FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008101685

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008101685
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37395910
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATA4172641.1158011664912018No Hit
GGCGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATA3375550.9026521884345107No Hit
GGCCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGAC2574830.688532515989048No Hit
GGCCCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCC2267430.6063310132043852No Hit
GGGACCGCGACGCGGCGCGTGCGCGGGCGGGGCCCGCGTGCCCGTTCGTTC2127300.5688590008907392No Hit
GGCCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCG1882650.5034374079946176No Hit
CGGGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATA1534060.410221331691086No Hit
GGGCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCT1337970.35778511607285396No Hit
GGGAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTAT1215060.3249178854051152No Hit
GGGCACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGA1087350.2907670919092489No Hit
GGCAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGC1050510.28091574720337065No Hit
GGGCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACAC1048730.2804397593212734No Hit
GGCATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATAC1028240.27496055049870427No Hit
GGGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCC1021070.2730432285241889No Hit
GGCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCT981680.26250999106586786No Hit
GGCAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTAT981340.2624190720322089No Hit
GGCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGT905820.24222435020300348No Hit
GGGCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACC842500.22529201722862208No Hit
GGGGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTA821790.21975397844309713No Hit
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG798510.21352869872667893No Hit
GGCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACT788260.21078775727078175No Hit
GGGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGC785050.2099293746294715No Hit
GGGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATAC771770.20637818413831885No Hit
GGGCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCT751290.20090164940497504No Hit
GGGCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAG748320.20010744490507118No Hit
GGCCCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTA731120.19550801143761445No Hit
CGCGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATA695780.18605778011552598No Hit
GGCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCT686780.18365109981278702No Hit
GCCGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATA686180.1834906544592711No Hit
GGCCTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTG676080.1807898243417529No Hit
GGCAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTA660260.17655941518738277No Hit
CGCCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGAC642840.1719011517569702No Hit
GGCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTC633950.16952388643570915No Hit
GGCCCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACA627630.16783386204534131No Hit
GGGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTG620060.1658095765018153No Hit
GCGGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATA618910.16550205624090977No Hit
GGCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACAC608940.16283598928332002No Hit
GGGAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTA604850.16174228679018643No Hit
GGGTAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCC593610.15873661050098795No Hit
CGGCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCT586710.1568914889355547No Hit
GGCCACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGA581930.1556132742858778No Hit
GGGCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACT568680.15207010606240096No Hit
GGGCTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTG567730.15181606758600072No Hit
GCCCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGAC543530.1453447716608581No Hit
GGGCAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTT536120.14336327154493633No Hit
GGGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGC536060.14334722700958474No Hit
GGCCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCA525350.14048327744932534No Hit
GGGGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCC496580.13278992274823637No Hit
CGCCCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCC490050.13104374248413797No Hit
GGCCCCCCACCGCGACGCGGCGCGTGCGCGGGCGGGGCCCGCGTGCCCGTT486770.1301666412182509No Hit
GGCCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGC479300.1281690965669775No Hit
CGGAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTAT477680.12773589411248448No Hit
CCGGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATA477050.12756742649129277No Hit
GGGGGAGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTC476180.12733478072869467No Hit
GGCCCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGG467910.12512330893940005No Hit
CGGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCC460750.12320866105410994No Hit
GGCCCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAAT454610.12156677026979687No Hit
GGGCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATCCCA453030.12114426417220493No Hit
CGGCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACC444680.1189113996691082No Hit
CGCCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCG442810.11841134498398354No Hit
GGGGGTTAAAAAAAGTAAAAGGAACTCGGCAAATCTTACCCCGCCTGTTTA440850.1178872234958315No Hit
GGGGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGG438110.11715452304810874No Hit
GGCTCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGA435120.11635497036975434No Hit
GGGCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTC426920.11416221720503658No Hit
GGCAGCTCAACACCCACTACCTAAAAAATCCCAAACATATAACTGAACTCC422520.11298561794591976No Hit
GGGCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGT418620.11194272314806619No Hit
GGCCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCC408370.109201781692169No Hit
GGGCCTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGC406650.10874183834542334No Hit
GGCCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAG406460.1086910306501433No Hit
GGGCAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACC398310.10651164793155188No Hit
GGGCACCAATTAAGAAAGCGTTCAAGCTCAACACCCACTACCTAAAAAATC398060.10644479570092025No Hit
GGGGTCTCGTCGCGCGCGCGTCCGCTGGGGGCGGGGAGCGGTCGGGCGGCG390480.10441783606816896No Hit
CGGCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACAC389080.10404346357663179No Hit
GGGAGTCCAAAGAGGAACAGCTCTTTGGACACTAGGAAAAAACCTTGTAGA386770.10342574896559542No Hit
GGGAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAG385720.10314496959694255No Hit
GGGGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAA381270.10195499989169939No Hit
CGGCACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGA379140.10138541888671782No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)