FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110377
Sequences flagged as poor quality0
Sequence length150
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC1542713.976643684825643No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG1389112.585049421528035No Hit
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC79217.176313905976789No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC62375.6506337371010265No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA58965.341692562762169No Hit
AGGACCCCATATAGCACAGGTACATGCAGGCACCTTACCATGGAAGCCAT52384.745553874448481No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT34263.103907516964585No Hit
TCTACCCACTCTCTTTTCAGTGCTCATCAAGCTCATTCTGGGTGCGGAAG33363.0223687905994905No Hit
CTGGTGCTGGAACTCTGGGGTTCTCCCAGGCTCTTACCTGTGGGCATGTT28342.5675638946519657No Hit
TAGAGTAATGACTTTTGTCGGCCATTTCTTAGAATCTTTAGAGTGAAATA27102.455221649437836No Hit
GCAGAGCCAAACCGACCTGAGCATCCCTCAAATGGCACAGCTGCTTAACA25122.2758364514346288No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC19501.7666724045770406No Hit
TGGTGATTTGCTGATATACCATGTCTCCGCAGTCTATATCTATAACTAGA15501.4042780651766218No Hit
TAGTTCATCCCGAGCAAACCTCCCACACTTGGTTTTTCCAGACCAAGTCT11921.0799351314132475No Hit
CTGAGGGAAGCAGCAATTCTCTGCTACCGTACCAAGAGGGGGAAGAGGGA10780.976652744684128No Hit
TCCTCCCCTCTTAGCTGTCTTAAAGAATGGCAGACTGACAGTTGGTCAGA10550.955815070168604No Hit
TTCCTGAGTTTTCATGGACAGCAAGTGAGACCAAGAGAACGTGGGAGTAG9220.8353189523179647No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA9030.8181052211964449No Hit
CCAAATAACAAGTGTTGGAAGCAGGGAAGCTCTTCATCCTCACTAGATAA8190.742002409922357No Hit
CTCATTCTTTCCTTGATTTTCTTCCTTACGGTAGCAAATACTGAGCATCA7760.703045018436812No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTACAT7580.6867372731637932No Hit
CCTCCCCCACCCCCTCTACACACTGACGACATGGTTCTACTCACTCAAAG7570.6858312873152922No Hit
CCTGGATTTGAAAACGGAGCAAAGCGGTTCAGGCGGAGGTGGCTTTAGGT7080.6414379807387408No Hit
AGGTGAACACATGTCTGTGGTAGACACTGACGACATGGTTCTACAAAGTC6980.6323781222537305No Hit
GAGATTGATGACCAAAAGAACTGCTACCGTACTCATGGTGGGGGCAGCAG6310.5716770704041603No Hit
GTATAATGATGAGCATTTTGGCTACCGTACACATAGGTTAACATTTACCA5780.5236598204336048No Hit
CTGGTGTTGTTGGGCAGTAGAGGAGCTTGTCCCGCTAGACCAAGTCTCTG5400.4892323581905651No Hit
GGAAACACTGATGGGCCTGAAGTCCCACGACCCCCTCAGACCAAGTCTCT5370.48651440064506196No Hit
TATTGCTTGAGCTGGCTTCTTTCTGACCCTCAGCAACACTCCATTTGGAA5090.46114679688703264No Hit
TCAAATCATCCATTGCTTGGTACGGTAGCAGAAAGTCAGTCCCATGGAGA5090.46114679688703264No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTACA4100.371454197885429No Hit
ATATTAACTAAATAGGAAAATACCAGCTTCAACCCTCCTAAACCACTGAA3990.3614883535519175No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC3950.3578644101579133No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTACATTCTC3780.34246265073339555No Hit
AAGTGGTTCTGGATTAGCTGGATTATCAGTGCGCTTTTCCCAACACCACC3650.33068483470288196No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTAAGGTAATTAAATGT3610.3270608913088778No Hit
GCAATAAAACTAGTAGTGCAGATACCCAAAAAGTGGCCATTATTGAACTT3560.32253096206637255No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC3360.3044112450963516No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA3350.30350525924785055No Hit
TCTAAGCATAGCATTCAATTTTGGCCACTACAAATCATTCTCCTTAAGAC2960.2681718111563097No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTACATTCT2920.26454786776230554No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCGAGACCAAGT2550.23102639136776681No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC2540.2301204055192658No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTACAT2540.2301204055192658No Hit
TCAAATGTAGCAAATCAGAAGCCAAAGAGGTCACATCCTACAGAGCCCCC2370.214718646094748No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTACATT2270.20565878760973755No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAC2120.19206899988222184No Hit
GAATTGTGCAGAGTGTGGTGTACCATGAAGAATCCCCACCACAATACCAA2080.18844505648821766No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAAATGAAAT2050.1857270989427145No Hit
AGGACATGGCCGCGCATACGGTAGGAGACAGAAGCAGGGAAGACCAAGTC1910.17304329706369986No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG1880.17032533951819673No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCAAC1850.1676073819726936No Hit
AGCAATCTGCTTGTGTGTGCACACTGACGACATGGTTCTGATGAGCCAAG1790.1621714668816873No Hit
ATACGGCCAGGCATTGAAGTACACTGACGACAGGGGCCAGGAGGAGACCA1770.1603594951846852No Hit
CCTATCTCATCGTCTTATATTGGCTTCACTGTCTTTCAACAGGCCTTGCT1630.14767569330567057No Hit
TTAACTACAATTCCCAGCAAGCCAGCATCACACCACCTCAGCAACCAGAC1620.14676970745716952No Hit
GGCTTCCACTTAATTCCAGTCTGGCCAACATGGCAAAACTCGGCCTCTAC1590.1440517499116664No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTACATT1540.13952182066916113No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT1390.12593203294164546No Hit
AGAAGACCAGGAAAGAACGGGAGCTTCTTATGGCCAAGAGACCAAGTCTC1280.11596618860813393No Hit
ACAGAGTTTGAATGGATATTGTGGGTTCGGTAGCAGGGACAGTGATGAAA1260.11415421691113184No Hit
GGGGTCAGAGGCAAGCAGGGGAGCCTCTTGTCTTGTGGGGGTTGTTGACT1240.11234224521412975No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTACATTC1220.11053027351712766No Hit
GGTCTTCTCTGTTTCAGGGCATACGGTAGCAGCATCTTTCCAGTTCGTAT1210.10962428766862663No Hit
TCTATGCTTGTTTCCCGACTGTGTGTGGCAGAGCAAAGGGCCCCAGACTG1180.10690633012312348No Hit
CCTTCCGTTCCAGTTACCGGGACAGGTCATTCTCTCCTGGCTCCTATGAG1130.10237640088061825No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT1130.10237640088061825No Hit
GGTCTCTCATGGCACTGTACTCTTCTTGTCCAGCTGTATCCAGTATGTCC1120.10147041503211719No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)