FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008101384

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008101384
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86106
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA76948.935498106984415No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC69858.112094395280236No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA58216.760272222609343No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT47295.4920679162892245No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC45865.325993542842543No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA32763.804612918960351No Hit
GGCCTTTTCCTTCAAACAGGATTATTTACCCTTATAATGTCTCACCAATG31913.7058973822962393No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG25182.9243026037674493No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG22502.6130583234617797No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT21672.5166655053074116No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA20742.408659094604325No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC16651.9336631593617168No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG12371.4366013982765429No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA11161.2960769284370426No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC11021.279817898868836No Hit
AAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA9771.1346479920098482No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA8991.04406197012984No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT8180.9499918704852159No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAACGCTATC7760.901214781780596No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC7040.817596915429819No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG6230.723526815785195No Hit
CTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT4970.5771955496713354No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAATTCAGACTTTTGTAATT4930.5725501126518477No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCCC4890.5679046756323601No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT4170.4842868092815832No Hit
GGCCTTTTCCTTCAAACAGGATTATTTACCCTTATAATGTCTCACCAACG4070.4726732167328641No Hit
GCTGACCTAAAGCCACCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGT4040.46918913896824843No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG3890.45176875014516993No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAAATTCAGACTTTTGTAA3570.41460525398926906No Hit
CCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG3460.4018303021856781No Hit
CGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG3170.368150883794393No Hit
TCTAGATATGGTTAAGAAAACTGTTCCAATACATGGAAGGATGAGAATTT3140.36466680602977725No Hit
CCAACTGGCCAAGACCTTCCGGTAGCGATGGTGAGCAGCTTGTAGAACCA2760.32053515434464497No Hit
CCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT2420.2810489396790003No Hit
CCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA2260.26246719160104987No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT1870.21717418066104568No Hit
CCAACTGGCCAAGACCTTACGCTAGCGATGGTGAGCAGCTTGTAGAACCA1830.21252874364155808No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTACAAATGCTATC1680.19510835481847955No Hit
CCGGACATAGTCCAGGTGAAGACGCTGTAGAACCATGTCGTCAGTGTAGA1590.18465612152463243No Hit
GGCCTTTTCCTTCAAACAGGATTATTTACCCCTATAATGTCTCACCAATG1470.1707198104661696No Hit
CGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA1400.1625902956820663No Hit
TCCAGGAAGAGGAAAGGAAAAACATCAAAAAATAACTTACCTTTTTGTCT1360.15794485866257868No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG1350.15678349940770678No Hit
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAACGCTATC1320.15329942164309107No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTT1210.14052446983950015No Hit
AAGACCCAGGTCCAGATGAAACCCAGGGCAGCTACGGTTTCCTGTAGAAC1030.11962000325180591No Hit
CAGACCCAGGGCAGCTACGGTTTCCTGTAGAACCATGTCGTCAGTGTAGA910.10568369219334309No Hit
CTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTTG900.10452233293847119No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTGTAGAACCAT880.10219961442872738No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)