FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008101560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008101560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109627
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT90278.234285349412097No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG76286.95813987430104No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG72046.571373840386037No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA60415.510503799246536No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT46794.268109133698815No Hit
GAACGAAGCTCTATCTGCCCAATTAGCATGTAGAACCATGTCGTCAGTGT37483.4188657903618633No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG36733.350451987192936No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTGCCTTGAAGTTGTG36533.3322083063478884No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC33793.0822698787707408No Hit
TAAGGAGAATGATTTGTAGTGGCCAAAATTGAATGCTATGCTTAGATGTA33323.0393972287848796No Hit
ACTTTCAAGTTTCCCCACCCTTTCTCTGAGGCCCTGAGGTGTAGAACCAT32762.988314922418747No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT31062.833243635235845No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT25492.325157123701278No Hit
ACAAGTCAACAACCCCCACACCCCAACTTGCTCATTGTAGAACCATGTCG24042.192890437574685No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGGCCTTGAAGTTGTGC22632.0642724876171017No Hit
TGCAATTTGGTCTCTGCATGTACATAGCTGATGATATATAAACCCTATGT15231.3892562963503516No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT14471.3199303091391719No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA13571.237833745336459No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAGGGGGCTAGAA13171.2013463836463645No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA11591.0572213049704908No Hit
ACAAGTTGCAGGACTTTTTGCTGTACGGTAGCAGAGCTTAGGTGGCACCA9400.8574529997172229No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTGGAAC9330.8510677114214564No Hit
TTGCAAGGCTGATCATTTGCTGGTGTGTAACGTTCCTCTTGTAGAACCAT9090.8291752944073996No Hit
TTACTTTACCTTTCCAATTTGCTGAAGAACCACCTTCAACATTTAAGTGT9030.8237021901538855No Hit
ATGATCCATGGACATTTGACTGGTAGGTAAGTGGAGTGAAATTTAATGTA8890.8109316135623523No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT8510.7762686199567626No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG7710.7032938965765733No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC7200.6567725104217026No Hit
TAACATAAGAGATTCTGGGGCTTCAAGAGGTGTACAGGCATCAGGAGAGC6550.597480547675299No Hit
GGTCTCTGAGTCCTGCTGCTGGGAAGTAGCTTCCGTCAGGGCACTGATGG6330.577412498745747No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT6320.5765003147034946No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG6010.5482226093936713No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT5850.5336276647176335No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG5700.519944904083848No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG5580.5089986955768195No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT5490.5007890391965483No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG4590.4186924753938354No Hit
CAGCCAGACTGCCTTCCGTATACGGTAGCAGAGACCTGTGGGAAGCGAAA4390.4004487945487882No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA4370.3986244264642834No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA4280.39041477008401215No Hit
GGCACTCAGGAAAGTATCTCGTTCACTGACTGGACCCATGTAGAACCATG4220.38494166583049794No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT3670.3347715435066179No Hit
CTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG3480.317440046703823No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG3290.30010854990102803No Hit
CCAAAGCAGAAAGGGTAGTAGAACCATGTCGTCAGTGTACCCTAATCCAT3070.280040500971476No Hit
GGGAACTATTCCACATGGGTCAGTGTAGCAGCAAGTCATCTATCACCATG2910.26544555629543815No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC2910.26544555629543815No Hit
CCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG2740.249938427577148No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC2670.2435531392813814No Hit
CCACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT2600.23716785098561485No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT2530.23078256268984831No Hit
AAGAGGCTTCCAAATTGAGGGTTACGGTAGCAAATACAACAGCAAATCAA2530.23078256268984831No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT2310.2107145137602963No Hit
CCTTTCAAGTTTCCCCACCCTTTCTCTGAGGCCCTGAGGTGTAGAACCAT2260.20615359354903445No Hit
CCAAGTCAACAACCCCCACACCCCAACTTGCTCATTGTAGAACCATGTCG2220.20250485738002502No Hit
CGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC2210.20159267333777262No Hit
CAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT2130.1942952009997537No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT2130.1942952009997537No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT2070.18882209674623954No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG2060.18790991270398716No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT2040.18608554461948243No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT1910.17422715207020167No Hit
CAACTGTCAGTCTGCCATTCTTTAAGACAGCTAAGAGGGGAGGATCTAAC1880.1714905999434446No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGATTGAGTACAAACTGGTGGTG1870.17057841590119222No Hit
AGTCCTAGCCCTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGG1810.16510531164767805No Hit
GTAGTCAGTGCATTCTACAACAGCGCGCATGTTAGCAAGTTCATCAACCA1780.16236875952092095No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCACT1720.15689565526740676No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT1650.1505103669716402No Hit
CTTAGTTTCTTATCATTTTGGCTTCTATACCTCCATGATCTCCAACAAAG1510.13773979038010709No Hit
CTGTGACAGTTCCCAGCGGGTCCGGATATGAATGATAGACTGGACAATGG1260.11493518932379797No Hit
ACAAGTCAACAACCCCCACACCTGAATCCTTAGCCTCTACTGTGTAGAAC1230.11219863719704089No Hit
AACGTGGCCATCACAATAGATGTAGAACCATGTCGTCAGTGTAGATCTCG1220.1112864531547885No Hit
TTAAATGGAGTCATCTGAGGAGAATTCATGTAGAACCATGTCGTCAGTGT1180.10763771698577905No Hit
CAAGGAGAATGATTTGTAGTGGCCAAAATTGAATGCTATGCTTAGATGTA1150.10490116485902196No Hit
CGCAATTTGGTCTCTGCATGTACATAGCTGATGATATATAAACCCTATGT1120.10216461273226488No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)