FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45962
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCACGTTAATTCCCTATCTTGCTGAGCACTTGCATCAGACCAAGTCTC788717.15982768373874No Hit
GTTTATTTGAGGGGAAGTGAAAGAACTTGGACCAAATAGTGAACTGAGAC553012.031678342978983No Hit
ACATGTACATAGGGTTTATATATCATCAGCTATAAGTATCTTCTCTTGTC23025.008485270440799No Hit
CTGAAAGCTGTACCATACCTGTCTGGTCTTGGCTGAGGTTTCAATGAATG20584.477611940298507No Hit
AAGTGGTTCTGGATTAGCTGGATTGTCAGTGCGCTTTTCCCAACACCACC20164.386232104782212No Hit
CCTCAGGGCCTCAGAGAAAGGGTGGGGAAACTTGAAAGTAGACCAAGTCT18614.048996997519691No Hit
AGAGGAACGTTACACACCAGCAAATGATCAGCCTTGCAAAGACCAAGTCT17063.711761890257169No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCAAGACCAAGT11572.517296897437013No Hit
GCTTAGATTCCTCTTTGGAGTCAGTGTACCTCTCACAATATTAAATGCAC10792.3475914886210347No Hit
ACACCACTCTCTAAATCAAACACATTGAAGACCAAGTCTCTGCTACCGTA9522.071276271702711No Hit
GACCTTTTCCTCTGCCCTTATCACAGGTCCAACCCTCCTAAACCACTGAA8911.938557939167138No Hit
TCTCCATATCTCTCTCAATTTCAACAGACCAAGTCTCTGCTACCGTATTG8051.7514468473956748No Hit
TTACCCAGTACCATCAATGCTGTTAATCGGTACAGAGACTTGGTGGACAC7621.657891301509943No Hit
CAGCAGTTACCAGGGCACAGCTTCTCAGTGGTGTTCAAATCAGACCAAGT5471.1901135720812845No Hit
ACGATGGTTGTATTTGTCACCAGGTTGGAGTGCAGAAGCGCTGATCAGAA5031.0943823158261172No Hit
TTCTGATGAGCCAAGTCTCTGTGGGAGGAGGGCAGTACACAGACCAAGTC4290.9333797484878814No Hit
TGAACTTATATATTTGACACAACCCAGCAACCCATTCAGCAGAATTAGAC4160.9050955136852181No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGTTTGC4110.89421696183804No Hit
GGGAAGCTCTTCATCCTCACTAGACCAAGTCTCTGCTACCGTATTGCAGA3990.8681084374048126No Hit
TCTTAAATGGCTACGACCCAGTCTCTGCTACCTGTTAAAGAATCATCTGG3890.8463513337104566No Hit
TCGAATCAAGCACTAATACCAAGAGAAGACCAAGTCTCTGCTACCGTATT3300.7179844219137549No Hit
CTGAGGGAAGCAGCAATTCTCTGCTACCGTACCAAGAGGGGGAAGAGGGA3190.694051607849963No Hit
GGGATAAAAGCACAAAGGTCTGCTACCGTAACCTTCAGAGCTAGGGAAAA3100.6744702145250424No Hit
GGTGCCAGTCTTGCTCACAGAAGGAGGACTCAGACCAAGTCTCTGCTACC3090.6722945041556068No Hit
AAACATAGAAAGTCACTTCTTCTTACTGAGACCAAGTCTCTGCTACCGTA2930.6374831382446369No Hit
GCCTACCAAAATCAGAGAAGCCATACACTGACGACAGGTTTCTTAGAGCT2800.6091989034419738No Hit
TTTTCTATTCTTTCCTTTGTAGTGTCCATAAAGACCAAGTCTCTGCTACC2800.6091989034419738No Hit
TGTTCATTCATGATCCCACTGCAGAGGAAGACAGGGGAAAAGGTACAGAC2750.5983203515947957No Hit
TCAATGATAATAAATTCTCCTCTGTGTTCTTAGACAGACCAAGTCTCTGC2640.5743875375310039No Hit
GTGCACATTCAAAAAAGAAATTATCCAGAAAGACCAAGTCTCTGCTACCG2440.5308733301422914No Hit
TTCCTGCCATCAAGAGTGTCACAGACCAAGTCTCTGCTACCGTATTGCAG2430.5286976197728558No Hit
ATTTACCCTACCAATTGCCCAGTGAGACCAAGTCTCTGCTACCGTATTGC2330.5069405160784997No Hit
GTCCCAAATGGCAATTGTTCCAGTACGGTAGCAGAAACTACCTGCTGCCA2170.47212915016752965No Hit
TGCTGGAAATGTTTTAGATTCTGGTAGACCAAGTCTCTGCTACCGTATTG2150.4677777294286585No Hit
AGGTGAACACATGTCTGTGGTAGGAGACAGAAGCAGGGAAGACCAAGTCT1970.42861494277881723No Hit
