FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40726
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG754718.531159455875855No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA491812.075823798065118No Hit
ACAAGTCAACAACCCCCACAGCTGCCTGCACACCCCAACTTGCTCATTGT19664.827382998575849No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA19394.761086283946373No Hit
TCAGCACCATTCAACCTTTGTACGGTAGCAGCAAGTCATCTATCACCATG19154.702155870942396No Hit
AGGGCTAAAAAGGCTTCTGCCTACCGTATCCAGTCCTCAGATAAATGGTG15443.7911899032559053No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTTCATCTT12673.111034719834995No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG10612.6052153415508523No Hit
GAGACCTACATCAGGCCTTCATCCCACGGCTGGGTTCAACTCCTGTAGAA9832.4136914992879244No Hit
AAGAGGCTTCCAAATTGAGGGTTACGGTAGCAGAGACCTGTGGGAAGCGA8882.180425281147179No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC8011.9668025340077593No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT7331.7998330304964887No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA6731.6525069979865443No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT6281.5420124736040859No Hit
TAAGTAGGGAGGAGAGAAGGTGAAGTATCTACACAATAAAACAGGGTTAT4010.9846289839414624No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC3760.923243137062319No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG3600.883956195059667No Hit
TTGCAAGGCTGATCATTTGCTGGTGTGTAACGTTCCTCTTGTAGAACCAT3500.8594018563080097No Hit
GGCAAGGACAGGGAAGGGGAACAACACACACTGGGGCTGTTCCGGCTGTA3200.7857388400530374No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG2590.635957373667927No Hit
ATGTTACCTTAAGATCAACCACCATGTCGTCAGTGTACCTGCTTGCCTCT2550.6261356381672641No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT2520.6187693365417669No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG2500.6138584687914355No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT2400.589304130039778No Hit
TAAGGAGAATGATTTGTAGTGGCCAAAATTGAATGCTATGCTTAGATGTA2080.5107302460344743No Hit
TCTGGATTTAGCTTTGGGACATTTTGTAGAACCATGTCGTCAGTGTAGAT1980.4861759072828169No Hit
CCTCTAATTTTATTATTTGATACCATAAGTCAGTGTGCCTTGAAGTTGTG1970.4837204734076512No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG1930.47389873790698817No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG1900.46653243628149094No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT1820.446888965280165No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA1800.4419780975298335No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT1800.4419780975298335No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA1720.4223346265285076No Hit
CCAAGTCAACAACCCCCACAGCTGCCTGCACACCCCAACTTGCTCATTGT1690.41496832490301033No Hit
ACAAGTCAACAACCCCCACACCCCAACTTGCTCATTGTAGAACCATGTCG1500.3683150812748613No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC1480.3634042135245298No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT1460.3584933457741983No Hit
CCCCCTCCTCTGTTGCTGGGACACCTACCTGATACCCCAGATGTAGAACC1360.33393900702254087No Hit
CCAGCACCATTCAACCTTTGTACGGTAGCAGCAAGTCATCTATCACCATG1210.2971074988950548No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT1180.28974119726955755No Hit
TTATATTGAAGAAGGCAGCATTTTGCTCCGTTTTCAAATCCAGGTGTAGA1160.28483032951922604No Hit
GGCACTCAGGAAAGTATCTCGTTCACTGACTGGACCCATGTAGAACCATG1160.28483032951922604No Hit
GTATGAGATTCAAGATGCTGCTACCGTAACAGCAAACAGGTGCATTTATC1100.2700977262682316No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG1090.26764229239306586No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT1020.2504542552669057No Hit
CGGGCTAAAAAGGCTTCTGCCTACCGTATCCAGTCCTCAGATAAATGGTG970.23817708589107695No Hit
TCTCCAGGTAATAAGGGTGGGACAGAACCAATATACAGGACAATGTAGAA960.2357216520159112No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT910.2234444826400825No Hit
TAGGAGCCAGGAGAGAATGACCGCTGTGTGGCTTGCTGGGAATTGTAGTT870.21362274713941953No Hit
CAGAAATGACATCTACCTGTTACTCACAGCACCGACTACATACAGTGTAG820.20134557776359083No Hit
TACTGTAGGGGCTCGGTGAGGTCAGGTCTGATGCCAGCCTCCGAGACTGT790.1939792761380936No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC750.18415754063743064No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT660.16205863576093896No Hit
ATGTTACCTTAAGATCAACCACCATGTCGTCAGTGTGAGCTTTTCGTAGA640.15714776801060748No Hit
AACATGTTCCAGAGCAGTTGGTATGGTGGCACATCTGTAGTCTGTAGAAC630.15469233413544173No Hit
GGGCTGTAGAGAATGATTGATGTCGTCATTTCAACTATCCAAGCATGTAG580.14241516475961302No Hit
TGGATTTACACACTAACCTGGTACACTGACGACAGGTGTGAAGAAAATGG520.12768256150861856No Hit
AACGTGGCCATCACAATAGATGTAGAACCATGTCGTCAGTGTAGATCTCG490.12031625988312133No Hit
CCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT470.11540539213278987No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT450.11049452438245838No Hit
TCCAAATGGAGTGTTGCTGAGGACACTGACGACAAGGTCTACCACTCTAT440.10803909050729264No Hit
ACACCGGATATCTGCTTCCTTACGGTAGCAGAGACTTCCATTTTCTACTT420.10312822275696115No Hit
GCTGCCCCCACCATGAGAGCCAAACCATGTTGGTCAGCACAACAAACGGC410.10067278888179541No Hit
TGAACATCCGCTCTGTTCTGATTACGGTAGCAGAGACCTAGCTGAAACTG410.10067278888179541No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)