FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173169
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT69304.0018710046255395No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG68183.9371943015204804No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA61943.57685266993515No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT52633.0392275753743454No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATGATACTCTGAT50012.8879302877535817No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT41502.3965028382678195No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC40842.358389781080909No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA36492.107190085985367No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC33311.9235544468120738No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG32591.8819765662445358No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC31351.8103702163782203No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT30241.7462709838365988No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG29651.7122002205937552No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT29061.6781294573509113No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC28111.6232697538242988No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT27281.5753396970589424No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA26601.5360716987451566No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA26451.5274096402935862No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA25571.4765922307110395No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT25161.4529159376100802No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC23501.3570558240793675No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA23101.3339570015418463No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG22911.322985060836524No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA22421.2946890032280605No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT22111.2767874157614816No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT21491.2409842408283238No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG21131.2201953005445547No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG19951.1520537740588672No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG19291.1139407168719575No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA18561.0717853657409813No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA18381.061390895599097No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG18021.0406019553153278No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC17471.0088410743262362No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC16660.9620659586877559No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA15420.8904596088214404No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA15150.8748679036086137No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG15000.866205845157043No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG14870.8586987278323488No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC14850.8575437867054727No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG14290.8252054351529431No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC12650.7305002627491064No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG11970.6912322644353204No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTATGATACTCTGATCTCG11700.6756405592224937No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG11380.6571615011924767No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT10380.5994144448486738No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC10050.5803579162552189No Hit
TTTCGCTTCCCACAGGTCTCTGCTAGGGGGCTGGGGTTGGGGTCGGGGTG8380.4839203321610681No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTATGATACTC5480.31645386876403975No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAT3840.22174869636020306No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACCTTTAT2850.1645791105798382No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATGATACTCTGAC2370.13686052353481282No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATGATACTCTGAT2360.1362830529713748No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTATGATACTCTGATCT2220.1281984650832424No Hit
TCAAATCATCCATTGCTTGGGCAGAAAGTCAGTCCCATGGAGACCAAGTC2100.12126881832198605No Hit
ATACGGCCAGGCATTGAAGTTTCATGGAAGCCAGCCCCTCAGGGCAACTG1850.10683205423603533No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)