FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434437
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCTCTGAGTCCTGCTGCTGGGAAGTAGCTTCCGTCAGGGCACTGATGG158253.6426455389389023No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT147653.398651588147419No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT122202.812835923275412No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG113892.6215538731737857No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT104412.403340415296119No Hit
GTAGTCAGTGCATTCTACAACAGCGCGCATGTTAGCAAGTTCATCAACCA102662.3630583951182795No Hit
TAACATAAGAGATTCTGGGGCTTCAAGAGGTGTACAGGCATCAGGAGAGC94802.1821345787766693No Hit
CAGCTGTGCCATTTGAGGGATGCTCAGGTCGGTTTGGCTCTGCAGCAGGT92692.1335659715908175No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAGGGGGCTAGAA85981.979113197080359No Hit
CAACTGTCAGTCTGCCATTCTTTAAGACAGCTAAGAGGGGAGGATCTAAC76601.76320156892714No Hit
CTTAGTTTCTTATCATTTTGGCTTCTATACCTCCATGATCTCCAACAAAG76321.756756445698686No Hit
CAATGATGAACACTGCGGGCTTCCAGACAATGTTAACACGCTGAATGCCA73651.6952975920559252No Hit
GCACAAAGGCCCCATTCTGGCACTGACCTATTGCATCCCCAGCCCCAGAC68821.5841192163650886No Hit
GCTGTCTACCTGACCAATCGATGGGGCATTCATTATGACATGAAGATCAG59571.371199966853652No Hit
CCTGGAGTCGATTGATTAGAGCCTAGTCCAGGAGAATGAATTGACACTAA56881.3092807472660017No Hit
TGCAATTTGGTCTCTGCATGTACCACAGAATTGCTGCTTCCCTCAGCCTT56531.3012243432304338No Hit
CTGTGACAGTTCCCAGCGGGTCCGGATATGAATGATAGACTGGACAATGG54951.264855433584156No Hit
CAGAAATGACATCTACCTGTTACTCACAGAAACCACACACCAAAGGAACA52991.219739570984976No Hit
GGATCCGTGGCGAGTTCGTCCACTGTTACAGTGGCCAGTGGTAGGGGAGA49101.1301983947039502No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG47561.0947502169474514No Hit
TAGGAGCCAGGAGAGAATGACCTGTCCCGGTAACTGGAACGGAAGGCAAT46201.0634453326949593No Hit
AAAGAGAGTGAGTCGTTGCGAAGCCCTGTGTTTCAGCTCATTGTGATTAA45781.0537776478522778No Hit
GGAAGGTGACGTGAAAGATCACTGTGCAGCAGCAATCTTGACTTCTGGAA45671.051245635155385No Hit
GGTTGCTGAGGTGGTGTGATTGCCTTTGGTTCTGGAGAGTCTGGGGGAGA45471.046641975706489No Hit
CGACAAGCAACACATCATCGTCCGACTCCAGCTGAGCCTCCGCTGCTGCT44671.0282273379109053No Hit
CTGCCCCCTTACCCTCCACACCCCCAGGATTCTTACAGAAAACAAGTGGT43481.0008355641899747No Hit
AGTCCTAGCCCTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGG41740.9607837269845801No Hit
AGGGCCTATTAGCCATATCTCCCAGCTCTCAACAGGAGGTGGAGAGGCAG41500.9552593356459049No Hit
AACAGATATGCTATAGTACAGAAGGCAAAAGAAAAGTGATTACCTTGCTT38030.8753858442075605No Hit
