FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008101656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008101656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20710643
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGC815900.3939520371241009No Hit
GGCTCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGC786650.37982886383585485No Hit
GGGGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATA585520.28271454440115645No Hit
GGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCG568600.2745448318528787TruSeq Adapter, Index 27 (97% over 39bp)
GGCGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATA419590.20259631726547556No Hit
GGCCCCCCACCGCGACGCGGCGCGTGCGCGGGCGGGGCCCGCGTGCCCGTT307930.1486820085692173No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTG288120.13911687821570773No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG277050.13377180032507924No Hit
GGGCACCGCGACGCGGCGCGTGCGCGGGCGGGGCCCGCGTGCCCGTTCGTT230440.11126646333481775No Hit
CGGGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATA225590.10892467220839064No Hit
GGGGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCC224200.10825351969999193No Hit
GGCCCCCACCGCGACGCGGCGCGTGCGCGGGCGGGGCCCGCGTGCCCGTTC217690.10511020831173615No Hit
GGGAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAAACTTTAAAT210370.10157579366319046No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)