTCGTTGTGGGCATTAGTAGTGTAGAACCATGT32100.29643611755492844No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT32060.2960667267542369No Hit
CCTGACAGGTAAGGATCTGATGTACGGTAGCGAGCTGTGGAGGAGTGTAG31400.2899717785428272No Hit
GGGATTCCATGATTCGATGTTCACTCCCCTTCCCACTTTGTGTAGAACCA30770.28415387343193604No Hit
AACATGTTCCAGAGCAGTTGGTATGGTGGCACATCTGTAGTCTGTAGAAC29720.2744573649137842No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG29170.2693782414042761No Hit
GGGATTCCATGATTCGATATTCACTCCCCTTCCCACTTTGTGTAGAACCA28820.2661460718982254No Hit
CAAATACCAGTGAACTTAAAGAATTTGTCCCTTAGAAACTACATGTAGAA28480.2630062500923477No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT27920.2578347788826667No Hit
GAGACCTACATCAGGCCTTCTTCCTACTCATGGAGATGTGTAGAACCATG27800.2567266064805922No Hit
GGCACTCAGGAAAGTATCTCTTTGTGGGCATTAGTAGTGTAGAACCATGT27580.25469495707678896No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT27180.25100104906987397No Hit
CCCCCTCCTCTGTTGCTGCTTTACGTTTGGTGCTTGTAGAACCATGTCGT26960.24896939966607073No Hit
TACTGGTGTGGGTTATAGAAGAAACATGTCGTCAGTGTGCCTGGGTGTTC26620.24582957786019297No Hit
CAGGACTAGCTCTAAGAAACCTGTCGTCAGTGTAAAGTTCAGATGACCAA26610.24573723016002008No Hit
GACGGCTGCTCTTTTCGTTCTGGTGTGACTTTCAGGCCCATCAGTGTTTC25760.23788767564532576No Hit
TCATTCAGAAAACAAACAGAGCACATGTAGAACCATGTCGTCAGTGTACC25700.23733358944428845No Hit
AAGACGGTACAACTTCCTTGGAGATTTGTAGAACCATGTCGTCAGTGTAG24650.2276370809261366No Hit
ACATGTTAGCTGACTGATGATGGCTTCTCTGATTTTGGTAGGCTGTAGAA24620.227360037825618No Hit
AACCTACCTGTGACTGTGACTCTTACGGTAGCAGTAGGGGGGCTTTCTCT22300.20593537138551105No Hit
ACATGTTAGCTGACTGATGATGGTTTCTCTGATTTTGGTAGGCTGTAGAA22110.20418076508222638No Hit
GAAAAGGGTCCAGATGATATGTTCTACTTAAAGCCACATTGCTGTGTAGA22000.2031649403803248No Hit
TGCTAATATTGGTTCACATTTCAGTGTAGAACCATGTCGTCAGTGTAGAT21360.1972546875692608No Hit
TCAGATTGAGACCTCCTTTCCACCTAATTGTACTGAATTGCATGTAGAAC20780.19189852095923401No Hit
TTTGAAACAGAAGCACATTAATATTTTATAAGCCATGTAGAACCATGTCG20710.1912520870580239No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG20280.1872811359505903No Hit
CTTGCCCTAGATGTTCAGAGCTGTACGGTAGCAGTAGGGGGGCTTTCTCT20150.1860806158483429No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGGTAAAGCGGAAGGGGCCGTG20050.18515713884661417No Hit
CTGAATGTGACAGCAAACAGGTGCATTTATCATGTAGAACCATGTCGTCA19480.17989331993676028No Hit
GCCCACTCTGGGTCCTTAGGTTATTTTGTAGAACCATGTCACAAAGCAAA19330.17850810443416718No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC19240.1776769751326113No Hit
TCTCCTTGCCATCATTACTTCAACTAAAAACGTAGACCAAGACAGATAAT18660.17232080852258455No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTGAGACTTGGTCTGTCTAAGAACA18420.17010446371843554No Hit
