FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174220
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC71444.100562507174836No Hit
ATGAAAACACAACATGAATATAAACATCAATATTTGAAATAGAAAATCAA69944.014464470210079No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC50972.9256112960624496No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG49182.8228676386178395No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC43222.480771438411204No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA38432.20583170703708No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA37572.1564688325106185No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG35492.0370795545861555No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC35202.0204339341063022No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC29111.6708759040293883No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA27541.5807599586729424No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT27271.5652623120192861No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG27201.5612444036275974No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC25171.444725060268626No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT24301.3947881988290667No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC23951.3746986568706234No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG23891.371254735392033No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT23081.3247617954310642No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT23011.3207438870393755No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC22751.3058202272988175No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT21661.2432556537710941No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG20971.2036505567673057No Hit
GGCCTTTTCCTTCAAACAGGATTATTTACCCTTATAATGTCTCACCAATG20071.1519917345884514No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA20041.1502697738491563No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT19621.1261623234990243No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA18381.0549879462748248No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT18091.0383423257949718No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT17871.0257146137068076No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT17861.025140626793709No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA17561.0079210194007577No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCATT17030.9774997130065435No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTT15930.9143611525657215No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA15700.9011594535644587No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT15520.8908276891286879No Hit
TCCAGGAAGAGGAAAGGAAAAACATCAAAAAATAACTTACCTTTTTGTCT15280.8770520032143267No Hit
ACAAGTCAACAACCCCCACAAAATGTTTAATTTAACTGACCTTAAAATTT14180.8139134427735049No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT13980.8024337045115371No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG13400.769142463551831No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC13160.7553667776374698No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT11670.6698427275858111No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCC11270.6468832510618758No Hit
TCTAGATATGGTTAAGAAAACTGTTCCAATACATGGAAGGATGAGAATTT10960.629089656755826No Hit
CAGCCAGACTGCCTTCCGGGTCACTGCCATGGAGGAGCCGCAGTCAGATC10490.602112271840202No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC10140.5820227298817587Illumina Single End PCR Primer 1 (100% over 21bp)
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT9600.5510274365744461No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA7810.44828377912983586No Hit
CCATGGGACTGACTTTCTGCTCTTTTCACCCATCTACAGTCCCCCTTGCC7640.4385260016071634No Hit
TCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTCT7350.42188038112731024No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGGTCCATAGAAAATCTTT7260.41671449890942486No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTCGTGTGGAGTATTTGGATGACA7130.4092526690391459No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGGGTG6200.3558718861209964No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGAC6070.34841005625071747No Hit
CCATGGGACTGACTTTCTGCTCTTGTCTTTCAGACTTCCTGAAAACAACG5430.3116748938124211No Hit
TGCCCTGACTTTCAACTCTGTGTAGAACCATGTCGTCAGTGTAGATCTCG4690.26919986224314085No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG3860.2215589484559752No Hit
CTGAAAACACAACATGAATATAAACATCAATATTTGAAATAGAAAATCAA3700.21237515784640112No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC2910.16703019171162897No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA2800.16071633566754678No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC2790.1601423487544484No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAATTCAGACTTTTGTAATT2600.14923659740557915No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA2380.13660888531741475No Hit
CCCCCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG2000.11479738261967627No Hit
CCCCCTCCTCTGTTGCTGTAGATCCGTGGGCGTGAGCGCTTCGAGATGTT1960.11250143496728275No Hit
CCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG1950.11192744805418436No Hit
TCTAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG1890.10848352657559408No Hit
CGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG1790.10274365744461027No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAAATTCAGACTTTTGTAA1780.1021696705315119No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)