FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008101391

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008101391
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126164
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA59454.712120731745982No Hit
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG53294.223867347262294No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA42723.3860689261596013No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT41003.2497384356868837No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC32612.584730985067056No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT30472.4151104911068133No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC29242.317618338036207No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT26892.1313528423322023No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA24221.9197235344472274No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT23361.8515582892108684No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT23261.843632097904315No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG22491.782600424843854No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG22361.7722963761453345No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC21831.7302875622206018No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA21431.6985827969943883No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG21271.685900890903903No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT21201.6803525569893154No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA20761.6454773152404807No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA20221.6026758821850922No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA20111.5939570717478837No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG18921.4996353951998986No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC18911.498842776069243No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT17801.4108620525665008No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC17141.3585491899432485No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT16811.3323927586316224No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG16561.312577280365239No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC16321.293554421229511No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT15671.242034177736914No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA15661.2412415586062586No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAGACGTGCTTCAT13841.096984876826987No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG13591.0771693985606037No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG12891.0216860594147301No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC12691.0058336768016234No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC11800.9352905741732982No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG11160.8845629498113566No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG10720.8496877080625218No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC8890.7046384071525951No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA8770.695126977584731No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT8440.6689705462731048No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA8400.6658000697504834No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG7950.6301322088709933No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTCTTGGCCAGTTGG7320.5801972036397071No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT6980.5532481531974256No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTAGAC4920.3899686122824261No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC4160.3297295583526204No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC3730.2956469357344409No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCAAGGTCTTGGCCAGTTGG3670.29089122095050884No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG3500.2774166957293681No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGAGGTGGGAGGCTG3030.24016359658856729No Hit
CCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA2790.22114073745283916No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGTAGCGCCT2700.21400716527694114No Hit
TTTCGCTTCCCACAGGTCTCTGCTAGGGGGCTGGGGTTGGGGTCGGGGTG2660.21083668875431977No Hit
GGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA2250.17833930439745094No Hit
GGGGTCAGAGGCAAGCAGGAGGAGATAACACAGGCCCAAGAGACCAAGTC2230.17675406613614025No Hit
CTGGTGTTGTTGGGCAGTTCGTGGTGAGGCTCCCCTTTCTTGCGGAGATA2040.16169430265368887No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGAGTCTTCAGTGAAC2000.1585238261310675No Hit
GGGGTCAGAGGCAAGCAGAGGAGGAGATAACACAGGCCCAAGAGACCAAG1950.15456073047779081No Hit
GGAAACCGTAGCTGCCCTGGTAGGTTTTCTGGGAAGGGACAGAAGATGAC1900.15059763482451413No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCAACAGAGGA1680.13316001395009672No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCCA1650.1307821565581307No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAG1600.126819060904854No Hit
GGGGTCAGAGGCAAGCAGGAGATAACACAGGCCCAAGAGACCAAGTCTCT1540.12206334612092197No Hit
GCGTCTTCACCTGGACTATGTCCGGAGACCAAGTCTCTGCTACCGTAGAC1360.10779620176912591No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATTGATAGC1350.10700358263847057No Hit
TGTGCTGTGACTGCTTGTAGGTGGCCATGGCGCGGACGCGGGTGCCGGGC1300.10304048698519387No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA1290.10224786785453853No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTAGACGTGCTTCATCT1280.10145524872388319No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)