FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008100949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008100949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37705
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC16134.277947221853866No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG11292.99429783848296No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT11122.9492109799761304No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATATGGTAAGGAT10462.7741678822437343No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT9952.638907306723246No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG9022.3922556690094154No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT8612.2835167749635326No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT8142.1588648720328867No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA7892.092560668346373No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA7341.9466914202360428No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC6591.7477788091765016No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA6301.6708659329001458No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC5971.5833443840339478No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT5511.4613446492507625No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC5431.440127304071078No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC5361.4215621270388543No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG5151.3658665959421827No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT5001.3260840737302744No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG4991.323431905582814No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT4971.3181275692878929No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA4851.2863015515183662No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG4841.2836493833709057No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA4721.2518233656013793No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG4401.1669539848826416No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA4291.1377801352605754No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA4291.1377801352605754No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA4021.0661715952791406No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC3981.0555629226892986No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT3881.029041241214693No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA3861.0237369049197718No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG3821.0131282323299298No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT3610.9574327012332582No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG3470.9203023471688104No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA3380.8964328338416656No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC3170.840737302744994No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTGTTCATACCAGGACCA3110.8248242938602308No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTATATGGTAAGGATCTCG2940.7797374353534013No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT2740.7266940724041904No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC2400.6365203553905318No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC2390.6338681872430713No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG2330.6179551783583079No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG2230.5914334968837024No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACCTTTAT2190.5808248242938603No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG2160.5728683198514786No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG2130.5649118154090969No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG2000.5304336294921098No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA1920.5092162843124254No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT1600.4243469035936878No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTATAT1480.3925208858241613No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC1460.3872165495292402No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTATATGGTAA1300.34478185916987136No Hit
GTAGAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTATAT1030.27317331918843657No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG900.2386951332714494No Hit
GGAAACCGTAGCTGCCCTGTTAAAGAATCATCTGGACCTGGGTCTTAGAC890.23604296512398887No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC890.23604296512398887No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTATATGGTAAGGATCT890.23604296512398887No Hit
TTTCGCTTCCCACAGGTCTCTGCTAGGGGGCTGGGGTTGGGGTCGGGGTG780.2068691155019228No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAT770.20421694735446227No Hit
GGGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTATA570.1511735844052513No Hit
CAGTCAGAGGCGCTATGTGTATTACTATAGCTACCTGTTAAAGAATCATC550.1458692481103302No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACATGGTTCGACAGGGGCCAG540.14321707996286964No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATATGGTAAGGAC540.14321707996286964No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATATGGTAAGGAT510.135260575520488No Hit
GGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTATATGGTAA510.135260575520488No Hit
GGTCTTGGCCAGTTGGAGACCAAGTCTCTGCTACCGTATATGGTAAGGAT490.1299562392255669No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATATGGTAAGGAA460.12199973478318525No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA450.1193475666357247No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTATACGGAAGGCAGTCTGGCTG410.10873889404588251No Hit
TCCACAAACAGAACAAGATGCTAGACCAAGTCTCTGCTACCGTATATGGT400.10608672589842195No Hit
CAGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTATA390.1034345577509614No Hit
GGGGTCAGGAGGAGACCAAGTCTCTGCTACCGTATATGGTAAGGATCTCG380.10078238960350087No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)