FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100603

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100603
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences821670
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC194252.3640877724633977No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA180232.193459661421252No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC177412.1591393138364547No Hit
TACTGAATGCAAAGGACACCACATTTAGCTGGGACACATAGACCAAGTCT164972.007740333710614No Hit
CCAAATCTGCTTTCTTGATAAAATCCTTTGAATGGCATGCAGACCAAGTC161201.9618581669039883No Hit
CATTGGTGCTAAAAGTTTCTTGGACACTGACACAATGGCAACAGTTCTGA160651.9551644820913503No Hit
TTAACTACAATTCCCAGCAAGCGGTCTCTGAGTCGAGACCAAGTCTCTGC160221.9499312376014704No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTA159811.9449413998320495No Hit
TGAACTTATATATTTGACACAACCCAGCAACCCATTCAGCAGAATTAGAC159611.942507332627454No Hit
TCTAAGCATAGCATTCAATTTTGGCCACTACAAATCATTCTCCTTAAGAC151671.8458748646050116No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT149041.8138668808645806No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT143261.7435223386517702No Hit
AGAGGAACGTTACACACCAGCAAATGATCAGCCTTGCAAAGACCAAGTCT134091.6319203573210657No Hit
CTTGCCCCTTTCGTCTATTTGTACACTGACTTATGGTATCAAATAATAAA129711.5786142855404237No Hit
CACTTAATGAGACCAACAGTAACACACCACTGACTCTGATCCAGAAGACC129631.5776406586585856No Hit
TTAAATTTCACTCCACTTACCTACCAGTCAAATGTCCATGGATCATAGAC125151.523117553275646No Hit
AGTGGAACTGTTATTATCCCTCAAATGGCACAGCTGAGACCAAGTCTCTG123621.5044969391604903No Hit
TGGGGAAAAATATGCTTTGATCTACAGAAAAACATAGAAATCAGAGACCA113321.3791424781238208No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCCAAAACTAGTATCTTCCTT113121.3767084109192256No Hit
AGTGGAACTGTTATTATCCCATCAAATGGCACAGCTGAGACCAAGTCTCT102601.248676475957501No Hit
TGGGGAAAAATATGCTTTGAGCTACAGAAAAACATAGAAATCAGAGACCA92591.1268514123674955No Hit
GACTACAGATGTGCCACCATACCAACTGCTCTGGAACATGTTAGACCAAG88041.0714763834629473No Hit
TCAAAGGGCTCCACTGGTTTTTCTTATCTCTTTCATTTGATTAGACCAAG84911.0333832317110274No Hit
GAAAGTGGTGCATTGATGGAAGGCCTGATGTAGGTCTCAGACCAAGTCTC78150.9511117601956991No Hit
GCCAAACGAAAATTATGGCAGGTTGTTACGAGGCATTGGATGATTCAGAG74300.9042559665072354No Hit
GAAAGTGGTGCATTGATGAAGGCCTGATGTAGGTCTCAGACCAAGTCTCT73710.8970754682536785No Hit
TGTAGTTTCTAAGGGTCGAATTCTTTAAGTTCACTGGTATTTGAGACCAA70950.8634853408302603No Hit
GGGCAGTTGGCCACTTTTACTTATGGCTGGGTGCAGAGACCAAGTCTCTG68760.8368323049399394No Hit
AGTTCCATTTAGGTTGCTGGTGGTGAAGAACATTCAGGCAAGACCAAGTC68400.8324509839716675No Hit
TTACCCAGTACCATCAATGCTGTTAATGAGAGAAGTTTCTGAGAGGTAGA67380.8200372412282303No Hit
CTGAGGGAAGCAGCAATTCTCTGCTACCGTACCAAGAGGGGGAAGAGGGA64800.7886377742889481No Hit
TTCTGATGAGCCAAGTCTCTGTGGGAGGAGGGCAGTACACAGACCAAGTC63100.7679482030498862No Hit
GGGCAGTTGGCCACTTTACTTATGGCTGGGTGCAGAGACCAAGTCTCTGC62370.7590638577531126No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA61690.7507880292574878No Hit
GCTGCCCCAAAGTGTATAGAAGCCAAAATGATAAGAAACTAAGAGACCAA60090.7313154916207236No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTAAAG59420.7231613664853287No Hit
CCCAGTTGGTACTGGAAATCAACTAGTGACCTTCCAGGGACAACCCGAAC59100.7192668589579758No Hit
TCTATTGTGATGGCCACGTTACACTGACGACATGGTAAAGAGACTTCAGT58720.7146421312692444No Hit
TCCCTGCCAGCGAGATACACTGACGACATGGTTCTACATTCCTGCCATCA58510.7120863607044191No Hit
GGCTCCATAGGAAAGTAACAGCAACAGAGGAGGGGGAGACCAAGTCTCTG57660.7017415750848881No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC56640.689327832341451No Hit
CGCCCGGCCTTCATTTTCTTAAATAACTATTTTGTTGTTTTTACTTTTTA56220.6842162912118003No Hit
TTGTCCTGTATATTGGTTCTGTCCTTTATGGATACATTAAGAACTTTTTG56040.6820256307276643No Hit
