FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93213
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC990210.62298177292867No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG977410.485661871198223No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC90529.71109180049993No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTA79018.47628549665819No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC51385.512106680398657No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT50495.416626436226707No Hit
TAAAGGAACTGTAGTCGCCCTGAGACTTGGTCTCCGCAGTCTATATCTAT29793.1959061504296606No Hit
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT18251.9578814113911152No Hit
TCAAATCATCCATTGCTTGGTACGGTAGCAGAAAGTCAGTCCCATGGAGA17511.8784933432032012No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT16681.7894499694248656No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA15061.615654468797271No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTACGGAAGGCAGTCTGGCTGAG14981.6070719749391178No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTT13431.4407861564374067No Hit
CTGGCTGAGCACATAGAGCACACAGCTCAGAATACTAGTAGACCAAGTCT12461.3367234184073036No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA12131.3013206312424233No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA9911.0631564266786822No Hit
GGAGTTGAACCCAGCCGTCAGTGGGGTTCAGTGCCATTGACACAGACCAA9421.010588651797496No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA9411.009515840065227No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC9290.9966420992779976No Hit
AGGACATGGCCGCGCAGACGGATGTGTTTGTGCGGCGGGGTGGGAGGGAT9210.9880596054198448No Hit
GGGGACGCTCTTGTATTATCTGTACACTGACTTATGGTATCAAATAATAA8790.9430015126645425No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTACTA8340.8947249847124328No Hit
TGGGTCCAGTCAGTGAACGAGATACTTTCCTGAGTGCCAGACCAAGTCTC8140.8732687500670508No Hit
GCAGAGCCAAACCGACCTGAGCATCCCTCAAATGGCACAGCTGCTTAACA8130.8721959383347817No Hit
GTAACTTGATTTGCTGTTGTATTTGCTACCGTAACCCTCAATTTGGAAGC6220.6672888974713828No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTACTAATCG5400.5793183354253162No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC4840.5192408784182464No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTACT4830.5181680666859773No Hit
GGATGAGAACTCCGAGAAGCAGCATCTTTCCAGTTCGTATAAATAAGACC4720.5063671376310172No Hit
TCCTCCCCTCTTAGCTGTCTTAAAGAATGGCAGACTGACAGTTGGTCAGA3700.39694034093956854No Hit
TCTCCACTTCACCCCGTCAGTGTAACCAGTTCACCTTAACCATTGCAGAC3690.39586752920729945No Hit
GGGATAAAAGCACAAAGGTCTGCTACCGTAACCTTCAGAGCTAGGGAAAA3360.36046474204241896No Hit
AACTTGGGCATGGAGCATGTATGTGTGTGTGTGTGTGTGTGTGTGTGTGT3290.35295505991653525No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC3190.3422269425938442No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTG3180.3411541308615751No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA3110.3336444487356914No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCGAGACCAAGT2970.3186250844839239No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTACTAATC2940.3154066492871166No Hit
CTCATTCTTTCCTTGATTTTCTTCCTTACGGTAGCAAATACTGAGCATCA2920.3132610258225784No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTACTA2910.3121882140903093No Hit
AAGGGAAAAGGATGGATTAGGGTGAGGCATAACTGCACCCTTAGACCAAG2460.2639116861381996No Hit
AAATGTCCCAAAGCTAAATCCAGAAGACCAATCACCACTGACTCTGATCC2420.2596204392091232No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG2310.24781951015416304No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAC2310.24781951015416304No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTACTAA2270.2435282632250866No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC2260.24245545149281755No Hit
TCATGCTGTTTACATTCACTAAGGCTACGGTAGCAGAGTCACAGTCACAG2230.2392370162960102No Hit
TCTATGCTTGTTTCCCGACTGTGTGTGGCAGAGCAAAGGGCCCCAGACTG2160.23172733417012648No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTACTAA1950.2091982877924753No Hit
CTGATGTTGAATTAACCAAAAATATTCCCTCTGCGTGTTCTCATAAGACC1840.19739735873751516No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTACTAATC1570.16843144196624935No Hit
CACCATTTATCTGAGGACTGGATACGGTAGCAGAAGCCTTTTTAGCCCTA1530.16414019503717292No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT1470.1577033246435583No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT1300.13946552519498354No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTACTAAT1230.1319558430690998No Hit
AAGTCCTCCATTTCTGTATCCATGGAGGAAGTAGGCAGCTGAGACCAAGT1230.1319558430690998No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTACTAAT1130.12122772574640878No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACGAGA1080.11586366708506324No Hit
GAAGTATTACAAATGGAAGATGATCTGGTGATGGGTGCACTTTTCTGAGG1000.10728117322691041No Hit
GGTCTCTCATGGCACTGTACTCTTCTTGTCCAGCTGTATCCAGTATGTCC1000.10728117322691041No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG950.10191711456556489No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)