FastQCFastQC Report
Wed 8 May 2024
EGAF00008101711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008101711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24015714
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTC2332970.9714347864069334TruSeq Adapter, Index 1 (97% over 36bp)
GGGTCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGG1003590.41788888725107237No Hit
GGCTCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGG856140.35649158713332446No Hit
GGCCCCTTCCCGTGGATCGCCCCAGCTGCGGCGGGCGTCGCGGCCGCCCC559640.2330307564455506No Hit
GGGCGTCGCGCGCGCGTCCGCTGGGGGCGGGGAGCGGTCGGGCGGCGGCG530780.22101362466258548No Hit
GGGGCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCT395690.16476295478868544No Hit
GGGGGGGGCCCTCCGTCCCCCTAAGCGCAGACCCGGCGGCGTCCGCCCTC387320.16127773673520596No Hit
GGGCACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCG345690.14394325315499676No Hit
GGCCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGA340560.1418071517673803No Hit
GGGCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTC325690.13561537250152128No Hit
GGCAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCT298450.1242727990514877TruSeq Adapter, Index 1 (97% over 36bp)
GGGCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACA265000.11034441865854998No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)