FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008101465

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008101465
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139072
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACGGCCAGGCATTGAAGTCTCATGGAAGCCAGCCCCTCAGGGCAACTG51713.7182179015186376No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAATTAGCGAGTAT48683.5003451449608836No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCTGAAAGACATTATGACA39292.8251553152323976No Hit
AGGACAAAATGTTTCACTTTTGGGTAAATACATTCTTCATACCAGGACCA37472.694287850897377No Hit
TCTTAAATGGCTACGACCCAGTTACCATAGCAATTTAGTGAAATAACTAT30312.179446617579383No Hit
ACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGAT30132.1665036815462493No Hit
GCCTGCTGAAAATGACTGAATATAAACTTGTGGTAGTTGGAGCTGGTGGC29282.105384261389784No Hit
TCCAAATACTCCACACGCAAATTTCCTTCCACTCGGATAAGATGCTGAGG28362.0392314772204325No Hit
AGCTGCTCACCATCGCTATCTGAGCAGCGCTCATGGTGGGGGCAGCGCCT27591.983864473078693No Hit
GTAGAGGAGCCGTCAAATCCAGAGGCTAGCAGTTCAACTTCTGTAACACC26891.9335308329498389No Hit
CAGAGGGGAAAAATATGACAAAGAAAGCTATATAAGATATTATTTTATTT26351.8947020248504371No Hit
AGAAAACGGCATTTTGAGTGTTAGACTGGAAACTTTCCACTTGATAAGAG26021.8709733087896918No Hit
TCCACAAACAGAACAAGATGCTAAAAAAGGTTTGTACTTTACTTTCATTG25971.8673780487804876No Hit
AATCTGTCTTTTGGTTTTTCTTGATAGTATTAATGTAATTTCAAATGTTA23911.7192533364012885No Hit
TGTGCTGTGACTGCTTGTAGATGGCCATGGCGCGGACGCGGGTGCCGGGC23271.673234008283479No Hit
CAGCCTCTGGCATTCTGGGAGCTTCATCTGGACCTGGGTCTTCAGTGAAC22531.6200241601472618No Hit
TTCTTATTCTGAGGTTATCTTTTTACCACAGTTGCACAATATCCTTTTGA22061.5862287160607453No Hit
GGGGTCAGAGGCAAGCAGAGGCTGGGGCACAGCAGGCCAGTGTGCAGGGT21421.540209387942936No Hit
GGAATCCTATGGCTTTCCAACCTAGGAAGGCAGGGGAGTAGGGCCAGGAA20541.476932811780948No Hit
TCATAATGCTTGCTCTGATAGGAAAATGAGATCTACTGTTTTCCTTTACT19521.403589507593189No Hit
TGTGGTCTGCCAGCTAAAGGTGAAGATATATTCCTCCAATTCAGGACCCA19311.3884894155545329No Hit
TCTGTATCAGGCAAAGTCATAGAACCATTTTCATGCTCTCTTTAACAATT19101.3733893235158767No Hit
TGACCAATGGCTAAGTGAAGATGACAATCATGTTGCAGCAATTCACTGTA18211.3093936953520477No Hit
TGCTGCATATTTCAGATATTTCTTTCCTTAACTAAAGTACTCAGATATTT18161.305798435342844No Hit
GACCCAAAACCCAAAATGGCAGGGGAGGGAGAGATGGGGGTGGGAGGCTG17981.29285549930971No Hit
TCACAATTGCCAGTTAACGTCTTCCTTCTCTCTCTGTCATAGGGACTCTG17871.2849459272894614No Hit
AGATGAGTCATATTTGTGGGTTTTCATTTTAAATTTTCTTTCTCTAGGTG17581.264093419236079No Hit
CCCCTGCTCCTATAGCCAAGAAGTGGAATAGCATCTCTACGGGCCATTCT17411.251869535204786No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTATGTGATCAAGAAATCGATAGC17411.251869535204786No Hit
