FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100604

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100604
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences821670
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG192132.338286660094685No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT177822.164129151605876No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA175252.132851388026823No Hit
ATGTGTCCCAGCTAAATGTGGTGTCCTTTGCATTCAGTATGTAGAACCAT161901.9703774021200726No Hit
GCATGCCATTCAAAGGATTTTATCAAGAAAGCAGATTTGGTGTAGAACCA159791.94469799311159No Hit
CGACTCAGAGACCGCTTGCTGGGAATTGTAGTTAATGTAGAACCATGTCG159501.9411685956649265No Hit
CAGAACTGTTGCCATTGTGTCAGTGTCCAAGAAACTTTTAGCACCAATGT159121.9365438679761946No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT157281.9141504496939161No Hit
AATTCTGCTGAATGGGTTGCTGGGTTGTGTCAAATATATAAGTTCATGTA156701.9070916548005892No Hit
TAAGGAGAATGATTTGTAGTGGCCAAAATTGAATGCTATGCTTAGATGTA149521.8197086421556101No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT146441.7822240072048388No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC141551.7227110640524785No Hit
TTGCAAGGCTGATCATTTGCTGGTGTGTAACGTTCCTCTTGTAGAACCAT132251.609526939038787No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTACAAATAGACGAAA127571.5525697664512517No Hit
TCTGGATCAGAGTCAGTGGTGTGTTACTGTTGGTCTCATTAAGTGTGTAG127161.5475799286818308No Hit
ATGATCCATGGACATTTGACTGGTAGGTAAGTGGAGTGAAATTTAATGTA123771.5063224895639369No Hit
CAGCTGTGCCATTTGAGGGATAATAACAGTTCCACTTGTAGAACCATGTC122891.4956125938637166No Hit
GGTGAAATAAAGGAAGATACTAGTTTTGGACTTGGTCTAGTGAGGATGAA111251.3539498825562575No Hit
CTGATTTCTATGTTTTTCTGTAGATCAAAGCATATTTTTCCCCATGTAGA111121.3523677388732704No Hit
CAGCTGTGCCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGT101681.2374797668163617No Hit
CTGATTTCTATGTTTTTCTGTAGCTCAAAGCATATTTTTCCCCATGTAGA91141.109204425134178No Hit
AACATGTTCCAGAGCAGTTGGTATGGTGGCACATCTGTAGTCTGTAGAAC87251.061861818004795No Hit
AATCAAATGAAAGAGATAAGAAAAACCAGTGGAGCCCTTTGATGTAGAAC83551.016831574719778No Hit
GAGACCTACATCAGGCCTTCCATCAATGCACCACTTTCTGTAGAACCATG77060.9378460939306533No Hit
GAGACCTACATCAGGCCTTCATCAATGCACCACTTTCTGTAGAACCATGT72790.8858787591125392No Hit
GTTGTAAAATTTCTTCACTCTGAATGTCAGCAAAAACCTTATGTGAATGC72330.8802804045419693No Hit
CAAATACCAGTGAACTTAAAGAATTCGACCCTTAGAAACTACATGTAGAA70210.8544792921732569No Hit
CTGCACCCAGCCATAAGTAAAAGTGGCCAACTGCCCTGTAGAACCATGTC67870.8260007058794894No Hit
TGCCTGAATGTTCTTCACCACCAGCAACCTAAATGGAACTTGTAGAACCA67200.8178465807440943No Hit
ACCTCTCAGAAACTTCTCTCATTAACAGCATTGATGGTACTGGGTAATGT66710.8118831160928354No Hit
TCCCTCTTCCCCCTCTTGGTACGGTAGCAGAGAATTGCTGCTTCCCTCAG63450.7722078206579284No Hit