GCTGCCCCAAAGTGTAAAGAAGCCAAAATGATAAGAAACTAAGAGACCAA55130.6709506249467547No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTAAAGTACA54690.6655956770966446No Hit
GCTTTCACTTCTCTGGCTGAACACACAGCTCAGAATACTAGTAGACCAAG49130.5979286088088892No Hit
GGGGTCAGAGGCAAGCAGATATCCGGTGTAGACCAAGTCTCTGCTACCGT48340.5883140433507369No Hit
ATCCTGTTCCAAGTCAAGACCACGTCTCTGCTACCTTATGCTCAGTGCTG47720.5807684350164908No Hit
AGCACCAAACGTAAAGCAGCAACAGAGGAGGGGGAGACCAAGTCTCTGCT47560.5788211812528145No Hit
CATCTCCATGAGTAGGAAGAAGGCCTGATGTAGGTCTCAGACCAAGTCTC46250.5628780410627138No Hit
TGATTCTCCACTTCATGCTGTCAAAAAGCGCAGATTTCCTATAAACAGAG45820.5576447965728334No Hit
CAAAGTGGGAAGGGGAGTGAACATCGAATCATGGAATCCCAGACCAAGTC45680.5559409495296165No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC45570.554602212567089No Hit
TCAAAGGGCTCCACAGTTTTTCTTATCTCTTTCATTTGATTAGACCAAGT45560.5544805092068592No Hit
GTATGAGTTTTAGAGGCTGTTAATTTGCTATACACTGACGACATGGAGGA42820.5211337885039006No Hit
TCTACCCACTCTCTTTTCAGTGCTACGGTAGCAGAACTGTCAAATGACCA39990.486691737558874No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAAA39820.48462278043496776No Hit
GTTGTTTCAGAACACCGAAAGCTCAAGAAGCATGTCATAGACCAAGTCTC37860.4607689218299317No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAAAAATGAAATACTT37470.4560224907809705No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGAAAATGA36160.4400793505908698No Hit
TGGTGATAGATGACTTGCTGCTACACTGACCCATGTGGAATAGTTCCCAG35780.4354546229021383No Hit
CAAAGTGGGAAGGGGAGTGAATATCGAATCATGGAATCCCAGACCAAGTC35590.4331422590577726No Hit
GAAGTATTACAAATGGAAGATGATCTGGTGCACTTTTCTGAGGACCATAA34960.4254749473632967No Hit
TTCCAGTTCATTTATCCAAGTTGTTTTTACATTACCATCAATGTCATCAA34290.41732082222790173No Hit
AGACATCGCTGAATTTAAAGGATACAGATGAAATTGCTGCCACAGACCAA34050.4143999415823871No Hit
AGTCTTCTCCTAAACCATAGTCTGCTACCGTATACAGCAGAAGAACGTGC33730.41050543405503426No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTAAAGT33620.40916669709250675No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCAAGGTAATTAAA33550.4083147735708983No Hit
AAAAGCAGTGGTCCAATGTATCTGCTACCGTAGTGCAGATAATGACAAGG33520.40794966349020895No Hit
GTTGTTTCAGAACACCGAAAAGCTCAAGAAGCATGTCATAGACCAAGTCT33150.40344663916170725No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG32820.39943042827412467No Hit
CATCTCCATGAGTAGGATGAAGGCCTGATGTAGGTCTCAGACCAAGTCTC31420.382391957841956No Hit
CTACTAATGCCCACAACGAGATACTTTCCTGAGTGCCAGACCAAGTCTCT31150.37910596711575206No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA28860.3512358976231334No Hit
AGTTCCATTTAGGTTGCTGGTGAAGAACATTCAGGCAAGACCAAGTCTCT28730.3496537539401463No Hit
GGTGCCAGTTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACCG28440.3461243564934828No Hit
TCTCCCCCACCCCCTCTACTCACTCAAAGACATCTGCAGACCAAGTCTCT27880.3393089683206153No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC27720.3373617145569389No Hit
CTACTAATGCCCACAAAGAGATACTTTCCTGAGTGCCAGACCAAGTCTCT27620.3361446809546411No Hit
TGGCTTATAAAATATTAATGTGCTTCTGTTTCAAAAGACCAAGTCTCTGC27480.33444083391142426No Hit
CCAAAATATGTCTGGATTGGAGAATACGGTAGCACAGCAAGTGGAAAATC27190.3309114364647608No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTAAAGTAC26650.3243394550123529No Hit
AGTCTTCTCCTAAACCATAGTCTGCTACCGTACTATATCCCAGGCTTTGC26290.31995813404408097No Hit
TGTCATAATAAGAACAATGAAAGTTGTACACTGACACAATGGCAACAGTT25600.3115606021882264No Hit
TTTTAAGATGGTGTTTAAAAGAAGTAACCCAGAACTGTCAAATGACCAAG25530.310708678666618No Hit
CTCCTTTCTGGACGCTTTTGCTACCGTATGGAGAGAAATCTGTATTAACA24730.3009724098482359No Hit
CACTTAATGAGACCAACAGTAACACCACTGACTCTGATCCAGAAGACCAA23200.2823517957330802No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACAAGGTAATTAAATGTGGTAC22720.27651003444205097No Hit
