FastQCFastQC Report
Sun 9 Jul 2023
EGAF00008102252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008102252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45962
Sequences flagged as poor quality0
Sequence length150
%GC39

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGCAAGTGCTCAGCAAGATAGGGAATTAACGTGATCTGTAGAACCATG784017.057569296375267No Hit
CAGTTCACTATTTGGTCCAAGTTCTTTCACTTCCCCTCAAATAAACTGTA553712.046908315565032No Hit
CCATGGACAAGAGAAGATACTTATAGCTGATGATATATAAACCCTATGTA22814.962795352682651No Hit
GTTGATACAAAACAAGCCCACGAACTGGCCAAGAGTTACGGGATTCCATT20364.4297463121709235No Hit
ACTTTCAAGTTTCCCCACCCTTTCTCTGAGGCCCTGAGGTGTAGAACCAT18494.0228884730864625No Hit
TTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG18223.964144293111701No Hit
TTGCAAGGCTGATCATTTGCTGGTGTGTAACGTTCCTCTTGTAGAACCAT16963.690004786562813No Hit
TGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC11402.4803098211566077No Hit
TGAAGTGCATTTAATATTGTGAGAGGTACACTGACTCCAAAGAGGAATCT9602.088681954658196No Hit
TCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT9492.064749140594404No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTAGA8091.7601496888734172No Hit
TTTTTCAGTGGTTTAGGAGGGTTGGACCTGTGATAAGGGCAGAGGAAAAG7701.675296984465428No Hit
GTGTACACAGTGTCCACCAAGTCTCTGTACCGATTAACAGCATTGATGGT7521.6361341978155868No Hit
GATTTGAACACCACTGAGAAGCTGTGCCCTGGTAACTGCTGTGTAGAACC5441.1835864409729775No Hit
TTCTGATCAGCGCTTCTGCACTCCAACCTGGTGACAAATACAACCATCGT4971.0813280536095033No Hit
GTGTACTGCCCTCCTCCCACAGAGACTTGGCTCATCAGAATGTAGAACCA4270.92902832774901No Hit
AATTCTGCTGAATGGGTTGCTGGGTTGTGTCAAATATATAAGTTCATGTA4130.8985683825769114No Hit
AGTGAGGATGAAGAGCTTCCCTGTAGAACCATGTCGTCAGTGTAGATCTC3930.8550541751881989No Hit
GAGTCCTCCTTCTGTGAGCAAACAGAGACTTGGTCTGAGTCCTCCTTCTG3920.8528784648187633No Hit
TCCAGATGATTCTTTAACAGGTAGCAGAGACTGGGTCGTAGCCATTTAAG3460.7527957878247248No Hit
TCTCTTGGTATTAGTGCTTGATTCGATGTAGAACCATGTCGTCAGTGTAG3320.7223358426526261No Hit
TCCCTCTTCCCCCTCTTGGTACGGTAGCAGAGAATTGCTGCTTCCCTCAG3140.6831730560027849No Hit
GAGTCCTCCTTCTGTGAGCAAGACTGGCACCTGTAGAACCATGTCGTCAG3060.6657673730472999No Hit
CAGTAAGAAGAAGTGACTTTCTATGTTTTGTAGAACCATGTCGTCAGTGT2990.6505374004612506No Hit
TTATGGACACTACAAAGGAAAGAATAGAAAATGTAGAACCATGTCGTCAG2760.6004960619642313No Hit
GTACCTTTTCCCCTGTCTTCCTCTGCAGTGGGATCATGAATGAACATGTA2760.6004960619642313No Hit
ACTTTTTCCCTAGCTCTGAAGGTTACGGTAGCAGACCTTTGTGCTTTTAT2740.59614464122536No Hit
CAGGACTAGCTCTAAGAAACCTGTCGTCAGTGTATGGCTTCTCTGATTTT2740.59614464122536No Hit
GTCTAAGAACACAGAGGAGAATTTATTATCATTGATGTAGAACCATGTCG2640.5743875375310039No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGTCGTCAGTGTAGATCT2470.5374004612505984No Hit
CACTGGGCAATTGGTAGGGTAAATTGTAGAACCATGTCGTCAGTGTAGAT2390.5199947782951133No Hit
TTCTGGATAATTTCTTTTTTGAATGTGCACTGTAGAACCATGTCGTCAGT2350.511291936817371No Hit
GGTGGCAGCAGGTAGTTTCTGCTACCGTACTGGAACAATTGCCATTTGGG2170.47212915016752965No Hit
ACCAGAATCTAAAACATTTCCAGCATGTAGAACCATGTCGTCAGTGTAGA2160.46995343979809406No Hit
TCCCTGCTTCTGTCTCCTACCACAGACATGTGTTCACCTTGTAGAACCAT1990.4329663635176885No Hit
GATCCCCTGTGTGAGAGAAAAGAATGGTGTAGAACCATGTCGTCAGTGTA1940.4220878116705104No Hit