GTGTACACAGTGTCCACCAAGGTCCTGAGATCCTCATTTCTCTTCCTTGT36990.8514468150733017No Hit
ACTTTCAAGTTTCCCCACCCTTTACACAGTTTCCCCGCGGAAAACGCCGC36920.8498355342661882No Hit
GGTCTTTGTGCCCAGACTTCAGCTCCCGTTCTTTCCTGGTCTTCTCCTTG36600.8424696791479548No Hit
CACTGGGCAATTGGTAGGGTAAATCCACAGGGCGCCCGCGGAGGTGCAGA36300.8355641899746108No Hit
TGGTGGCAAACTCTCCTTCTCAACCAAAAAGAAAGCAATCAACTATTTCA35840.8249757732421502No Hit
TCCACCAATCTCTCAAACCGATCAGCCAATACTGTTTCTGCAAGTGTGTC32720.7531586858393737No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTGCTTGATTTTCTTACTTGGTCCAA32410.746023013693585No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA31610.7276083758980013No Hit
GGCACTTTTCTTCCCCGCTTACTCTAATCACTTACCCACTGAGAACAAGG30460.7011373340668497No Hit
ACCTGTATAGGGTATGCTCTTTGAATAATTACATCAACACAACCAACATT28130.6475047014872122No Hit
CACTCAAGAGCACATGTACAGCTGTACCTGATCAGTTCTAGCTGAGCCAG27440.6316220763885212No Hit
GTTGTAAAATTTCTTCACTCTGAATGTCAGCAAAAACCTTATGTGAATGC27350.6295504296365181No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC27260.627478782884515No Hit
AAGAGGCTTCCAAATTGAGGGTGGGCGCCTGGGAGGGGTGTTTCTGTTGC25150.5789101756986629No Hit
AGGAAATAATGAGCGAACCCCACTTCCCCACAACGGCACCCCACATGAAA25050.5766083459742148No Hit
GCAGTCTGGGGCCCTTTGGCAGGCCCTGGTTGCCCTGAGGCGGGTGCTGC24690.5683217589662022No Hit
GGTTGAACTAAAACGACAATACAATGACCACCATTCAAAATTGAGAGGTC24030.5531296827848458No Hit
TGCCCTGACTTTCAACTCTGTCTCCTTCCTCTTCCTACAGTACTCCCCTG23530.5416205341626058No Hit
AGCTTCATCATACCTTTCACTAAGACAAGAATTTAGAAGTGGACAGGAAA23440.5395488874106027No Hit
ATCTCCGCAAGAAAGGGGAGCCTCACCACGAGCTGCCCCCAGGGAGCACT22830.5255077260914701No Hit
CCTGGTCCTCTGACTGCTCTTTTCACCCATCTACAGTCCCCCTTGCCGTC21060.4847653399687411No Hit
CATGAGGGTAGTGTTGACAAAGATTAAAACAGAAAATGCTTTATGTTTAG20420.47003362973227425No Hit
CTGAGGTCGCTCAGTTCCGGAGCTGCAACCCGGAGTCCCAGATGATCGGG20130.4633583235313751No Hit
GGCATTCCTTTTTGAACAGTACCCATTCCCTTGATGTCTACAATATCACC19660.4525397238264697No Hit
TAATCTATATTGATCAGGTGAAGTAAAATGGAGAAAGGAACTGGTAAACA19600.4511586259918009No Hit
TGTGGTTTAGCCCTCGAAGTAAGAAAGTCAGATATGTTGTGAGTAAAGCT19310.4444833197909018No Hit
CCTCCTGGCCCCTGTCATCTTCTGTCCCTTCCCAGAAAACCTACCAGGGC18650.42929124360954524No Hit
TCTAAAGTATGCAGGTTTTGTTCAAGAAGTGCGGTACCTGCTGAATACAA18640.4290610606371004No Hit
AAGACCCAGGTCCAGATGAAGCTCCCAGAATGCCAGAGGCTGCTCCCCGC17660.40650312933751037No Hit
