FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008101559

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008101559
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109627
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG91808.37384950787671No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC84157.67602871555365No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC72096.575934760597297No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC60335.503206326908517No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTA47474.330137648571976No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG40423.6870478987840585No Hit
GAGTTTGATGCACAACTTCAAGGCACACTGACTTATGGTATCAAATAATA36693.346803251023927No Hit
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT34523.148859313855163No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT33843.0868307989820027No Hit
TCTAAGCATAGCATTCAATTTTGGCCACTACAAATCATTCTCCTTAAGAC33653.0694993021792074No Hit
CCTCAGGGCCTCAGAGAAAGGGTGGGGAAACTTGAAAGTAGACCAAGTCT32893.000173314968028No Hit
TTTGCAGACCTTCTCAGGAGTACACTGACGACATGGTTCTACATGCTAAT32012.9199011192498197No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA25762.3497860928420917No Hit
ATGAGCAAGTTGGGGTGTGGGGGTTGTTGACTTGTAGACCAAGTCTCTGC24122.200187909912704No Hit
GAGTTTGATGCACAACTTCAAGGCCACTGACTTATGGTATCAAATAATAA22902.0889014567579154No Hit
ACATGTACATAGGGTTTATATATCATCAGCTATGTACATGCAGAGACCAA16631.5169620622656828No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA14571.3290521495616956No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTAGTG13421.2241509847026737No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTT13341.2168535123646547No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA11561.0544847528437338No Hit
CTGTGGTGCCACCTAAGCTCTGCTACCGTACAGCAAAAAGTCCTGCAACT10350.9441104837311975No Hit
CTTAAATGTTGAAGGTGGTTCTTCAGCAAATTGGAAAGGTAAAGTAAAGA9210.8401215029144281No Hit
AGAGGAACGTTACACACCAGCAAATGATCAGCCTTGCAAAGACCAAGTCT9190.8382971348299233No Hit
TTAAATTTCACTCCACTTACCTACCAGTCAAATGTCCATGGATCATAGAC8940.8154925337736142No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT8610.7853904603792862No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC7720.7042060806188257No Hit
TTACAATTTCTACCGCTGGTTCCACACTGACGACATGGTTCTACATGAAA7700.702381712534321No Hit
GCAGAGCCAAACCGACCTGAGCATCCCTCAAATGGCACAGCTGCTTAACA7380.6731918231822452No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTAGTGGCTT7270.6631577987174693No Hit
TCCTCCCCTCTTAGCTGTCTTAAAGAATGGCAGACTGACAGTTGGTCAGA6710.6120754923513368No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA6360.580149050872504No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAGT6020.5491347934359236No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTAGTGG5810.5299789285486239No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC5700.519944904083848No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC5570.5080865115345672No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTAGTGGCT5410.49349156685852935No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG4600.4196046594360878No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTATACGGAAGGCAGTCTGGCTG4460.40683408284455475No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAG4360.397712242422031No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTAGTG4280.39041477008401215No Hit
TGGGTCCAGTCAGTGAACGAGATACTTTCCTGAGTGCCAGACCAAGTCTC4190.3822051137037409No Hit
TGCTAATTGGGCAGATAGAGCTTCGTTCAGACCAAGTCTCTGCTACCGTA4020.36669798498545064No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG3720.33933246371787973No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT3280.2991963658587757No Hit
AAGGGAAAAGGATGGATTAGGGTACACTGACGACATGGTTCTACTACCCT2990.2727430286334571No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTAGTGGC2950.26909429246444766No Hit
TGGTGATAGATGACTTGCTGCTACACTGACCCATGTGGAATAGTTCCCAG2920.26635774033769055No Hit
GTAACTTGATTTGCTGTTGTATTTGCTACCGTAACCCTCAATTTGGAAGC2680.24446532332363374No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTAGTGGC2640.2408165871546243No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG2570.23443129885885777No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTAGTGGCT2340.21345106588705337No Hit
ATGAGCAAGTTGGGGTGTGGGGGTTGTTGACTTGGAGACCAAGTCTCTGC2230.20341704142227737No Hit
CCTCAGGGCCTCAGAGAAAGGGTGGGGAAACTTGAAAGGAGACCAAGTCT2220.20250485738002502No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCGAGACCAAGT2220.20250485738002502No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA2160.1970317531265108No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTG2150.19611956908425843No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTAGTGG2130.1942952009997537No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC2110.19247083291524897No Hit
GCAATAAAACTAGTAGTGCAGATACCCAAAAAGTGGCCATTATTGAACTT2050.1869977286617348No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT2040.18608554461948243No Hit
CCAAATAACAAGTGTTGGAAGCAGGGAAGCTCTTCATCCTCACTAGATAA1850.16875404781668749No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTAGTGGCTTCGTATC1790.1632809435631733No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAGTG1640.14959818292938784No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT1630.14868599888713546No Hit
GTACAATTTCTACCGCTGGTTCCACACTGACGACATGGTTCTACATGAAA1540.1404763425068642No Hit
GAATTGTGCAGAGTGTGGTGTACCATGAAGAATCCCCACCACAATACCAA1530.13956415846461181No Hit
CCTTCCGTTCCAGTTACCGGGACAGGTCATTCTCTCCTGGCTCCTATGAG1260.11493518932379797No Hit
GGAAATTGAAGAAATCTGCCTAGTAGATGAGAACCAGTTCACCTTAACCA1240.11311082123929325No Hit
CAGTAGAGGCTAAGGATTCAGGTGTGGGGGTTGTTGACTTGTAGACCAAG1200.10946208507028378No Hit
TCTATTGTGATGGCCACGTTAGACCAAGTCTCTGCTACCGTAGTGGCTTC1190.10854990102803141No Hit
TGAATTCTCCTCAGATGACTCCATTTAAAGACCAAGTCTCTGCTACCGTA1170.10672553294352669No Hit
TCTAAGCATAGCATTCAATTTTGGCCACTACAAATCATTCTCCTTGAGAC1150.10490116485902196No Hit
AATCTCCAAGGAAGTTGTACCGTCTTAGACCAAGTCTCTGCTACCGTAGT1100.10034024464776012No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)