FastQCFastQC Report
Fri 4 Aug 2023
EGAF00008101712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008101712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31174057
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCG2198630.7052755436996859TruSeq Adapter, Index 6 (97% over 36bp)
GGGCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCT1237220.39687487579816766No Hit
GGGGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATA1154470.3703303679723175No Hit
GGGCACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGA1037730.33288256321594584No Hit
GGCCCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCC843110.27045244704595234No Hit
GGCCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGAC837890.2687779777909561No Hit
GGCGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATA821880.2636422971831995No Hit
GGGCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACAC807480.2590230716521754No Hit
GGCCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCG804910.2581986682067079No Hit
GGGTCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGC668430.21441867511822413No Hit
GGCCCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTA643310.20636069280299318No Hit
GGGGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTA641920.20591480922742908No Hit
GGCTCTGGCTGCGACATCTGTCACCCCATTGATCGCCAGGGTTGATTCGGC596450.191328963054119No Hit
GGCCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGT578360.18552606098077No Hit
GGGAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAA567170.18193653780770339No Hit
GGGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCGAGCC556320.17845607968189703No Hit
GGGCAGTGACACATGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAG517230.16591680704247125No Hit
GGGCAATATCTACAATCAACCAACAAGTCATTATTACCCTCACTGTCAACC497430.15956537193731316No Hit
GGCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCT488710.15676817425463743No Hit
GGGCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAAT487100.1562517191779049No Hit
GGCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACT481260.154378366601434No Hit
CGGCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCT450100.14438287579957912No Hit
GGGCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGT443100.1421374189442202No Hit
GGGCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACACT436550.14003631288670576No Hit
GGGCCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTA423960.13599769834256736No Hit
GGGCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTC423840.13595920479647547No Hit
GGGGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTT411710.13206814884568924No Hit
GGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAAT404190.1296558866239322No Hit
GGCCCGAAACCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTC395660.12691963705590198No Hit
GGGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAG395460.12685548114574885No Hit
CGGGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTATA387450.1242860369441167No Hit
GGGCTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTG376650.12082161779584866No Hit
CGGCACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGA368720.11827783595827773No Hit
GGCCACCCGTCTATGTAGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGA360960.115788586644337No Hit
GGGCAGACGAGCTACCTAAGAACAGCTAAAAGAGCACACCCGTCTATGTAG359460.11530741731818864No Hit
GGCCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAG353700.113459727105779No Hit
GGCCTACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTG353360.11335066205851872No Hit
GGGAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAA346910.11128163395608084No Hit
GGGCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTT344010.11035137325886071No Hit
GGGAGATGAAAAATTATAACCAAGCATAATATAGCAAGGACTAACCCCTAT332510.10666240842505677No Hit
GGGCGGAGGGCGAGGCCCGCGGCGGCGCCGCCGCCGCCGCGCGCTTCCCTC332140.1065437199912735No Hit
GGCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTTGGACAC322460.10343857393986287No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)