FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008101390

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008101390
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141268
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC51993.680238978395674No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA42382.9999716850242093No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC37672.6665628450887673No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC34942.4733131353172695No Hit
GGCCTTTTCCTTCAAACAGGATTATTTACCCTTATAATGTCTCACCAATG32382.292097290256817No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG30692.1724665175411277No Hit
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG28332.0054081603760228No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA27291.931789223320214No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG27261.929665600135912No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA24621.7427867599173203No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC24561.7385395135487158No Hit
AAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT24101.705977291389416No Hit
AATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT23371.654302460571396No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC22181.5700654075940765No Hit
CTGATGGGACCCACTCCATCGAGATTTCACTGTAGCTAGACCAAAATCAC21711.536795311040009No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA21601.529008692697568No Hit
GCCTCAAAGACAATGGCTCCTGGTTGTAGCTAACTAACTTCAGAACACCA21351.511311832828383No Hit
TCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCATT20311.437692895772574No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT20301.4369850213778066No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG20281.435569272588272No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT20161.4270747798510632No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT18401.302488886372002No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG18101.2812526545289804No Hit
TCTCCCAATGAAAGTAAAGTACAAACCTTTTTTAGCATCTTGTTCTGTTT18061.2784211569499109No Hit
ATGAAAACACAACATGAATATAAACATCAATATTTGAAATAGAAAATCAA17281.2232069541580541No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC17021.204802219894102No Hit
CCACCAGTCACTCACACTTGACCATGATCATGTAGACATCGATGGTACAT16101.1396777755755019No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT16061.1368462779964323No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA15661.1085313022057366No Hit
ACAAGTCAACAACCCCCACAAAATGTTTAATTTAACTGACCTTAAAATTT15481.0957895630999237No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT14991.0611037177563212No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA14621.034912365149928No Hit
TCCAGGAAGAGGAAAGGAAAAACATCAAAAAATAACTTACCTTTTTGTCT14080.9966871478324886No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTT13180.9329784523034232No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGGTCCATAGAAAATCTTT13130.9294390803295863No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC12500.8848429934592407No Hit
GGTCTTTGCCTGCTGAGAGTTATTAACAGTGCAGTGTGGAATCCAGAGTG11810.8359996602202905No Hit
TCTAGATATGGTTAAGAAAACTGTTCCAATACATGGAAGGATGAGAATTT11400.8069768100348275No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG11280.7984823172976188No Hit
GGCCTCAGTACAAACTCATTAGCATCAGGATTATGACTCACCGTAGCTCC10640.7531783560325056No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT10210.7227397570575077No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT9010.6377948296854207No Hit
CCATGGGACTGACTTTCTGCTCTTGTCTTTCAGACTTCCTGAAAACAACG8790.6222215930005379No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGAC8720.6172664722371662No Hit
CAGCCAGACTGCCTTCCGGGTCACTGCCATGGAGGAGCCGCAGTCAGATC8270.5854121244726336No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGACAGAGATTTCATA6390.45233173825636375No Hit
TCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTCT6210.4395899991505507No Hit
TCCGTCTACTCCCACGTTCTAAGAGAGTGACAGAAAGGTAAAGAGGAGCA5900.4176458929127615No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC5060.3581844437523006Illumina Single End PCR Primer 1 (100% over 21bp)
CCTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC4780.3383639606988136No Hit
CCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG3560.25200328453719173No Hit
CCATGGGACTGACTTTCTGCTCTTTTCACCCATCTACAGTCCCCCTTGCC3470.24563241498428517No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA3250.23005917829940253No Hit
CGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG2510.1776764730866155No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA2190.15502449245405897No Hit
CCCCCTCCTCTGTTGCTGTAGATCCGTGGGCGTGAGCGCTTCGAGATGTT2180.15431661805929156No Hit
CCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA2180.15431661805929156No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAATTCAGACTTTTGTAATT2100.14865362290115242No Hit
CCAAGTCAACAACCCCCACAAAATGTTTAATTTAACTGACCTTAAAATTT2090.14794574850638503No Hit
CCCAGGAAGAGGAAAGGAAAAACATCAAAAAATAACTTACCTTTTTGTCT1740.12317014468952629No Hit
CCTGGATCAGAGTCAGTGGTGTCAGAATATCTATAATGATCAGGTTCATT1730.1224622702947589No Hit
TGCCCTGACTTTCAACTCTGTGTAGAACCATGTCGTCAGTGTAGATCTCG1710.12104652150522412No Hit
CGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA1660.11750714953138715No Hit
CTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT1610.11396777755755018No Hit
CAATGGAAGCACAGACTGCAATTTGTACCTGAATGACAAGGTAGCGCTGG1530.10830478239941105No Hit
CTGAAAACACAACATGAATATAAACATCAATATTTGAAATAGAAAATCAA1520.10759690800464365No Hit
CAGGGTGCAGTTATGCCTCAGATTCACTTTTATCACCTTTCCTTGCCTCT1500.10618115921510887No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAAATTCAGACTTTTGTAA1500.10618115921510887No Hit
CCCCCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG1480.10476541042557408No Hit
CATAGTTGTTTTAGAAGATATTTGCAAGCATACAAATAAGAAAACATACT1480.10476541042557408No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)