CCATTCTTTTCTCTCACACAGGGGATCAGACCAAGTCTCTGCTACCGTAT1960.42643923240938164No Hit
TGGGTTTTGATGTGTAACTTGTCAAGACCAAGTCTCTGCTACCGTATTGC1590.3459379487402637No Hit
GGAAACACTGATGGGCCTGAAAGTCCCACGACCCCCTCAGACCAAGTCTC1590.3459379487402637No Hit
GAGTCACAGTCACAGGTAGGTTAGACCAAGTCTCTGCTACCGTATTGCAG1450.315478003568165No Hit
GCCGGAACAGCCCCAGATGCGGGCCTTGACTCTGGGTTTCCGTCGCATTC1410.30677516209042255No Hit
AGTGGAACTGTTATTATCCCATCAAATGAAGACCAAGTCTCTGCTACCGT1380.30024803098211567No Hit
AGCACCAAACGTAAAGCAGCTACGGTAGCAGAAAGTCAGTCCCATGGAGA1210.2632609547017101No Hit
TCAAGCAATTTAGCAGTTTCAGGAGACCAAGTCTCTGCTACCGTATTGCA1140.24803098211566074No Hit
CCTCAGGGCCTCAGAGAAAGGGTGGGGAAACTTGAAAGGAGACCAAGTCT1100.23932814063791827No Hit
TGAAAAAGAGCAAGGTACTAGTGAGACCAAGTCTCTGCTACCGTATTGCA1090.23715243026848268No Hit
CCCCTATTGCATATTTCTTCATGTGACCAAAGACCAAGTCTCTGCTACCG1090.23715243026848268No Hit
AGAGGAACGTTACACACCAGCAAATGATCAGCCTTGCAGAGACCAAGTCT1040.22627387842130453No Hit
ACTCCAAAAAATACTAAGAGGCCCAAGGAGGTGAGTCATTCAGTAATCAC1000.21757103694356206No Hit
GAAGTATTACAAATGGAAGATGATCTGGTGTGGAGGCTGGGAGGTCCAAA910.19798964361864146No Hit
CAGCAGTTACCAGGGCACAGCTTCAGCATACTTAAATATTCCTGCCATCA880.1914625125103346No Hit
GGAGTATAAAGTAATATGGATGAAGAAAGGCCCGAGACCAAGTCTCTGCT870.18928680214089902No Hit
AGCTGCTCACCATCGCTACCGTAAGGTCTTGGCCAGTTGGAGACCAAGTC810.17623253992428528No Hit
GGGAAAAGAACAGGCTTCACCTACACTGACGACATGGTTCTAAACTGCCA800.17405682955484966No Hit
ATATATGTCTTCCACCCTTGACTCTCACACTGACGACATGGTTCTACATC800.17405682955484966No Hit
TCTATTGTGATGGCCACGTTAGACCAAGTCTCTGCTACCGTATTGCAGAT790.17188111918541404No Hit
CTGGTTCAGAGGAAGTATCATGTCGTCAGTGTAGCACAGCAAGTGGAAAA770.1675296984465428No Hit
TCCCTGCCAGCGAGATCATGGTTCTACATTCCTGCCATCAAGAGTGTCAC740.16100256733823592No Hit
CCCTCAGATGTTATTTTCCAAGCACAGCTTTTATATTTCCGAGACCAAGT670.1457725947521866No Hit
CTGGTTCAGAGGAAGTATCATGTCGTCAGTGTGAGGCATAACTGCACCCT640.13924546364387974No Hit
GATTCCCGCGCCCAGAGACCAAGTCTCTGCTACCGTATTGCAGATCAATC630.13706975327444412No Hit
GGAAACACTGATGGGCCTGAAGCACACAGAGGAGCAGTAGAGTCCCACGA590.12836691179670162No Hit
AATCTCCAAGGAAGTTGTACCGTCTTAGACCAAGTCTCTGCTACCGTATT570.12401549105783037No Hit
GAGAAAGCCCCCCTACTGCTACCGTACCTTCCACTCGGATAAGATGCTAG560.12183978068839477No Hit
ACACCACTCTCTAAATCAAACACATTGGAGACCAAGTCTCTGCTACCGTA560.12183978068839477No Hit
AAGGGAAAAGGATGGATTAGGGTTACGGTAGCACAGCAAGTGGAAAATCT550.11966407031895913No Hit
CAGCAGTTACCAGGGCACAGCTTCTCATGGTGGGGGCAGCAGACCAAGTC550.11966407031895913No Hit
CCAAGCTACATATTGCAGAAGAGTACATTTGAAGTGCAGACCAAGTCTCT540.11748835994952352No Hit
TCCTTAATGATCAGGGCATTTCTAGAGACTTGGTCTCCGCAGTCTATATC530.11531264958008791No Hit
GCCTACCAAAATCAGAGAAGCCATACGCTGACGACAGGTTTCTTAGAGCT480.1044340977329098No Hit
CACATCCTCCTAAACACACAGGCTCTCTCAGAATGTTAGTACTGTTTGAA470.10225838736347417No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)