FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39877
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG18214.5665421170098055No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACAGAGAGTCAAT16044.022368784010833No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC8452.1190159741204204No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC8192.0538154826090227No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA7721.9359530556461118No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA7591.9033528098904129No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT7071.7729518268676179No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC7061.7704441156556412No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA6951.7428592923238961No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA6871.7227976026280813No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG6711.6826742232364522No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG6691.6776588008124986No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT6681.6751510896005215No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC6571.6475662662687764No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT6181.54976552900168No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT6141.539734684153773No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA6131.537226972941796No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG6001.5046267271860974No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC5931.4870727487022595No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT5901.479549615066329No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG5901.479549615066329No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG5751.4419339468866765No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGATGGC5281.3240715199237656No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT5181.2989944078039974No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA5041.2638864508363217No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT5041.2638864508363217No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT4971.2463324723524838No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG4541.1385008902374802No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT4511.1309777566015498No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG4181.0482232866063144No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC4081.0231461744865462No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGAAAAGATGCTGAGG4061.0181307520625924No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT3970.995561351154801No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA3890.9754996614589864No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG3810.9554379717631718No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA3720.9328685708553802No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT3620.907791458735612No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA3610.9052837475236352No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTACAGAGAGTCAATCTCG3480.8726835017679364No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC3160.7924367429846779No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG2930.7347593851092109No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG2850.7146976954133962No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC2770.6946360057175816No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA2650.6645434711738596No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC2440.6118815357223463No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA2360.5918198460265316No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG2310.5792812899666475No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG2060.5165885096672267No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTACAGAGAGT1980.4965268199714121No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACCTTTAT1980.4965268199714121No Hit
TTTCGCTTCCCACAGGTCTCTGCTAGGGGGCTGGGGTTGGGGTCGGGGTG1850.46392657421571337No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAC1590.39872608270431575No Hit
GCGTCTTCACCTGGAAGGGGTCCATGTGCCCCTCCTTCTGGCCACCATGC1480.37114125937257064No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC1280.32098703513303406No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA1220.30594076786117314No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACAGAGAGTCAAC660.1655089399904707No Hit
GCGTCTTCACCTGGACTATGTCCGGAGACCAAGTCTCTGCTACCGTACAG650.16300122877849385No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACAGAGAGTCAAT620.1554780951425634No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG560.1404318278707024No Hit
ATACGGCCAGGCATTGAAGTTTCATGGAAGCCAGCCCCTCAGGGCAACTG550.13792411665872556No Hit
GTAGAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTACAG530.13290869423477195No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACAGAGAGTCAAG460.11535471575093413No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTACAGAGAGTCAAA460.11535471575093413No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTACAG430.10783158211500364No Hit
GGGGTCAGGAGGAGACCAAGTCTCTGCTACCGTACAGAGAGTCAATCTCG430.10783158211500364No Hit
TTTCGCTTCCCACAGGTCTCTGCTAGGGGGCTGGGGTTGGGGTGGGGGTG420.1053238709030268No Hit
TCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA420.1053238709030268No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTACAGAGAGTCAATCT410.10281615969104999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)