GTGTACTGCCCTCCTCCCACAGAGACTTGGCTCATCAGAATGTAGAACCA62360.7589421543928828No Hit
CTGCACCCAGCCATAAGTAAAGTGGCCAACTGCCCTGTAGAACCATGTCG61650.7503012158165687No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTATCTCG61070.7432424209232417No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT60800.7399564301970377No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA59150.7198753757591247No Hit
CTTAGTTTCTTATCATTTTGGCTTCTATACACTTTGGGGCAGCTGTAGAA58870.716467681672691No Hit
GGAAGTTTAACTGAAGTCTCTTTACCATGTCGTCAGTGTAACGTGGCCAT57960.7053926758917813No Hit
CCCCCTCCTCTGTTGCTGTTACTTTCCTATGGAGCCTGTAGAACCATGTC57000.6937091533097229No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG55720.6781311232003115No Hit
TGGCCATAGGTTCCTTCTCCAATAATCCCAATAATGTCAAACTTGTCCAC54990.6692467779035379No Hit
CTTAGTTTCTTATCATTTTGGCTTCTTTACACTTTGGGGCAGCTGTAGAA54300.6608492460476834No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC54050.6578066620419389No Hit
ACCAAATTTTCCCTGGGTTTACCTTTTTCTTTAGGACTTTCTAAATGGGT52410.6378473109642557No Hit
CACTAGTACCTTGCTCTTTTTCATCAAAAAGGTTTTTCACTTTGTCCAAA52340.6369953874426473No Hit
ACTAGTATTCTGAGCTGTGTGTTCAGCCAGAGAAGTGAAAGCTGTAGAAC48630.5918434407974004No Hit
ACACCGGATATCTGCTTGCCTCTGACCCCTGTAGAACCATGTCGTCAGTG48160.5861233828666009No Hit
CCCCCTCCTCTGTTGCTGCTTTACGTTTGGTGCTTGTAGAACCATGTCGT46520.5661640317889177No Hit
GAGACCTACATCAGGCCTTCTTCCTACTCATGGAGATGTGTAGAACCATG45570.554602212567089No Hit
AATCAAATGAAAGAGATAAGAAAAACTGTGGAGCCCTTTGATGTAGAACC45150.5494906714374385No Hit
TCCAATCACATACAATAGAATATCAACTTTTCTACTAAGGATTAAAAATA44660.5435272067861794No Hit
GGGATTCCATGATTCGATGTTCACTCCCCTTCCCACTTTGTGTAGAACCA44620.5430403933452603No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG43320.5272189565153894No Hit
CAGCTGCCTACTTCCTCCATGTCGTCAGTGTATAGCAAATTAACAGCCTC42970.5229593389073472No Hit
TGGTCATTTGACAGTTCTGCTACCGTAGCACTGAAAAGAGAGTGGGTAGA39370.47914612922462785No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG39270.47792909562233016No Hit
ATGACATGCTTCTTGAGCTTTCGGTGTTCTGAAACAACTGTAGAACCATG37400.455170567259362No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTTGTCTAAGAACACAGAGGAGAAT36840.44835517908649464No Hit
ATGGGCAGCACTGAGCATAAGGTAGCAGAGACGTGGTCTTGACTTGGAAC36710.44677303540350755No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTCTTGGTCTGTCTAAGAACACAGA35940.4374018766658147No Hit
GGGATTCCATGATTCGATATTCACTCCCCTTCCCACTTTGTGTAGAACCA35300.42961286161110906No Hit
GGGAACTATTCCACATGGGTCAGTGTAGCAGCAAGTCATCTATCACCATG35180.42815242128835174No Hit
TATGGTCCTCAGAAAAGTGCACCAGATCATCTTCCATTTGTAATACTTCT34480.41963318607226746No Hit
GTGGCAGCAATTTCATCTGTATCCTTTAAATTCAGCGATGTCTTGTAGAA33740.410627137415264No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT33440.40697603660837073No Hit
