FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18759
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACGCGTATCATAT507927.07500399808092No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA287815.341969188123034No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTACGCGTATCATATCTCG9294.952289567674184No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTACGCGTATC5713.0438722746415054No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA3701.9723865877712032No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTACGCGTATCATATCT2671.4233168079321925No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACGCGTATCATAT2361.258062796524335No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA2141.1407857561703716No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACGCGTATCATAC1921.023508715816408No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAC1690.9009009009009009No Hit
GCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTACGCG1440.7676315368623061No Hit
GGGGTCAGGAGGAGACCAAGTCTCTGCTACCGTACGCGTATCATATCTCG1250.6663468201929741No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTACGC1220.6503544965083426No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACGCGTATCATAA1190.6343621728237113No Hit
GGAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACGCGTATCATAT1170.6237006237006237No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACGCGTATCATAG1060.565062103523642No Hit
GGTCTTGGCCAGTTGGAGACCAAGTCTCTGCTACCGTACGCGTATCATAT980.5224159070312917No Hit
TCAACAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTACGCG970.5170851324697479No Hit
TCCACAAACAGAACAAGATGCTAGACCAAGTCTCTGCTACCGTACGCGTA880.4691081614158537No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC850.4531158377312223No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG850.4531158377312223No Hit
GCAACAGAGGAGGGGAAGACCAAGTCTCTGCTACCGTACGCGTATCATAT840.44778506316967853No Hit
GCGTCTTCACCTGGACTATGTCCGGAGACCAAGTCTCTGCTACCGTACGC840.44778506316967853No Hit
TCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA760.4051388666773282No Hit
GCAACAGAGGAGGAGGAGACCAAGTCTCTGCTACCGTACGCGTATCATAT690.3678234447465217No Hit
GCAACAGAGGACCAGGAGACCAAGTCTCTGCTACCGTACGCGTATCATAT650.3465003465003465No Hit
GGGGCCAGTTGGAGACCAAGTCTCTGCTACCGTACGCGTATCATATCTCG640.3411695719388027No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC600.31984647369262753No Hit
GCAACAGGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTACGCGTATCAT550.29319260088490856No Hit
TGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTACGCGTATCATATCTCG500.2665387280771896No Hit
AGATGAGTCATATTTGTGGGTTTTCATAGACCAAGTCTCTGCTACCGTAC470.25054640439255826No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTACGC460.24521562983101444No Hit
ACAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACGCGTATCATAT430.2292233061463831No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC420.22389253158483927No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTACGCGTATCATACCTCG410.2185617570232955No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT390.2079002079002079No Hit
GGGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTACG360.19190788421557653No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG360.19190788421557653No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTACGCGTATCATAACTCG360.19190788421557653No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC360.19190788421557653No Hit
GCAACAGATGAGGGGGAGACCAAGTCTCTGCTACCGTACGCGTATCATAT350.18657710965403274No Hit
TACCCTCCATGAGGCATAACTGCACCCTTAGACCAAGTCTCTGCTACCGT320.17058478596940135No Hit
AGCTGCTCACCATCGCTACACCCAGGTCTTGGCCAGTTGGAGACCAAGTC320.17058478596940135No Hit
ATACGGCCAGGAGGAGACCAAGTCTCTGCTACCGTACGCGTATCATATCT320.17058478596940135No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATAGTGGGGGCAGCGCCT310.16525401140785756No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT300.15992323684631377No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA290.15459246228476997No Hit
TTTCGCTTCCCACAGGTCTCTGCTACGGAAGGCAGTCTGGCTGAGACCAA270.14393091316168238No Hit
GTAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACGCGTATCATAT270.14393091316168238No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTAGACCAAG260.1386001386001386No Hit
GGACCAGAGGAAACAGACCAAGTCTCTGCTACCGTACGCGTATCATATCT260.1386001386001386No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTAGAC250.1332693640385948No Hit
GCAACAGAGGAGGGGAGACCAAGTCTCTGCTACCGTACGCGTATCATATC240.127938589477051No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC240.127938589477051No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA230.12260781491550722No Hit
GCGACAGGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTACGCGTATCAT220.11727704035396343No Hit
TAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTACGCGTATC210.11194626579241963No Hit
ACAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA210.11194626579241963No Hit
CGGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTACG210.11194626579241963No Hit
GCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTACGCGTATCATA200.10661549123087584No Hit
GTAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA200.10661549123087584No Hit
GGAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA200.10661549123087584No Hit
GCAACAGAGGAGGTGGCTTAGGTCAGCAGACCAAGTCTCTGCTACCGTAC190.10128471666933204No Hit
GCACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACGCGTATCATATC190.10128471666933204No Hit
CAGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTACG190.10128471666933204No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)