FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008101675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008101675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20378843
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGC648380.318163302990263No Hit
GGGTCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGC536590.263307391886772No Hit
GGGGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATA284670.13968899019438935No Hit
GGGCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCT281470.13811873421862075No Hit
GGCCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCG261280.12821140042150578No Hit
GGCCCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCC259000.12709259303877066No Hit
GGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCG256430.1258314812082315TruSeq Adapter, Index 3 (97% over 36bp)
GGCGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATA235800.11570823721444834No Hit
GGCCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGAC223250.10954988955948086No Hit
GGGCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACC214620.10531510547482995No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)