FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008101400

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008101400
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37485
Sequences flagged as poor quality0
Sequence length125
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG27407.309590502867813No Hit
CCCCCTCCTCTGTTGCTGGGTTGGAAAGCCATAGGATTCCTGTAGAACCA20815.5515539549153No Hit
GTTTCCTCTGGTCCTGGTATGAAGAATGTATTTACCCAAAAGTGAAACAT19115.098039215686274No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTG18404.908630118714152No Hit
CCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG17424.647192210217421No Hit
TGCCCTGACTTTCAACTCTGTTGGTTCTACAAGCAGTCACAGCACATGTA14313.8175270108043216No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT13203.5214085634253705No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC11263.0038682139522472No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA9402.5076697345604906No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATCGCTGCAACA7982.1288515406162465No Hit
AGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA7722.0594904628518074No Hit
GCCTCAAAGACAATGGCTCCTGATACAGATGTAGAACCATGTCGTCAGTG7722.0594904628518074No Hit
GCCTCTTGCTTCTCTTTTCCTATCCTGAGTAGTGGTAATCTACTGGGACG5171.37921835400827No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTA4931.3151927437641724No Hit
TCTAGATATGGTTAAGAAAACTGTTCCAATACATGGAAGGATGAGAATTT4481.1951447245564892No Hit
TCCCTGCTTCTGTCTCCTACAGCCACCTGAAGTCCAAAAAGGGTCAGTCT4041.077764439108977No Hit
CCCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG3831.0217420301453914No Hit
GTTTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC2980.7949846605308791No Hit
CTTGGGCCTGTGTTATCTCCCCTCTGTGTAGAACCATGTCGTCAGTGTAG2940.7843137254901961No Hit
GGCTCCTTATCTCCCCTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGCG2860.7629718554088302No Hit
CCTGGTCCTCTGACTGCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGA2720.7256235827664399No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAGTACATGAACTTGTCTTCC2550.6802721088435374No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGGTCCATAGAAAATCTTT2370.6322529011604642No Hit
CGCCCTGACTTTCAACTCTGTTGGTTCTACAAGCAGTCACAGCACATGTA2210.5895691609977325No Hit
AACAGAGAATCTCCATTTTAGCACTTACCTGTGACTCCATAGAAAATCTT2110.5628918233960251No Hit
CCAACTGGCCAAGACCTGTCGTCAGTGTAGCGATGGTGAGCAGCTTGTAG1710.4561824729891957No Hit
CCGGACATAGTCCAGGTGAAGACGCTGTAGAACCATGTCGTCAGTGTAGA1680.44817927170868344No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAATTCAGACTTTTGTAATT1620.43217286914765907No Hit
TCTGGATCAGAGTCAGTGGTGGGGGTTGTCCACGCTGTAGAACCATGTCG1560.41616646658663464No Hit
CACACACAGGTAACGGCTGAGGGAACTCAAAATACATGAACTTGTCCTCC1300.3468053888221956No Hit
CTCATGGTGTTTTATCCCTCTTGATAAAAAAAAATTCAGACTTTTGTAAT1280.3414699213018541No Hit
CTGATGGGACCCACTCCTATCAGAGCAAGCATTATGATGTAGAACCATGT1280.3414699213018541No Hit
TCCCTGCTTCTGTCTCCTACCGCCACCTGAAGTCCAAAAAGGGTCAGTCT1210.3227957849806589No Hit
CGAATGGTCCTGCACCAGTAATATGCATATTAAAACAAGATTTACCTCTA1160.30945711617980526No Hit
CCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGCAACA1130.3014539148992931No Hit
CCCCCTCCTCTGTTGATGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG1090.2907829798586101No Hit
CTTGGGCCTGTGTTATCTCCCCTCTGACCCCTGTAGAACCATGTCGTCAG1060.2827797785780979No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC1050.2801120448179272No Hit
AGCATCTTGTTCTGTTTGTGGATGTAGAACCATGTCGTCAGTGTAGATCT1010.2694411097772442No Hit
CCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATCGCTGCAACA1000.26677337601707346No Hit
CCTCCTGGCCCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC940.2507669734560491Illumina Single End PCR Primer 1 (100% over 21bp)
CTTGGGCCTGTGTTATCTCCCCTCCCTGTAGAACCATGTCGTCAGTGTAG840.22408963585434172No Hit
CCTGGTCCTCTGACTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTG830.221421902094171No Hit
TCATTACACCAGTTCGTCCCTTTCCAGCTTTACAGTGAATTGCTGTAACA800.21341870081365877No Hit
CTCGATAATCTGGATGACTCATTATTGTTATGACAGTAAGATACAGTCTA800.21341870081365877No Hit
AGGGATGCAAAGGCCTCATGGAGGGATGTAGAACCATGTCGTCAGTGTAT800.21341870081365877No Hit
TTCATGGTGTTTTATCCCTCTTGATAAAAAAAAAATTCAGACTTTTGTAA750.20008003201280514No Hit
TCTAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTG740.19741229825263437No Hit
CTTGGGCCTGTGTTATCTCCGACCCCTGTAGAACCATGTCGTCAGTGTAG720.19207683073229292No Hit
CCCCCCCCTCTGTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTCGC690.1840736294517807Illumina Single End PCR Primer 1 (95% over 22bp)
CCAACTGGCCAAGACCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG680.18140589569160998No Hit
CCAGATGATTCTTTAACAGGTAGCTATAATAATACACATAGCGCCTCTGA650.1734026944110978No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT640.17073496065092705No Hit
CCAACTGGCCAAGACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCT630.16806722689075632No Hit
CCTGGTCCTCTGACTGCTCTGTTGTGTAGAACCATGTCGTCAGTGTAGAT620.16539949313058558No Hit
CCTGGTCCTCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG620.16539949313058558No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG600.1600640256102441No Hit
GCCTCAAAGACAATGGCTTTGCCTGATACAGATGTAGAACCATGTCGTCA580.15472855808990263No Hit
CTGATGGGACCCACTCCATCAGAGCAAGCATTATGATGTAGAACCATGTC560.14939309056956115No Hit
CCTCCTGGCCCCTGTTGCTGTAGAACCATGTCGTCAGTGTAGATCTCGGT560.14939309056956115No Hit
TCCAGATGATTCTTTAACAGGTAGCCATTTAAGATGTAGAACCATGTCGT480.12805122048819528No Hit
GCCTCAAAGACAATGGCTCCTCTACTGTAGAACCATGTCGTCAGTGTAGA460.1227157529678538No Hit
CCCCCTCCTCTGTTCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGG440.11738028544751233No Hit
CTTGGGCCTGTGTTATCTCCTATCAGAGCAAGCATTATGATGTAGAACCA420.11204481792717086No Hit
GTTTCCTCTGGTCCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGTC410.10937708416700012No Hit
GCCTCAAAGACAATGGCTGATACAGATGTAGAACCATGTCGTCAGTGTAG400.10670935040682938No Hit
CCAACTGGCCCCTGTCGTCAGTGTAGCGATGGTGAGCAGCTTGTAGAACC390.10404161664665866No Hit
AGCATCTTATCCGAGTGGAAGGAAATTTGCGTGTGGAGTATTTGGATGTA390.10404161664665866No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC380.10137388288648792No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)