FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008101399

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008101399
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37485
Sequences flagged as poor quality0
Sequence length125
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT33228.862211551287182No Hit
GGAATCCTATGGCTTTCCAACCCAGCAACAGAGGAGGGGGAGACCAAGTC21095.62625050020008No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA20325.4208350006669335No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC19405.175403494731226No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCAACAGAGGA18975.060690943043884No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA18124.833933573429372No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATCCTGACAGAAT16334.3564092303588104No Hit
TGTGCTGTGACTGCTTGTAGAACCAACAGAGTTGAAAGTCAGGGCAAGAC14603.8948912898492734No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC11433.04921968787515No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC9022.406295851674003No Hit
TCTGTATCAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGT7812.0835000666933436No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG6521.739362411631319No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC6421.7126850740296118No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG5601.4939309056956116No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT5311.4165666266506602No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAT4511.2031479258370015No Hit
CAGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTATC2960.7896491930105375No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTATCCTGACAGAATCT2940.7843137254901961No Hit
GCGTGGACAACCCCCACGCACTGACGACATGGTTCTACAGCAACAGAGGA2900.7736427904495131No Hit
GCAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTATCC2760.7362945178071229No Hit
TGTGCTGTGACTGCTTGTAGAACCAACAGAGTTGAAAGTCAGGGCGAGAC2230.5949046285180739No Hit
GGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA2170.5788982259570495No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATATATTCCTCCAATTCAGGACCC2130.5682272909163666No Hit
GCGTCTTCACCTGGACTATGTCCGGAGACCAAGTCTCTGCTACCGTATCC1710.4561824729891957No Hit
AGCTGCTCACCATCGCTACACTGACGACAGGTCTTGGCCAGTTGGAGACC1680.44817927170868344No Hit
GCGTGGACAACCCCCACCACTGACTCTGATCCAGAAGACCAAGTCTCTGC1540.4108309990662932No Hit
TCATAATGCTTGCTCTGATAGGAGTGGGTCCCATCAGAGACCAAGTCTCT1230.3281312525010004No Hit
TGACAGTTTGACAGTTAAAGGTGAAGATGTATTCCTCCAATTCAGGACCC1200.3201280512204882No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC1100.29345071361878083No Hit
GGGGTCAGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACC1100.29345071361878083No Hit
TCCCTGCCAGCGAGATACACTGACGACATGGTTCTACATCCCTCCATGAG1070.28544751233826865No Hit
TCCACAAACAGAACAAGATGCTAGACCAAGTCTCTGCTACCGTATCCTGA1050.2801120448179272No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATCCTGACAGAAT1040.2774443110577564No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA1000.26677337601707346No Hit
GGGAGGGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTATC880.23476057089502467No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTATCCTGACAGAATCTCG870.23209283713485396No Hit
GCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTATCCTGACAGAA850.22675736961451248No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA850.22675736961451248No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT830.221421902094171No Hit
GCGTGGACAACCCCCATACACTGACGACATGGTTCTACAGCAACAGAGGA740.19741229825263437No Hit
GGGGTCGGAGATAACACAGGCCCAAGAGACCAAGTCTCTGCTACCGTATC730.19474456449246366No Hit
CAGAGGGGGGGGAGACCAAGTCTCTGCTACCGTATCCTGACAGAATCTCG670.17873816193143927No Hit
CAACAGAGCAGTCAGAGGACCAGGAGACCAAGTCTCTGCTACCGTATCCT660.17607042817126853No Hit
TCTGTATCAGGCAAAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTAC640.17073496065092705No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTCTTGGCCAGTTGG620.16539949313058558No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG610.16273175937041484No Hit
GGTCTTGGCCAGTTGGAGACCAAGTCTCTGCTACCGTATCCTGACAGAAT610.16273175937041484No Hit
GCAACAGAGGACCAGGAGACCAAGTCTCTGCTACCGTATCCTGACAGAAT580.15472855808990263No Hit
TCATAATGCTTGCTCTGATGGAGTGGGTCCCATCAGAGACCAAGTCTCTG580.15472855808990263No Hit
GCAACAGGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTATCCTGACAGA570.1520608243297319No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATCCTGACAGAAC510.13605442176870747No Hit
TCTTAAATGGCTACCTGTTAAAGAATCATCTGGAAGACCAAGTCTCTGCT470.12538348672802455No Hit
GTAGAGGAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTATCC460.1227157529678538No Hit
GGAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTATCCTGACAGAAT440.11738028544751233No Hit
AGCTGCTCACCATCGCTACACTGACGACAGGGGCCAGTTGGAGACCAAGT410.10937708416700012No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTAGAC410.10937708416700012No Hit
TCATAATGCTTGCTCTGATAGGAGATAACACAGGCCCAAGAGACCAAGTC400.10670935040682938No Hit
GCGTGGACAACCCCCACACTGACGACATGGTTCTACAGCAACAGAGGAGG400.10670935040682938No Hit
TCTGTATCAGCCATTGTCTTTGAGGCAGACCAAGTCTCTGCTACCGTATC390.10404161664665866No Hit
GGACCAGAGGAAACAGACCAAGTCTCTGCTACCGTATCCTGACAGAATCT380.10137388288648792No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)