FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008101691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008101691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32293455
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATA4200791.3008177663244767No Hit
GGCGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATA2973540.9207871997592081No Hit
CGGGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATA1587030.4914401385667777No Hit
GGGAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTAT1128940.3495878654049249No Hit
GGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCG1031630.31945482451475077TruSeq Adapter, Index 6 (97% over 36bp)
GGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG985220.3050834913761937No Hit
GGCAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTAT753660.23337855921579156No Hit
GCCGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATA680970.21086935417718544No Hit
GGCATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATAC628230.19453787152845678No Hit
CGCGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATA618700.1915868091537434No Hit
GGGGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCC605880.187616964490173No Hit
GGGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATAC598370.1852914158612016No Hit
GGCCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGAC596220.1846256462803376No Hit
GGCCCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCC586910.18174270916506147No Hit
GGGAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTA585830.18140827607327864No Hit
GGGGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGGCTCGC568460.17602947718043796No Hit
GGCAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTA564660.17485276815379464No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTG536170.1660305470566714No Hit
GGGACCGCGACGCGGCGCGTGCGCGGGCGGGGCCCGCGTGCCCGTTCGTTC526810.16313212692788678No Hit
GGGAGAGGTCTTGGGGCCGAAACGATCTCAACCTATTCTCAAACTTTAAAT524530.1624261015119008No Hit
GGGTCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGC484960.15017284462130173No Hit
GCGGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATA469920.14551555415795553No Hit
GGCCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCG468880.14519350747697946No Hit
GGGCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCT457570.14169124982136475No Hit
CGGAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTAT447230.1384893626278142No Hit
GGCTCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGC441900.13683887338781187No Hit
CCGGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATA420700.13027407566022278No Hit
GGGGGATGTGAGGGCGATCTGGCTGCGACATCTGTCACCCCATTGATCGCC406550.12589238283732723No Hit
GGCAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGC392550.12155713905495712No Hit
GGGAAAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCT377950.11703609911048539No Hit
GGGGAAACGATCTCAACCTATTCTCAAACTTTAAATGGGTAAGAAGCCCGG373200.11556521282718123No Hit
GGGCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACC372290.11528342198132717No Hit
GGCCCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGG335270.10381979877965984No Hit
GGGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGC331850.10276076065568085No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)