FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100730

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100730
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52399
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG24574.689020782839367No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC16273.105021088188706No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA12682.4198935094181184No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA10552.0133972022366837No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT10381.980953834996851No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT9481.809194831962442No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA9021.7214068970781884No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC8581.637435828928033No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC8161.5572816275119754No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCC8121.5496478940437795No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG8071.5401057272085346No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC8011.5286551270062405No Hit
GGCCTTTTCCTTCAAACAGGATTATTTACCCTTATAATGTCTCACCAATG7901.5076623599687016No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA7821.4923948930323097No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT7791.4866695929311629No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT7541.4389587587549382No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC7371.4065153915151052No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG7151.3645298574400273No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT7101.3549876906047826No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCATT6851.307276856428558No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC6521.2442985553159411No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT6371.2156720548102062No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG6091.1622359205328345No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA5711.0897154525849728No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT5661.0801732857497282No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA5631.0744479856485811No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA5571.062997385446287No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT5391.0286455848394054No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTT5261.0038359510677684No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC5090.9713925838279356No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC5060.9656672837267887Illumina Single End PCR Primer 1 (100% over 21bp)
ATGAAAACACAACATGAATATAAACATCAATATTTGAAATAGAAAATCAA4740.904597415981221No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT4530.8645203152731923No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT4460.8511612817038492No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG4460.8511612817038492No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA4420.8435275482356533No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT4340.8282600812992613No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG4200.8015420141605756No Hit
TCCAGGAAGAGGAAAGGAAAAACATCAAAAAATAACTTACCTTTTTGTCT3930.7500143132502529No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC3920.7481058798832039No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC3080.5877974770510888No Hit
ACAAGTCAACAACCCCCACAAAATGTTTAATTTAACTGACCTTAAAATTT2930.559170976545354No Hit
CAGCCAGACTGCCTTCCGGGTCACTGCCATGGAGGAGCCGCAGTCAGATC2920.5572625431783049No Hit
TCTAGATATGGTTAAGAAAACTGTTCCAATACATGGAAGGATGAGAATTT2730.5210023092043741No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT2690.5133685757361781No Hit
CCATGGGACTGACTTTCTGCTCTTGTCTTTCAGACTTCCTGAAAACAACG2540.4847420752304433No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGAC2500.47710834176224737No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT2490.47519990839519843No Hit
TGCCCTGACTTTCAACTCTGTGTAGAACCATGTCGTCAGTGTAGATCTCG2330.4446649745224146No Hit
TCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTCT2310.44084810778831657No Hit
CCGGACATAGTCCAGGAGGCAGCCGAAGGGCATGAGCTGCGTGATGAGTT2140.4084047405484838No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA1830.3492433061699651No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA1810.3454264394358671No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG1800.3435180060688181No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA1480.28244813832325044No Hit
CCCCCTCCTCTGTTGCTGTAGATCCGTGGGCGTGAGCGCTTCGAGATGTT1320.2519132044504666No Hit
CCCCCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG1160.22137827057768278No Hit
CGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG1050.2003855035401439No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAATTCAGACTTTTGTAATT960.183209603236703No Hit
CCTCCTGGCCCCTGTCGAACCATGTCGTCAGTGTACTTCAATGCCTGGCC950.181301169869654No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC860.1641252695662131No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA850.1622168361991641No Hit
CCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG790.15076623599687017No Hit
CCGGACATAGTCCAGGTGAAGACGCTGTAGAACCATGTCGTCAGTGTAGA780.14885780262982118No Hit
CGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA770.1469493692627722No Hit
CTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT760.1450409358957232No Hit
CCTCCTGACCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC750.1431325025286742Illumina Single End PCR Primer 1 (95% over 22bp)
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAAATTCAGACTTTTGTAA660.1259566022252333No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC630.12023130212408635No Hit
CCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA630.12023130212408635No Hit
GCCTCAAAGACAATGGCTCCTCTACTGTAGAACCATGTCGTCAGTGTAGA620.11832286875703733No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA590.11259756865589039No Hit
CATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT580.11068913528884139No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGT580.11068913528884139No Hit
CTGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT560.10687226855474341No Hit
CCAAGTCAACAACCCCCACAAAATGTTTAATTTAACTGACCTTAAAATTT530.10114696845359644No Hit
CCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCATT530.10114696845359644No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)