FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110377
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG1477313.38412893990596No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT1387312.568741676255016No Hit
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG78747.13373257109724No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA62155.630702048434003No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT58145.267401723185084No Hit
ACACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT47884.337860242623011No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC34153.0939416726310736No Hit
TTCTCTTCCGCACCCAGAATGAGCTTGATGAGCACTGAAAAGAGAGTGGG31652.867445210505812No Hit
CCCTGTCCCTCTCTCTTCCTCTCTTCTTCCAGATCTTCAGGGGGCTAGAA27212.465187493771347No Hit
CTATATTTCACTCTAAAGATTCTAAGAAATGGCCGACAAAAGTCATTACT26662.4153582721037896No Hit
GGTCTCTGAGTCCTGCTGCTGGGAAGTAGCTTCCGTCAGGGCACTGATGG24462.2160413854335594No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG19461.7630484611830362No Hit
AGTTATAGATATAGACTGCGGAGACATGGTATATCAGCAAATCACCATGT15341.389782291600605No Hit
GGAAAAACCAAGTGTGGGAGGTTTGCTCGGGATGAACTATGTAGAACCAT11771.0663453436857315No Hit
TCCCTCTTCCCCCTCTTGGTACGGTAGCAGAGAATTGCTGCTTCCCTCAG10540.9549090843201029No Hit
TAACATAAGAGATTCTGGGGCTTCAAGAGGTGTACAGGCATCAGGAGAGC10230.9268235230165706No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT9030.8181052211964449No Hit
TCCGTCTACTCCCACGTTCTCTTGGTCTCACTTGCTGTCCATGAAAACTC8420.7628400844378811No Hit
GCAGATGTCTTTGAGTGAGTAGAACCATGTCGTCAGTGTGTAGAGGGGGT7870.7130108627703236No Hit
AGTCCTAGCCCTTTCACCCATACACATTTGGCTCAGGGTTACCGAAGAGG7820.7084809335278183No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA7540.683113329769789No Hit
AGTGAACTTGATGCTCAGTATTTGCTACCGTAAGGAAGAAAATCAAGGAA7110.644155938284244No Hit
GCTGACCTAAAGCCACCTCCGCCTGAACCGCTTTGCTCCGTTTTCAAATC7090.6423439665872419No Hit
CGCATGGATACAGAAATGGAGGACTTTGTAGAACCATGTCGTCAGTGTCT7030.6369080514962356No Hit
CTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG6330.5734890421011624No Hit
GCTGCCCCCACCATGAGTACGGTAGCAGTTCTTTTGGTCATCAATCTCTG6300.5707710845556592No Hit
AGCGGGACAAGCTCCTCTACTGCCCAACAACACCAGTGTAGAACCATGTC5430.49195031573606823No Hit
AGGGGTGGTAAATGTTAACCTATGTGTACGGTAGCCAAAATGCTCATCAT5410.4901383440390661No Hit
GAGGGGGTCGTGGGACTTCAGGCCCATCAGTGTTTCCTGTAGAACCATGT5250.47564257046304936No Hit
CCATGGGACTGACTTTCTGCTACCGTACCAAGCAATGGATGATTTGATGT5070.4593348251900305No Hit
TCCAAATGGAGTGTTGCTGAGGGTCAGAAAGAAGCCAGCTCAAGCAATAT5040.45661686764452736No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG4120.37326616958243114No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG3950.3578644101579133No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC3780.34246265073339555No Hit
CAACTGTCAGTCTGCCATTCTTTAAGACAGCTAAGAGGGGAGGATCTAAC3600.32615490546037673No Hit
TTTTTCAGTGGTTTAGGAGGGTTGAAGCTGGTATTTTCCTATTTAGTTAA3540.3207189903693704No Hit
CCACTTTGAATGCTCTTTCCTTCCTGGGGATCCAGGGTGTCCACCCAATT3440.31165913188435995No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT3380.3062232167933537No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG3360.3044112450963516No Hit
TGCACGTACCACATTTAATTACCTTACTTGGTCTTCTCTTGGTATTAGTG3350.30350525924785055No Hit
TAAGGAGAATGATTTGTAGTGGCCAAAATTGAATGCTATGCTTAGATGTA2940.26635983945930763No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT2940.26635983945930763No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG2580.23374434891326998No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA2560.23193237721626786No Hit
CGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC2540.2301204055192658No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT2270.20565878760973755No Hit
AGAGACATCTTAAAGAGGGAAGCTGGGGGCTCTGTAGGATGTGACCTCTT2160.19569294327622602No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA2110.1911630140337208No Hit
GGAAAGCTTCTCAAAGTATTTCATTTTGGTCTGTCTAAGAACACAGAGGA2010.18210315554871032No Hit
GTAGTCAGTGCATTCTACAACAGCGCGCATGTTAGCAAGTTCATCAACCA2000.1811971697002093No Hit
TCCCTGCTTCTGTCTCCTACCGTATGCGCGGCCATGTCCTTGTAGAACCA1910.17304329706369986No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT1810.16398343857868938No Hit
CCTCCTGGCCCCTGTCGTCAGTGTACTTCAATGCCTGGCCGTATTGTAGA1760.15945350933618416No Hit
CACAGAGACTTGGCTCATCAGAACCATGTCGTCAGTGTGCACACACAAGC1740.15764153763918207No Hit
CAGCTGTGCCATTTGAGGGATGCTCAGGTCGGTTTGGCTCTGCAGCAGGT1630.14767569330567057No Hit
GGTTGCTGAGGTGGTGTGATGCTGGCTTGCTGGGAATTGTAGTTAATGTA1590.1440517499116664No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT1560.14133379236616325No Hit
TTCTCTTCCGCACCCAGCTAATTTTTGTATTTTTAGTAGAGGCCGAGTTT1410.12774400463864755No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG1380.1250260470931444No Hit
CTTGGCCATAAGAAGCTCCCGTTCTTTCCTGGTCTTCTTGTAGAACCATG1250.11324823106263081No Hit
CTCTCTTCCGCACCCAGAATGAGCTTGATGAGCACTGAAAAGAGAGTGGG1210.10962428766862663No Hit
TCTCTTTAATTTCATCACTGTCCCTGCTACCGAACCCACAATATCCATTC1200.10871830182012557No Hit
GAGTGTTCTGAAAGGAAATCTGGACTACAGAATGTAGAACCATGTCGTCA1200.10871830182012557No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC1180.10690633012312348No Hit
TATTTATACGAACTGGAAAGATGCTGCTACCGTATGCCCTGAAACAGAGA1170.10600034427462243No Hit
GCAGTCTGGGGCCCTTTGCTCTGCCACACACAGTCGGGAAACAAGCATAG1160.10509435842612137No Hit
ACAAGTCAACAACCCCCACAAGACAAGAGGCTCCCCTGCTTGCCTCTGAC1160.10509435842612137No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT1140.10328238672911928No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)