FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008101978

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008101978
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86781751
Sequences flagged as poor quality0
Sequence length90
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT56207126.476836356989386No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA37822444.358340269027298No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGT13290431.531477510749927No Hit
CGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGG9270571.0682626120323384No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCTC6575040.757652377859949No Hit
GGTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTT4667280.537818141051337No Hit
GGGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACT3565530.41086172598660753No Hit
TAGCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGC3442800.39671935174481554No Hit
GTGGTACCCAGTTATCAAGCATGCCAGGACCACAGGGCTGTTATCCTTTG3247740.37424227589046916No Hit
GCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTCT3196350.36832052397744314No Hit
AGCTGCTGGCCGCGTACTTGTTGTTGCTTTGTTTGGAGGGTGTGGTGGTC3191220.3677293858705386No Hit
ACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAG2932030.33786250752188673No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2625430.30253249902735885Clontech SMART CDS Primer II A (100% over 26bp)
GCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAGGG2589150.2983518965871062No Hit
GTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCA2381690.27444594889540774No Hit
TAGCTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGT2257200.2601007670379917No Hit
CCTGAGGACTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGC2146440.24733771504564364No Hit
CTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCTCG1931860.22261131836346562No Hit
CGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTC1577060.18172714675923052No Hit
TCCTGAGGACTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAG1478420.17036070175629436No Hit
CCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGGC1291760.14885157134015423No Hit
GGAAGTTTCTGGCGGTCACGCCCTGTCCGCTTTCGCTCCAGGTCACACTG1146090.132065784199261No Hit
CTGCTGGCCGCGTACTTGTTGTTGCTCTGTTTGGAGGGTTTGGTGGTCTC1135140.13080399818159927No Hit
TCCTGAGGACTGTAGGACAGCCGGGAAGGTGTGCACGCCGCTGGTCAGGG907370.10455769669823786No Hit
CTGAGGACTGTAGGACAGCTGGGAAGGTGTGCACGCCGCTGGTCAGAGCG881090.10152941025584976No Hit
GCTGCTGGCCGCGATCTCCTCCACCTTCATGATCTGAATGGAGTGGGCTC875690.1009071596170029No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)