ATCACAATATCTCATTTTGCCCTCTTTGCAACCAGTGCACATTACTGGGT17470.40212965286105923No Hit
GACAGGTTCATGAGTGCTTCCCCCACATTCCTTCAGGTGACTGGTTCTTC17410.40074855502639045No Hit
AGGGATGCAAAGGCCTCAGCTGTTTGGCTAAGAGCAGCCACCAAGGCTCA17040.39223178504593303No Hit
GCAGAATTCCGAAGTTCAGCTGCATGCTGGTTTCCTTCACGACAGGTGTG16790.3864772107348131No Hit
CCTCAGCTTGCCATCAGGAGTCGTTACCTTTGCTAAGCTCGGAGGGTTTC16400.37750007480946607No Hit
CCAACTGGCCAAGACCTGCCCTGTGCAGCTGTGGGTTGATTCCACACCCC16170.3722058664432357No Hit
GTCACTGACAACTGGCTTGTGCAACATTTTGACATGGAAGTCACAGACTA15850.36484001132500227No Hit
ACTAGATATTCCTTGTCATTATCTGCACGCTCTATACTGCAAATGCTATC15230.3505686670334249No Hit
TTATCGCCATCCTTCTGAACTTAAGAAGAGCTCTAAAAAGCCAGTGTACA15080.34711592244675293No Hit
AGTGAGGATGAAGAGCTTCCCTGCTTCCAACACTTGTTATTTGGTAAAGT14490.33353512707250993No Hit
CTGTGTCACCAAGGGTTTCGTTGGGGCCATGAAGACCACCTTTCCTGAAG13220.30430188957202076No Hit
GTGTCAATGGCACTGAACCCTGTTTCAGGCCCATCAGTGTTTCCATAAGT12870.29624548553645297No Hit
CCAGATCCTTAACATTGGTCCGTATTTGAAGTCCCACTTTCTCATGGTTA12450.2865778006937715No Hit
ATTAGGATGTCTGGCACATGCACAGGACAACCAAGTTCAAGAACCTCTCA11980.275759200988866No Hit
CTACCAGTGCCAGGAGCTGGACACCTACCTGATACCCCAGATCCCCCACA11890.27368755423686286No Hit
CAGTACTTGTCCCTGAGGTAGTTTCTTTTCGAGAAGTTTTGGATGGAGGT11390.2621784056146231No Hit
GTTCAAATGAGTAGACACAGCTTGAGAGAGAGAGAGAGACAGAGATTTCA11100.25550309941372396No Hit
CACAACCTTGCACTGCTTTATGAAGTGTTTCACCAAACATATTTCGAAGT10950.252050354827052No Hit
AAAAGCCCCTAAACCCCACTTCATTTTCATCTGTTAAATAACCAGAAGCA10910.2511296229372728No Hit
TTCTCTTCCGCACCCAGCAGTTTGGCCAGCCCAAAATCTGTGATCTTGAC10710.24652596348837694No Hit
TATGAGAACACGCAGAGGGAACTTGGCCTCCTACTGCTGCTTTCAGAGAG10560.24307321890170494No Hit
TACTGGTGTGGGTTATAGAAGAAACATGAGAGATTTAGTTCAAAAGGAAG10530.24238266998437055No Hit
CTTGGGCCTGTGTTATCTCCTAGGTTGGCTCTGACTGTACCACCATCCAC9790.22534913002345566No Hit
ACACCGGATATCTGCTTCCTCACAGAGGTGGCGGAAACAGGACATGGCAA9440.2172927259878878No Hit
TTACCCACAGCTCCAAACATCTATGGCTGGTGTGTAACGTTCCTCTCCTA9440.2172927259878878No Hit
GCTGCCCCCACCATGAGCGCTGCTCAGATAGCGATGGTGAGCAGCTGGGG9310.21430034734610542No Hit
GATGTTTTGAGATTTTCAGTTTGTCTACATAAATTATCACTTAAGAGCTT9200.21176833464921266No Hit
ATGTTACCTTAAGATCAACCACCATGGACTGAACAAGTAGGAAAATGACA9100.2094665049247647No Hit
GTAGACGATGTAAAGCAAGCACATTGCCGTCACTTATGAAGGAAAGACTA8870.20417229655853436No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCTACCCAATT8790.20233083277897598No Hit
