FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008101383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008101383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86106
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC77448.99356606972801No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG70908.234037117041785No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC66957.775300211367385No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA64187.453603697767868No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC63047.321208742712471No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC38634.4863308015701575No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG35294.0984368104429425No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT32133.731447285903421No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC26663.096183773488491No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA25712.98585464427566No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG24552.8511369707105194No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT17031.9777948110468493No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT13831.6061598494878406No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG12481.4493763500801338No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA11391.3227881912990964No Hit
GGAAACCGTAGCTGCCCTGGGTCTTAGACCAAGTCTCTGCTACCGTATTG10081.1706501289108773No Hit
GGTCTTCTCTGTTTCAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTG9021.0475460478944556No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG6800.7897242933128934No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC5560.6457157457087775No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA4820.5597751608482568No Hit
GCAACAGAGGAGGTGGCTTTAGGTCAGCAGACCAAGTCTCTGCTACCGTA4180.48544816853645506No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT4000.4645437019487608No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC3900.4529301094000418No Hit
AGCTGCTCACCATCGCTACCGGAAGGTCTTGGCCAGTTGGAGACCAAGTC2760.32053515434464497No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACGCC2560.2973079692472069No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA1920.2229809769354052No Hit
AGCTGCTCACCATCGCTAGCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC1860.21601282140617378No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA1810.21020602513181427No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGCTCAACTTCTGTAACACC1710.19859243258309525No Hit
GCGTCTTCACCTGGACTATGTCCGGAGACCAAGTCTCTGCTACCGTATTG1610.18697884003437623No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT1410.1637516549369382No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATTGTCGAGACAT1310.15213806238821917No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACCTTACTTTCATTG1250.14516990685898776No Hit
ATACGGCCAGGCATTGAAGTTTCATGGAAGCCAGCCCCTCAGGGCAACTG1170.13587903282001254No Hit
GGAAACCGTAGCTGCCCTGGGTTTCATCTGGACCTGGGTCTTAGACCAAG1070.12426544027129352No Hit
AGGACAAAATGTTTCACTTTTGGTAAATACATTCTTCATACCAGGACCAG970.1126518477225745No Hit
GCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTCT890.10336097368359928No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTAGAGACCAAGTC870.10103825517385548No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)