FastQCFastQC Report
Mon 10 Jul 2023
EGAF00008101916

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008101916
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123274018
Sequences flagged as poor quality0
Sequence length90
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA89622017.2701459280738305No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT65478695.311637526084369No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT41393173.357817865561906No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTC30141322.445066729308685No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG22674891.8393892214984018No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT20998151.7033719141043977No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTC15025521.218871603584788No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCT14090241.1430016015215794No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC9676630.7849691408614587No Hit
ACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAG7372370.5980473517136433No Hit
GCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGG5907640.47922831557254836No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC4784230.3880971901151141No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT4458650.3616861097202169No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT3699200.30007945388784196No Hit
TGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCC2795270.22675256678986486No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT2598600.21079867778788552No Hit
GTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTG2430850.1971907819212967No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGCGTGGTCAT2366450.19196664783003992No Hit
GTCTCAGGGACCCCCCAGGCTGGATCAAGCCTCCTCCAGACTCCACCAGC1959050.15891832129621994No Hit
CCCAGCTCATGTAGTTGCTACTGACGGTGAACCCAGAGGCTGCACAGGAG1934890.1569584598110528No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1739370.14109785891784593Clontech SMART CDS Primer II A (100% over 26bp)
GCCTCCTCCAGACTCCACCAGCTGCACCTCACACTGGACACCTTTTAAAA1706180.1384054829785787No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA1673080.13572040784782402No Hit
CTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCAC1665210.13508199270344218No Hit
GCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTCCA1648740.13374594474563165No Hit
CCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATGTAGTTG1648230.13370457349739343No Hit
CTCAGGGACCCCCCAGGCTGGATCAAGCCTCCTCCAGACTCCACCAGCTG1521620.12343395832202046No Hit
CTCCAGACTCCACCAGCTGCACCTCACACTGGACACCTTTTAAAATAGCA1498610.12156738494562577No Hit
CCCAGAGGCTGCACAGGAGAGTCTCAGGGACCCCCCAGGCTGGATCAAGC1456680.11816601937968793No Hit
TAGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGC1440190.1168283490199857No Hit
GGAGAGTCTCAGGGACCCCCCAGGCTGGATCAAGCCTCCTCCAGACTCCA1423830.11550122427257947No Hit
CCCCAGGCTGGATCAAGCCTCCTCCAGACTCCACCAGCTGCACCTCACAC1421860.11534141768624756No Hit
GCTATAAATAACTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGA1365820.11079544758571915No Hit
CTGAGACCCACTCCAGCCCCTTCCCTGGAGCCTGGCGGACCCAGCTCATG1330800.10795462187336183No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)