FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40726
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC759218.64165398025831No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC493112.107744438442273No Hit
ATGAGCAAGTTGGGGTGTGCAGGCAGCTGTGGGGGTTGTTGACTTGTAGA19714.839660167951678No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC19474.780729754947699No Hit
TGGTGATAGATGACTTGCTGCTACCGTACAAAGGTTGAATGGTGCTGAAG19334.746353680695379No Hit
CACCATTTATCTGAGGACTGGATACGGTAGGCAGAAGCCTTTTTAGCCCT15583.825565977508226No Hit
TGACCAATGGCTAAGTGAAGATGAACTGACGACATGGTTCTACATGAAAA13273.2583607523449394No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC10622.6076707754260178No Hit
GGAGTTGAACCCAGCCGTGGGATGAAGGCCTGATGTAGGTCTCAGACCAA9872.423513234788587No Hit
TTTCGCTTCCCACAGGTCTCTGCTACCGTAACCCTCAATTTGGAAGCCTC9422.3130187104061286No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT8152.0011786082600795No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA7371.8096547659971516No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTAGAA6731.6525069979865443No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT6281.5420124736040859No Hit
ATAACCCTGTTTTATTGTGTAGATACTTCACCTTCTCTCCTCCCTACTTA4081.0018170210676227No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTAGAAGGAG3780.9281540048126504No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC3570.8765898934341699No Hit
AGAGGAACGTTACACACCAGCAAATGATCAGCCTTGCAAAGACCAAGTCT3530.8667681579335068No Hit
GCCGGAACAGCCCCAGTGTGTGTTGTTCCCCTTCCCTGTCCTTGCCAGAC3180.7808279723027058No Hit
GGGGTCAGAGGCAAGCAGGTACACTGACGACATGGTGGTTGATCTTAAGG2750.675244315670579No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC2560.6285910720424299No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTAGA2500.6138584687914355No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA2490.6114030349162697No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG2390.5868486961646123No Hit
TCTAAGCATAGCATTCAATTTTGGCCACTACAAATCATTCTCCTTAAGAC2120.5205519815351373No Hit
GAGTTTGATGCACAACTTCAAGGCACACTGACTTATGGTATCAAATAATA2020.49599764278347985No Hit
AAATGTCCCAAAGCTAAATCCAGAAGACCAAGTCTCTGCTACCGTAGAAG1930.47389873790698817No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC1920.4714433040318224No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC1900.46653243628149094No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTAGAA1810.44443353140499925No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTAGAAG1800.4419780975298335No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTAGAAGGA1760.43215636202917057No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAG1710.4198791926533418No Hit
ATGAGCAAGTTGGGGTGTGCAGGCAGCTGTGGGGGTTGTTGACTTGGAGA1690.41496832490301033No Hit
ATGAGCAAGTTGGGGTGTGGGGGTTGTTGACTTGTAGACCAAGTCTCTGC1540.37813681677552424No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG1530.37568138290035846No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTAGAAGG1470.36094877964936406No Hit
TCTGGGGTATCAGGTAGGTGTCCCAGCAACAGAGGAGGGGGAGACCAAGT1370.33639444089770665No Hit
TGGGTCCAGTCAGTGAACGAGATACTTTCCTGAGTGCCAGACCAAGTCTC1180.28974119726955755No Hit
TGGTGATAGATGACTTGCTGCTACCGTACAAAGGTTGAATGGTGCTGGAG1180.28974119726955755No Hit
CCTGGATTTGAAAACGGAGCAAAATGCTGCCTTCTTCAATATAAAGACCA1160.28483032951922604No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTAGAAG1150.2823748956440603No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT1110.27255316014339737No Hit
TGATAAATGCACCTGTTTGCTGTTACGGTAGCAGCATCTTGAATCTCATA1100.2700977262682316No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTAGAAGGA1000.24554338751657417No Hit
TTGTCCTGTATATTGGTTCTGTCCCACCCTTATTACCTGGAGAAGACCAA980.24063251976624267No Hit
CACCATTTATCTGAGGACTGGATACGGTAGGCAGAAGCCTTTTTAGCCCG980.24063251976624267No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT910.2234444826400825No Hit
TTAACTACAATTCCCAGCAAGCCACACAGCGGTCATTCTCTCCTGGCTCC880.21607818101458529No Hit
CTGTATGTAGTCGGTGCTGTGAGTAACAGGTAGATGTCATTTCTGAGACC830.20380101163875655No Hit
GTCTCGGAGGCTGGCATCAGACCTGACCTCACCGAGCCCCTACAGTAAGA800.19643471001325935No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTAGAAGG750.18415754063743064No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG690.1694249373864362No Hit
TATTTCTGGACAGTCTACGAAAAGCTCACACTGACGACATGGTGGTTGAT650.15960320188577323No Hit
GACTACAGATGTGCCACCATACCAACTGCTCTGGAACATGTTAGACCAAG620.152236900260276No Hit
TGCTTGGATAGTTGAAATGACGACATCAATCATTCTCTACAGCCCAGACC580.14241516475961302No Hit
TCTATTGTGATGGCCACGTTAGACCAAGTCTCTGCTACCGTAGAAGGAGA530.13013799538378432No Hit
ATGGTTGATAGAGTGGTAGACCTTGTCGTCAGTGTCCTCAGCAACACTCC520.12768256150861856No Hit
CCATTTTCTTCACACCTGTCGTCAGTGTACCAGGTTAGTGTGTAAATCCA520.12768256150861856No Hit
ACACCACTCTCTAAATCAAACACATTGGAGACCAAGTCTCTGCTACCGTA460.11294995825762413No Hit
CCTCAGAAAAAGTAGAAAATGGAAGTCTCTGCTACCGTAAGGAAGCAGAT440.10803909050729264No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT440.10803909050729264No Hit
TTCCTCTATCAGTTTCAGCTAGGTCTCTGCTACCGTAATCAGAACAGAGC430.1055836566321269No Hit
AACTGAGCGACCTCAGAGCCGTTTGTTGTGCTGACCAACATGGTTTGGCT420.10312822275696115No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)