AAGAGCACGTTCTTCTGCTGTATACGGTAGCAGACTATGGTTTAGGAGAA33420.4067326298879112No Hit
ATGACATGCTTCTTGAGCTTTTCGGTGTTCTGAAACAACTGTAGAACCAT32500.3955359207467718No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG32360.39383207370355494No Hit
TGCACGTACCACATTTAATTACCTTGAGACTTGGTCTTCTCTTGGTATTA32320.39334526026263583No Hit
ACTAGATATTCCTTGTCATTATCTGCACTACGGTAGCAGATACATTGGAC32260.3926150401012572No Hit
GATTGGCTAACAATTCAGATTGCATTTTAGCTAAAGACCTTTTTGAAATT31860.38774690569206616No Hit
GAGACCTACATCAGGCCTTCATCCTACTCATGGAGATGTGTAGAACCATG31110.37861915367483295No Hit
GGCACTCAGGAAAGTATCTCGTTGTGGGCATTAGTAGTGTAGAACCATGT30780.37460294278725037No Hit
GCAGATGTCTTTGAGTGAGTAGAGGGGGTGGGGGAGATGTAGAACCATGT30020.3653534874097874No Hit
GAGTCCTCCTTCTGTGAGCAAACTGGCACCTGTAGAACCATGTCGTCAGT28730.3496537539401463No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT28710.3494103472196867No Hit
TGCCTGAATGTTCTTCACCAGCAACCTAAATGGAACTTGTAGAACCATGT28190.3430817724877384No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG27690.33699660447624963No Hit
GGCACTCAGGAAAGTATCTCTTTGTGGGCATTAGTAGTGTAGAACCATGT27170.3306680297443012No Hit
TTTGAAACAGAAGCACATTAATATTTTATAAGCCATGTAGAACCATGTCG27040.3290858860613141No Hit
ACAGATTTTCCACTTGCTGTGCTACCGTATTCTCCAATCCAGACATATTT26630.3240960482918933No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT26530.32287901468959557No Hit
TGTGCAAAGCCTGGGATATAGTACGGTAGCAGACTATGGTTTAGGAGAAG25400.3091265349836309No Hit
CAGAACTGTTGCCATTGTGTCAGTGTACAACTTTCATTGTTCTTATTATG25170.30632735769834607No Hit
TGGTCATTTGACAGTTCTGGGTTACTTCTTTTAAACACCATCTTAAAATG25080.3052320274562781No Hit
CAGACTGTTAATACAGATTTCTCTCCATACGGTAGCAAAAGCGTCCAGAA24390.2968344956004235No Hit
TCTGGATCAGAGTCAGTGGTGTTACTGTTGGTCTCATTAAGTGTGTAGAA22600.27504959411929364No Hit
GGAGAGCTGTAAATTCTGGCTTTTTCAGAATGCATGTAGAACCATGTCGT22230.2705465697907919No Hit
TGAAGTGCATTTAATATTGTGAGAGGTACACTGACTCCAAAGAGGAATCT22120.2692078328282644No Hit
TGCACGTACCACATTTAATTACCTTGTCTTCTCTTGGTATTAGTGCTTGA22020.26799079922596664No Hit
ATGATCCATGGACATTTGACTGGTACGGTAGCAGATACATTGGACCACTG21940.2670171723441284No Hit
GTTGTAAAATTTCTTCACTCTGAATGTCTACGGTAGCGAGCTGTGGAGGA21070.2564289800041379No Hit
TTGCAAGGCTGATCATTTGCTGTCCATGAAAACTCAGGAATGTAGAACCA20980.2553336497620699No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT19860.24170287341633503No Hit
ACACCGGATATCTGCTTCCTCTGACCCCTGTAGAACCATGTCGTCAGTGT19160.23318363820025073No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT18970.23087127435588495No Hit
AGGGGTGGTAAATGTTAACCTATGTGAATGCGTGCTACATTCATGTAGAA18780.22855891051151925No Hit
GCAGATGTCTTTGAGTGAGTAGAAGGGGTGGGGGAGATGTAGAACCATGT18010.21918775177382646No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTGCAGTT18000.2190660484135967No Hit