GCTTAGATTCCTCTTTGGAGTCAGTGTACCTCTCACAATATTAAATGCAC22550.2744410773181447No Hit
AAAAGCAGTGGTCCAATGTATCTGCTACCGTACCAGTCAAATGTCCATGG22430.27298063699538744No Hit
CTCCTCCACAGCTCGCTACCGTAGACATTCAGAGTGAAGAAATTTTACAA21510.26178392785424803No Hit
TTCCTGAGTTTTCATGGACAGCAAATGATCAGCCTTGCAAAGACCAAGTC21440.26093200433263963No Hit
GGGGTCAGAGGAAGCAGATATCCGGTGTAGACCAAGTCTCTGCTACCGTA19420.23634792556622486No Hit
TGAATGTAGCACGCATTCACATAGGTTAACATTTACCACCCCTAGACCAA19250.2342789684423187No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTAAAGTACACTCATC19220.23391385836162934No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTAAAGT19170.23330534156048047No Hit
TCTCCCCCACCCCTTCTACTCACTCAAAGACATCTGCAGACCAAGTCTCT18150.22089159881704337No Hit
CAGCAATGTGGCTTTAAGTAGAACATATCATCTGGACCCTTTTCAGACCA17950.21845753161244785No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC17450.212372363600959No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTAAAATG17040.2073825258315382No Hit
CCAAATCTGCTTTCTTGATAAAATCCTCTGAATGGCATGCAGACCAAGTC16960.2064088989497No Hit
TGCATTCTGAAAAAGCCAGAATTTACAGCTCTCCAGACCAAGTCTCTGCT16320.19861988389499435No Hit
TAGATTGATGACCAAAAGAACTGCACACTGACGACATGGTTCTACATTCC16280.19813307045407524No Hit
GCTTAGATTCCTCTTTGGAGTCAGTGCACAGCTGCAAAAACACTTGTTCA15990.19460367300741174No Hit
AAGTCCTCCATTTCTGTATCCATGGATTCTGAATGTGCTTAGACCAAGTC15680.1908308688402887No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAA15560.18937042851753136No Hit
AGAAGACCAGGAAAGAACGGGAGCTTCTTATGGCCAAGAGACCAAGTCTC15430.18778828483454427No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGAAAATGAAATACTTTGA15190.18486740418902967No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTAAAGTA14250.17342728832743073No Hit
TGTAGTTTCTAAGGGACAAATTCTTTAAGTTCACTGGTATTTGAGACCAA14230.17318388160697118No Hit
GTGCTGGTAAATTCACTCCATCGAATCATCACCAATAGACCAAGTCTCTG13900.16916767071938857No Hit
GCTTTCACTTCTCTGGCTGAGCACACAGCTCAGAATACTAGTAGACCAAG13800.16795063711709082No Hit
GCCTGCTGAAAATGCCAGAACCCCTCAGATGTTAGACCAAGTCTCTGCTA13740.16722041695571213No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA13480.16405612958973798No Hit
GCCACACCTCACGTTAGAATTTTCCAGTGAAGACCAAGTCTCTGCTACCG13350.16247398590675088No Hit
TGGTGATAGATGACTTGCTGCTACCGTAAGCCTGCAGTGATATTAACTGT12570.15298112380882836No Hit
CACTTAATGAGACCAACAGTAACACCTTTATCACTCACAAGACCAAGTCT12150.14786958267917777No Hit
GGCTTCCACTTAATTCCAGTCTAAAAAGTTACCCAGAAGTGTAGACCAAG12150.14786958267917777No Hit
TCGGGGGCCTACAAGAGTCTGACAGAGTTCTCATTACCCAAGACCAAGTC11340.1380116105005659No Hit
GCCTGCTGAAAATGACTGAACCCCTCAGATGTTAGACCAAGTCTCTGCTA11120.13533413657551085No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTAACCCTCAATTTGGAAGCCTC11020.1341171029732131No Hit
TGGGTCCAGTCAGTGAACGAGATACTTTCCTGAGTGCCAGACCAAGTCTC10300.12535446103666922No Hit
AGTTGCTTAGCCTTTGCAATACCAATTTCTTTCATGCCAAGTTTAGACCA10050.12231187703092483No Hit
GCGTCTTCACCTGGAAGGCAGTCTGGCTGAGACCAAGTCTCTGCTACCGT10050.12231187703092483No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG9880.12024291990701864No Hit
CTCCTCCACAGCTCGCTACACTGACGACATTCAGAGTGAAGAAATTTTAC9250.11257560821254274No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG8780.10685555028174328No Hit
AACTGATCACCTTGAATCTGCTTGGGAGAAGGAATATTTGATGGTCAACC8490.10332615283507977No Hit
GTTTTTGTACAGAGAATAGTTGTAGTTGACACTGACGACATGGTGGTTGA8470.10308274611462022No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT8280.10077038227025448No Hit
GATGTGCCACCATACCCAGAGACCAAGTCTCTGCTACCGTAAAGTACACT8230.1001618654691056No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)