FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38130
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC18304.799370574350905No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG13453.527406241804353No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT11933.1287699973773933No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT10332.7091528979805926No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG8882.3288749016522425No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT8362.1924993443482825No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA7882.0666142145292423No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGTAATGGAGTAT7812.048255966430632No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT7421.9459742984526618No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG6631.7387883556254917No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA6591.728297928140572No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC6531.7125622869131916No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA6231.6338840807762915No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC5971.5656963021243113No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT5721.5001311303435616No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC5681.4896407028586416No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG5591.4660372410175715No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT5231.3716233936532913No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA5181.3585103592971415No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC5161.3532651455546814No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT5081.3322842905848413No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA5001.3113034356150013No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG4731.2404930500917912No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA4561.195908733280881No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA4521.185418305795961No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG4471.1723052714398112No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA4411.1565696302124313No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC4351.1408339889850512No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA3851.009703645423551No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT3780.9913453973249411No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG3640.9546289011277209No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT3510.9205350118017309No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC3490.9152897980592709No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA3490.9152897980592709No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTGTTCATACCAGGACCA3240.8497246262785209No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT2900.7605559926567007No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG2810.7369525308156308No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG2780.7290847102019408No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC2520.6608969315499607No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC2500.6556517178075006No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACCTTTAT2290.6005769735116706No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG2290.6005769735116706No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG2180.5717282979281406No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG2150.5638604773144507No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTAGTAATGGAGTATCTCG2100.5507474429583006No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG1870.49042748492001054No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA1840.48255966430632047No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC1510.39601363755573044No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT1470.38552321007081036No Hit
GTAGAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTAGTA1190.3120902176763703No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTAGTAATGGAGTATCT1150.3015997901914503No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTAGTAATGGA1110.2911093627065303No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTAGTA900.23603461841070023No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC740.19407290847102018No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC740.19407290847102018No Hit
TTTCGCTTCCCACAGGTCTCTGCTAGGGGGCTGGGGTTGGGGTCGGGGTG700.1835824809861002No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAG700.1835824809861002No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG690.1809598741148702No Hit
GGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTAGTAATGGA640.16784683975872017No Hit
GCAGCTTCTGCCATCTCTCTCTTCCTTTTTCTTCAGCCACAGGCTCCCAG640.16784683975872017No Hit
AGCTGCTCACCATCGCTACCGTAGGTCTTGGCCAGTTGGAGACCAAGTCT520.13637555730396014No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA520.13637555730396014No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGTAATGGAGTAT500.13113034356150013No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT460.12063991607658012No Hit
GGGGTCAGAGGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCT440.11539470233412011No Hit
CAGTCAGAGGCGCTATGTGTATTACTATAGCTACCTGTTAAAGAATCATC440.11539470233412011No Hit
GGGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTAGT430.11277209546289012No Hit
TTTCGCTTCCCACAGGTCTCTGCTAGGGGGCTGGGGTTGGGGTGAGGGTG430.11277209546289012No Hit
GGAAACCGTAGCTGCCCTGTAGGTTTTCTGGGAAGGGACAGAAGATGACA390.1022816679779701No Hit
CAGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTAGT390.1022816679779701No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)