GAAAAGGGTCCAGATGATATGTTCTACTTAAAGCCACATTGCTGTGTAGA17740.21590176104762251No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG17050.20750422919176797No Hit
GCATGCCATTCAGAGGATTTTATCAAGAAAGCAGATTTGGTGTAGAACCA16870.205313568707632No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTACTTGGTCTGTCTAAGAACACAG16780.20421823846556403No Hit
GAACAAGTGTTTTTGCAGCTGTGCACTGACTCCAAAGAGGAATCTAAGCT15750.19168279236189711No Hit
AAGCACATTCAGAATCCATGGATACAGAAATGGAGGACTTTGTAGAACCA15360.18693636131293584No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA15240.18547592099017854No Hit
CTTGGCCATAAGAAGCTCCCGTTCTTTCCTGGTCTTCTTGTAGAACCATG14950.18194652354351504No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTCTAAGAACACAGAGGAGAATTTA14940.18182482018328527No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC14260.1735489916876605No Hit
CAAATACCAGTGAACTTAAAGAATTTGTCCCTTAGAAACTACATGTAGAA14040.17087151776260542No Hit
ATTGGTGATGATTCGATGGAGTGAATTTACCAGCACTGTAGAACCATGTC13690.16661190015456329No Hit
ACTAGTATTCTGAGCTGTGTGCTCAGCCAGAGAAGTGAAAGCTGTAGAAC13650.16612508671364415No Hit
AACATCTGAGGGGTTCTGGCATTTTCAGCAGGCTGTAGAACCATGTCGTC13570.16515145983180596No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT13330.16223057918629133No Hit
TCACTGGAAAATTCTAACGTGAGGTGTGGCTGTAGAACCATGTCGTCAGT13120.15967480862146605No Hit
AGACAGTTAATATCACTGCAGGCTTACGGTAGCAGCAAGTCATCTATCAC12280.14945172636216486No Hit
ACACTTCTGGGTAACTTTTTAGACTGGAATTAAGTGGAAGCCTGTAGAAC11940.1453138121143525No Hit
TGTGAGTGATAAAGGTGTTACTGTTGGTCTCATTAAGTGTGTAGAACCAT11820.14385337179159516No Hit
TGGGTAATGAGAACTCTGTCAGACTCTTGTAGGCCCCCGATGTAGAACCA11090.13496902649482154No Hit
AACATCTGAGGGGTTCAGTCATTTTCAGCAGGCTGTAGAACCATGTCGTC10900.13265666265045578No Hit
AAGAGGCTTCCAAATTGAGGGTTACGGTAGCAGAGACCTGTGGGAAGCGA10820.1316830357686176No Hit
GGCACTCAGGAAAGTATCTCGTTCACTGACTGGACCCATGTAGAACCATG10260.12486764759575013No Hit
AAACTTGGCATGAAAGAAATTGGTATTGCAAAGGCTAAGCAACTTGTAGA9930.12085143670816752No Hit
CAGCCAGACTGCCTTCCAGGTGAAGACGCTGTAGAACCATGTCGTCAGTG9900.12048632662747817No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT9770.11890418294449111No Hit
TTGGGCAGCACTGAGCATAAGGTAGCAGAGACGTGGTCTTGACTTGGAAC9630.11720033590127422No Hit
GTTGTAAAATTTCTTCACTCTGAATGTCGTCAGTGTAGCGAGCTGTGGAG9010.10965472756702813No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT8690.10576022003967529No Hit
GGTTGACCATCAAATATTCCTTCTCCCAAGCAGATTCAAGGTGATCAGTT8540.10393466963622866No Hit
CTGGGTATGGTGGCACATCTGTAGAACCATGTCGTCAGTGTAGATCTCGG8360.10174400915209268No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT8260.10052697554979494No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)