TGACAGTTTGACAGTTAAAGGCATTTCCTGTGAAATAATACTGGTATGTA16101.1576737229636447No Hit
TCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCT15521.11596870685688No Hit
TGTTCATTCATGATCCCACTGCCTTCTTTTCTTGCTTCATCCTCTCAGGG14541.045501610676484No Hit
GCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCC14141.016739530602853No Hit
GGATGCAGAGCTTCTTCCCATGATGATCTGTCCCTCACAGCAGGGTCTTC13870.9973251265531523No Hit
CAGTCAGAGGCGCTATGTGTATTATTATAGCTACCTGTTAAAGAATCATC13630.9800678785089738No Hit
CTGGTGTTGTTGGGCAGTGCTAGGAAAGAGGCAAGGAAAGGTGATAAAAG11890.8549528301886792No Hit
TCAAATCATCCATTGCTTGGGACGGCAAGGGGGACTGTAGATGGGTGAAA11580.8326622181316153No Hit
GCAGCTTCTGCCATCTCTCTCCTCCTTTTTCTTCAGCCACAGGCTCCCAG11550.830505062126093No Hit
GAGAAAGCCCCCCTACTGCTCACCCGGAGGGCCACTGACAACCACCCTTA11010.7916762540266912No Hit
GCAACAGATAACTCAGATTGCCTTATAATAGTCTTTGTGTTTACCTTTAT10520.7564427059364933No Hit
TGAGCAAGAGGCTTTGGAGTATTTCATGAAACAAATGAATGATGCACATC9990.7183329498389324No Hit
TGTCCTGCTTGCTTACCTCGCTTAGTGCTCCCTGGGGGCAGCTCGTGGTG9750.7010757017947538No Hit
GTGTCACTCGTAATTAGGTCCAGAGTGAGTTAACTTTTTCCAACAGAGGG8680.6241371375977911No Hit
GGGGCCAGGAGGAGACCAAGTCTCTGCTACCGTAATTAGCGAGTATCTCG8460.6083179935572941No Hit
TACCCTCCATGAGGCACACCACCTGCATTCAGGAAAAGTGGATGAGATGT7810.5615796134376438No Hit
TTTCGCTTCCCACAGGTCTCTGCTAGGGGGCTGGGGTTGGGGTCGGGGTG3650.2624539806718822No Hit
GCGTGGACAACCCCCACACACTGACGACATGGTTCTACAGCAACAGAGGA3190.22937758858720664No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG3180.22865853658536583No Hit
CAGAGTTGAAAGTCAGGGCAAGACCAAGTCTCTGCTACCGTAATTAGCGA2990.21499654855039116No Hit
GGGGTCAGGAGGAGACCAAGTCTCTGCTACCGTAATTAGCGAGTATCTCG2530.1819201564657156No Hit
AGCTGCTCACCATCGCTACGGTAGCAGGTCTTGGCCAGTTGGAGACCAAG2530.1819201564657156No Hit
TCAAATCATCCATTGCTTGGTAGCAGAAAGTCAGTCCCATGGAGACCAAG2420.1740105844454671No Hit
TCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAATTAGCGAGTAT2390.17185342843994478No Hit
GCAACAGAGGAGGGGAGATAAGGAGCCAGACCAAGTCTCTGCTACCGTAA2260.16250575241601473No Hit
GCCTTCTTTAAGCAATGCCATCTTTATCATTTCTTCCAGTGTTCTAATTG2210.15891049240681085No Hit
GCAACAGAGGAGGGGGAGACCAAGTCTCTGCTACCGTAATTAGCGAGTAC1760.12655315232397607No Hit
TATATCACTTTTAAACTTTTCTTTTAGTTGTGCCGAAAGACATTATGACA1740.12511504832029452No Hit
GCGTGGACAACCCCCATACACTGACGACATGGTTCTACAGCAACAGAGGA1590.11432926829268292No Hit
GCGTCTTCACCTGGACTATGTCCGGAGACCAAGTCTCTGCTACCGTAATT1560.11217211228716062No Hit
TCCACAAACAGAACAAGATGCTAGACCAAGTCTCTGCTACCGTAATTAGC1450.1042625402669121No Hit
GCAACAGAGGAAACAGACCAAGTCTCTGCTACCGTAATTAGCGAGTATCT1400.10066728025770824No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)