CACTCAAGAGCACAGGTACAGTTTTGTAAATTCATCTGGATAATTGTAGA18110.16724168501307643No Hit
CAATGATGAACACTGCGGGCCTTTCTTCATCCATATTACTTTATACTCCT18090.1670569896127307No Hit
GGAGAGCTGTAAATTCTGGCTTTTTCAGAATGCATGTAGAACCATGTCGT17710.16354777700616144No Hit
ACACTTCTGGGTAACTTTTTAGACTGGAATTAAGTGGAAGCCTGTAGAAC17530.1618855184030497No Hit
CGCATGGATACAGAAATGGAGGACTTTGTAGAACCATGTCGTCAGTGTAG17510.16170082300270394No Hit
TTAAATGGAGTCATCTGAGGAGAATTCATGTAGAACCATGTCGTCAGTGT17060.15754517649492458No Hit
CAGCCATATCAGTCTGTGGGATACGGTAGCAGATACATTGGACCACTGCT16750.15468239778956547No Hit
GAGACCTACATCAGGCCTTCATCCTACTCATGGAGATGTGTAGAACCATG16040.14812571107729133No Hit
CAACTTCTAGCCTGTCGATTGTTTGTAGAACCATGTCGTCAGTGTAGATC15120.13962972266138685No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT14750.13621285775499045No Hit
TGCACTGGTAAAGAAAACTGAGAATAACCAGGAGATTGTAGAACCATGTC14490.13381181755049573No Hit
CACGCACACACAAGCAGATTGCTTGTAGAACCATGTCGTCAGTGTAGATC13980.1291020848416791No Hit
CCAGATCCTTAACATTGGTCCGTATTGTAGAACCATGTCGTCAGTGTACT13660.1261469584361471No Hit
TTGCAAGGCTGATCATTTGCTGGTGTGTAACGTTCCTCTTGTAGAACCAT13660.1261469584361471No Hit
ATGATCCATGGACATTTGACTGGTAGGTAAGTGGAGTGAAATTTAATGTA13360.12337652743096086No Hit
ACTAGTATTCTGAGCTGTGTGTTCAGCCAGAGAAGTGAAAGCTGTAGAAC13350.12328417973078798No Hit
GCCTTTTGGCTAGGTGTTAAATTGTAGAACCATGTCGTCAGTGTAGATCT13210.12199131192836773No Hit
ACCTGGATATAGGTCTTTTGGATGGTACGGTAGCAGTAGGGGGGCTTTCT13080.12079079182612036No Hit
ACTTTCAAGTTTCCCCACCCTTTCTCTGAGGCCCTGAGGTGTAGAACCAT12810.11829740392145276No Hit
AGTTACAGCTACTGCTTGATTGGAGCAGAGACTTGGTCTTCTCTTGGTAT12440.11488053901505636No Hit
AACGTGGCCATCACAATAGATGTAGAACCATGTCGTCAGTGTAGATCTCG12340.11395706201332763No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT12280.11340297581229038No Hit
GAAAAGGGTCCAGATGATATGTTCTAATTAAAGCCACATTGCTGTGTAGA12130.11201776030969725No Hit
CAGACTGTTAATACAGATTTCTCTCCAATCCAGACATATTTTGGTGTAGA12120.11192541260952438No Hit
AGATCCACAGAACTTATAAAAACCTTTGTGTGTAGAACCATGTCGTCAGT11590.107030984500362No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTAGCAGAGACTTGGTCTGTCTAAG11420.10546107359742313No Hit
CTGGGTATGGTGGCACATCTGTAGAACCATGTCGTCAGTGTAGATCTCGG11250.10389116269448426No Hit
CAGCCAGACTGCCTTCCAGGTGAAGACGCTGTAGAACCATGTCGTCAGTG11190.10333707649344699No Hit
TCACTGGAAAATTCTAACGTGAGGTGTGGCTGTAGAACCATGTCGTCAGT10910.1007513408886065No Hit
TTACTTTACCTTTCCAATTTGCTGAAGAACCACCTTCAACATTTAAGTGT10890.10056664548826076No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)