GTGGATTAACTGACGAAGGATTTTGATTTCACGAATGGCTGTGATTGGGA8760.20164028386164162No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT8670.19956863710963846No Hit
AATGCAGATGATTACCTCGCTCTGGCAAATGATTTCATTAAAGATTCAGA8580.19749699035763527No Hit
CCACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT8500.1956555265780769No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA8450.19450461171585293No Hit
GGCTCCTTATCTCCCCTCCCCGTATCTCCCTTCCCTGATTACCTTTGCGA8260.1901311352394018No Hit
CCACACAGCAAAGCAGAAACTCACATCGAGGATTTCCTTGTTGGCTTTCG8260.1901311352394018No Hit
CACGCACACACAAGCAGATTGCTCAGATTACTAGAGAGCTCCGGAGGACG8220.1892104033496226No Hit
AACATGTTCCAGAGCAGTTGGTAGAGTAAATGCCGAGAATCAAACAGAGT8210.18898022037717782No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG8080.18598784173539548No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT8070.18575765876295067No Hit
TTATTTTTTGGGGGGAAATTTTTTAGGATCTGATTCTTCTGAAGATACCG8010.1843765609282819No Hit
CGACTCAGAGACCGCTTGCTCAGGAAAGGGCTGCTGGACCGCCTTCGACC7840.18046345039672035No Hit
TCAGATTGAGACCTCCTTTCCACCACCATGCGATTACTCACGATGTAATC7180.16527137421536378No Hit
GCAGTCTGGGGCCCTTTGGCAGGCCTTGGTTGCCCTGAGGCGGGTGCTGC7140.1643506423255846No Hit
GAGTCCTCCTTCTGTGAGCAAACTGGCACCCTGTGAGCAAGACTGGCACC6440.14823783425444886No Hit
GGAGAGCTGTAAATTCTGGCTTATAAGGTGTTCATACATACCCCATTTCT6090.140181430218881No Hit
ACAAGTTGCAGGACTTTTTGCTGTTTCTTTTTCTACATTTTCATGTACTT5950.13695886860465384No Hit
TCTGAGCACCAGTGGAGATTTTGTTAAGCAAACGGTGCCCTCAGAAAATA5930.13649850265976424No Hit
CCCCCTCCTCTGTTGCTGCAGATCCGTGGGCGTGAGCGCTTCGAGATGTT5690.13097411132108913No Hit
ATGAGGTACTCGTCGGCATCCACCACGTCGTCCATGTCTTCTTCATCCAT5610.12913264754153075No Hit
GCTGACCTAAAGCCACCTCCTTACTTTGCCTCCTTCTGCATGGTATTCTT5510.1268308178170828No Hit
GCACCTGAGCTCAATGAGATTCATGACCGGTCTGTTTACCTGCTGCCTCC5320.12245734134063166No Hit
GTCCATGCTCTTGAAAATAATCATCTACTAACCTTTTTTATAAAACAGTG5150.11854423080907013No Hit
AGAAAGAAATATATGGTAAGTTTCAAGAATACATCATACCTGTATAGGGT5090.11716313297440135No Hit
GGCATTCCTTTTTGAACAGTACCCGTTCCCTTGATGTCTACAATATCACC5010.11532166919484299No Hit
AGTTGAAGAAGCACCCTTTCTGGGCTTAGGCATCTATTAGCAAATTCCTT4790.11025764380105744No Hit
AATTACCTCCAGTTGCTCCTTCTGGGTAAAACCCTTCGTGCTTTTCCCTG4640.10680489921438552No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG4550.10473325246238235No Hit
CGTCAGCTATTGAATTACTTTCCAAAAGAGAAATTTCATTGAATTTTTTA4420.1017408738206No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)