FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100612

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100612
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18759
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG549229.276613891998508No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT277014.766245535476305No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC10275.474705474705475Illumina Single End PCR Primer 1 (100% over 21bp)
TGCCCTGACTTTCAACTCTGTGTAGAACCATGTCGTCAGTGTAGATCTCG5622.995895303587611No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT3491.8604403219787835No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC2761.4712937789860867No Hit
CCCCCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG2511.3380244149474918No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA1630.8689162535316381No Hit
CCATGGGACTGACTTTCTGCTCTTTTCACCCATCTACAGTCCCCCTTGCC1490.7942854096700251No Hit
TGCCCTGACTTTCAACTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGAT1390.7409776640545871No Hit
CCTCCTGACCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC1350.7196545658084119Illumina Single End PCR Primer 1 (95% over 22bp)
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA1250.6663468201929741No Hit
CCCCCTCCTCTGTTCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG1210.6450237219467989No Hit
CCAACTGGCCAAGACCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG1160.6183698491390799No Hit
CCTCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG1050.5597313289620982No Hit
TCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG990.5277466815928354No Hit
TGCCCTGACTTTCAACTCTGTTGATGTAGAACCATGTCGTCAGTGTAGAT940.5010928087851165No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC940.5010928087851165No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC830.44245428860813474No Hit
AAGACCCAGGTCCAGATGATTCTTTAACAGGGCAGCTACGGTTTCCTGTA830.44245428860813474No Hit
CCGGACATAGTCCAGGTGAAGACGCTGTAGAACCATGTCGTCAGTGTAGA830.44245428860813474No Hit
AGCATCTTGTTCTGTTTGTGGATGTAGAACCATGTCGTCAGTGTAGATCT800.42646196492350336No Hit
CCAACTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC730.38914654299269685Illumina Single End PCR Primer 1 (95% over 22bp)
CCTGGTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG720.38381576843115306No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGT690.3678234447465217No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC590.31451569913108374No Hit
CCTCCTGGCCCATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC590.31451569913108374Illumina Single End PCR Primer 1 (95% over 22bp)
CCTCCTGGCCCCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGT550.29319260088490856No Hit
CCTGGTCCTCTGACTGCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGA480.255877178954102No Hit
CCCCCTCCTCTGTTGTTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG470.25054640439255826No Hit
ATGAAAACCCACAAATATGACTCATCTTGTAGAACCATGTCGTCAGTGTA470.25054640439255826No Hit
CCCCCTCATCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG420.22389253158483927No Hit
CTTGGGCCTGTGTTATCTCCCCTCCCTGTAGAACCATGTCGTCAGTGTAG380.2025694333386641No Hit
CCTCCTGGCCGTATTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC360.19190788421557653No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG360.19190788421557653No Hit
CCAACTGGCCAAGACCTGGGTGTAGCGATGGTGAGCAGCTTGTAGAACCA320.17058478596940135No Hit
AAGGGTGCAGTTATGCCTCATGGAGGGTATGTAGAACCATGTCGTCAGTG300.15992323684631377No Hit
GTTTCCTCTGGTCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC290.15459246228476997No Hit
CCCCCTCCTCTGTTACTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG280.14926168772322618No Hit
CTCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG280.14926168772322618No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA280.14926168772322618No Hit
CCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT270.14393091316168238No Hit
CCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG260.1386001386001386No Hit
CAGCCAGACTGCCTTCCGTAGCAGAGACCTGTGGGAAGCGAAATGTAGAA260.1386001386001386No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA250.1332693640385948No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGTGTAGAAC240.127938589477051No Hit
TGCCCTGACTTTCAACTCTATGTAGAACCATGTCGTCAGTGTAGATCTCG230.12260781491550722No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGTA230.12260781491550722No Hit
TCTAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG220.11727704035396343No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC220.11727704035396343No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA210.11194626579241963No Hit
CCTCCTGGCCCCTGTCGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGT210.11194626579241963No Hit
CTTGGGCCTGTGTTATCTCCCCTCCGTGTAGAACCATGTCGTCAGTGTAG210.11194626579241963No Hit
CCCCCTCCTCTGTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT210.11194626579241963No Hit
GCTGACCTAAAGCCACCTCCCCTGTTGCTGTAGAACCATGTCGTCAGTGT210.11194626579241963No Hit
GCTGACCTAAAGCCACCTCCTCTGTTCCTGTAGAACCATGTCGTCAGTGT200.10661549123087584No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG200.10661549123087584No Hit
CCTGGTCCTCTGACTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTG200.10661549123087584No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT190.10128471666933204No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC190.10128471666933204No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGTTGTAGAACCATGTCGTCAGTGT190.10128471666933204No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG190.10128471666933204No Hit
CTTGGGCCTGTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG190.10128471666933204No Hit
TCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC190.10128471666933204Illumina Single End PCR Primer 1 (100% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)