TGACAAGTTACACATCAAAACCCATGTAGAACCATGTCGTCAGTGTAGAT1570.34158652800139244No Hit
GAGGGGGTCGTGGGACTTTCAGGCCCATCAGTGTTTCCTGTAGAACCATG1540.3350593968930856No Hit
AACCTACCTGTGACTGTGACTCTGTAGAACCATGTCGTCAGTGTAGATCT1420.30895087245985814No Hit
TCATTTGATGGGATAATAACAGTTCCACTTGTAGAACCATGTCGTCAGTG1370.29807232061268No Hit
CTGGGCGCGGGAATGCGACGGAAACCCAGAGTCAAGGCCCGCATCTGGGG1360.2958966102432444No Hit
CCATGGGACTGACTTTCTGCTACCGTAGCTGCTTTACGTTTGGTGCTTGT1200.2610852443322745No Hit
GGCATGACTTGGCAGTTTAGAACCATGTCGTCAGTGTAGGTGAAGCCTGT1170.2545581132239676No Hit
CCTGAAACTGCTAAATTGCTTGATGTAGAACCATGTCGTCAGTGTAGATC1140.24803098211566074No Hit
CCTTTCAAGTTTCCCCACCCTTTCTCTGAGGCCCTGAGGTGTAGAACCAT1100.23932814063791827No Hit
TTGGTCACATGAAGAAATATGCAATAGGGGTGTAGAACCATGTCGTCAGT1090.23715243026848268No Hit
CACTAGTACCTTGCTCTTTTTCATGTAGAACCATGTCGTCAGTGTAGATC1070.2328010095296114No Hit
CGAAGTGCATTTAATATTGTGAGAGGTACACTGACTCCAAAGAGGAATCT1050.22844958879074018No Hit
CTGCAAGGCTGATCATTTGCTGGTGTGTAACGTTCCTCTTGTAGAACCAT990.21539532657412647No Hit
GTGACACTCTTGATGGCAGGAATATTTAAGTATGCTGAAGCTGTGCCCTG950.20669248509638397No Hit
TTGTGATTACTGAATGACTCACCTCCTTGGGCCTCTTAGTATTTTTTGGA920.2001653539880771No Hit
GTTGAAATTGAGAGAGATATGGAGATGTAGAACCATGTCGTCAGTGTGAG830.18058396066315652No Hit
CGGGCCTTTCTTCATCCATATTACTTTATACTCCTGTAGAACCATGTCGT830.18058396066315652No Hit
ACCTGATGAAGACTTTGGACCTCCCAGCCTCCACACCAGATCATCTTCCA830.18058396066315652No Hit
CCAACTGGCCAAGACCTTACGGTAGCGATGGTGAGCAGCTTGTAGAACCA820.1784082502937209No Hit
AACGTGGCCATCACAATAGATGTAGAACCATGTCGTCAGTGTAGATCTCG800.17405682955484966No Hit
GTGACACTCTTGATGGCAGGAATGTAGAACCATGATCTCGCTGGCAGGGA760.1653539880771072No Hit
CTCCAACAGGTTCTTGCTGGTGTGAAATGACTGAGTACAAACTGGTGGTG710.15447543622992907No Hit
ACAGATTTTCCACTTGCTGTGCTACACTGACGACATGATACTTCCTCTGA700.15229972586049345No Hit
CGGAAATATAAAAGCTGTGCTTGGAAAATAACATCTGAGGGTGTAGAACC660.14359688438275098No Hit
CTGGGCGCGGGAATCTGTAGAACCATGTCGTCAGTGTAGATCTCGGTGGT640.13924546364387974No Hit
AAGGGTGCAGTTATGCCTCACACTGACGACATGATACTTCCTCTGAACCA640.13924546364387974No Hit
TTCCAACAGGTTCTTGCTGGTATGAAATGACTGAGTACAAACTGGTGGTG630.13706975327444412No Hit
GCACTTCAAATGTACTCTTCTGCAATATGTAGCTTGGTGTAGAACCATGT580.126191201427266No Hit
GCTGCCCCCACCATGAGAAGCTGTGCCCTGGTAACTGCTGTGTAGAACCA570.12401549105783037No Hit
AGCATCTTATCCGAGTGGAAGGTACGGTAGCAGTAGGGGGGCTTTCTCTG560.12183978068839477No Hit
GAGGGGGTCGTGGGACTCTACTGCTCCTCTGTGTGCTTCAGGCCCATCAG560.12183978068839477No Hit
AAGACGGTACAACTTCCTTGGAGATTTGTAGAACCATGTCGTCAGTGTAG560.12183978068839477No Hit
CCAATGTGTTTGATTTAGAGAGTGGTGTTGTAGAACCATGTCGTCAGTGT560.12183978068839477No Hit
ACAGATTTTCCACTTGCTGTGCTACCGTAACCCTAATCCATCCTTTTCCC510.11096122884121666No Hit
AGATCCACAGAACTTATAAAAACCTTTGTGTGTAGAACCATGTCGTCAGT490.10660980810234541No Hit
CAGGACTAGCTCTAAGAAACCTGTCGTCAGCGTATGGCTTCTCTGATTTT490.10660980810234541No Hit
GGGTGTGAGATGTTTATCTAACAATGACTCAAATCTTGATTGTTTTAATT480.1044340977329098No Hit
AGTTATAGATATAGACTGCGGAGACCAAGTCTCTAGAAATGCCCTGATCA480.1044340977329098No Hit
GCAAGGTCTACCACTCTATCAACCATTGTAGAACCATGTCGTCAGTGTAG470.10225838736347417No Hit
ACCTCATCAGAATGGTAGGAATAGCTGTAGAACCATGTCGTCAGTGTAGA460.10008267699403856No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)