FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93213
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT967410.378380697971314No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG92159.885960112859795No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG90879.748640211129349No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT77988.365785888234473No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA51075.478849516698315No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC50165.381223649061826No Hit
AGTTATAGATATAGACTGCGGAGACCAAGTCTCAGGGCGACTACAGTTCC27362.9352128994882687No Hit
CCATGGGACTGACTTTCTGCTACCGTACCAAGCAATGGATGATTTGATGT17431.8699108493450485No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT16601.7808674755667129No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT16291.7476103118663706No Hit
CAGCCAGACTGCCTTCCGTACGGTAGCAGAGACCTGTGGGAAGCGAAATG14911.5995622928132343No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT14891.597416669348696No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAGGGGGCTAGAA12811.3742718290367224No Hit
ACTAGTATTCTGAGCTGTGTGCTCTATGTGCTCAGCCAGTGTAGAACCAT12461.3367234184073036No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT11831.2691362792743501No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT9881.059937991481875No Hit
GTGTCAATGGCACTGAACCCCACTGACGGCTGGGTTCAACTCCTGTAGAA9391.0073702166006888No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT9310.9987877227425359No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG9270.9944964758134596No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTACAGATAATACAAG8700.9333462070741205No Hit
CGTATACCACCACTTTTTCCCACAGCATCCCTCCCACCCCGCCGCACAAA8360.8968706081769711No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA8320.8925793612478946No Hit
GGCACTCAGGAAAGTATCTCGTTCACTGACTGGACCCATGTAGAACCATG8130.8721959383347817No Hit
GGTCTCTGAGTCCTGCTGCTGGGAAGTAGCTTCCGTCAGGGCACTGATGG7820.8389387746344394No Hit
AAGAGGCTTCCAAATTGAGGGTTACGGTAGCAAATACAACAGCAAATCAA5960.639395792432386No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC5390.5782455236930472No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG4800.51494963148917No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG4790.5138768197569008No Hit
TATTTATACGAACTGGAAAGATGCTGCTTCTCGGAGTTCTCATCCTGTAG4680.5020758907019407No Hit
CTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG4230.453799362749831No Hit
GCAATGGTTAAGGTGAACTGGTTACACTGACGGGGTGAAGTGGAGATGTA3690.39586752920729945No Hit
TAACATAAGAGATTCTGGGGCTTCAAGAGGTGTACAGGCATCAGGAGAGC3680.3947947174750303No Hit
TTGACATACCTCAGCACACACACACACACACACACACACACACACACACA3480.3733384828296482No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG3210.3443725660583824No Hit
CAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT3200.3432997543261133No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT3160.3390085073970369No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT2970.3186250844839239No Hit
CGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC2910.3121882140903093No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA2910.3121882140903093No Hit
ACTTTTTCCCTAGCTCTGAAGGTTACGGTAGCAGACCTTTGTGCTTTTAT2880.30896977889350197No Hit
AGTGAACTTGATGCTCAGTATTTGCTACCGTAAGGAAGAAAATCAAGGAA2770.29716884983854186No Hit
AAGGGTGCAGTTATGCCTCACCCTAATCCATCCTTTTCCCTTTGTAGAAC2470.26498449787046874No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA2300.24674669842189395No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG2280.24460107495735575No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT2260.24245545149281755No Hit
TCTGGATCAGAGTCAGTGGTGATTGGTCTTCTGGATTTAGCTTTGGGACA2190.2349457693669338No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT2170.23280014590239556No Hit
GCAGTCTGGGGCCCTTTGCTCTGCCACACACAGTCGGGAAACAAGCATAG2160.23172733417012648No Hit
AACCTACCTGTGACTGTGACTCTGCTACCGTAGCCTTAGTGAATGTAAAC2040.21885359338289723No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT1910.20490704086339886No Hit
TATGAGAACACGCAGAGGGAATATTTTTGGTTAATTCAACATCAGTGTAG1810.19417892354070784No Hit
CGTTATAGATATAGACTGCGGAGACCAAGTCTCAGGGCGACTACAGTTCC1590.17057706543078754No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT1590.17057706543078754No Hit
AGGGCTAAAAAGGCTTCTGCTACCGTATCCAGTCCTCAGATAAATGGTGT1540.16521300676944203No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG1440.15448488944675098No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT1310.14053833692725265No Hit
CCACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT1290.13839271346271442No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC1250.13410146653363803No Hit
CAGCTGCCTACTTCCTCCATGGATACAGAAATGGAGGACTTTGTAGAACC1200.1287374078722925No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC1130.12122772574640878No Hit
CGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT1090.11693647881733235No Hit
CTGCCCCCTTACCCTCCACACCCCCAGGATTCTTACAGAAAACAAGTGGT1010.1083539849591795No Hit
TATGGTCCTCAGAAAAGTGCACCCATCACCAGATCATCTTCCATTTGTAA990.10620836149464132No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)